Virus Genes

, Volume 48, Issue 1, pp 168–173 | Cite as

Nucleotide sequence diversity in Velvet tobacco mottle virus: a virus with a unique Australian pathosystem

  • K. Arthur
  • N. C. Collins
  • A. Yazarlou
  • J. W. Randles
Article

Abstract

Velvet tobacco mottle virus (VTMoV) is a naturally occurring mirid-transmitted sobemovirus of native velvet tobacco (Nicotiana velutina) plants in the Australian arid zone. We have sequenced the coding region of a typical field isolate of VTMoV (isolate I-17-04, satellite-plus) and show that it differed by nine polymorphisms from the previously sequenced atypical ‘satellite-minus’ variant VTMoV-K1 (represented here as L-K1-04), while retaining the same genomic and amino acid sequence motifs. We also report that although L-K1-04 was confirmed to be free of detectable satellite RNA by gel electrophoretic assay, the satellite sequence was detected in it by RT-PCR assay. Nucleotide sequence variation among the RNA-dependent RNA polymerase open reading frames of 15 field and laboratory isolates identified four phylogenetic groups, but these did not show a pattern related to site or time of sampling. This result would be consistent with nucleotide sequence variants of VTMoV being dispersed widely by migrating adult mirid vectors.

Keywords

Native plant virus RdRp Satellite RNA Sobemovirus Velvet tobacco mottle virus Virus sequence variation 

Supplementary material

11262_2013_1007_MOESM1_ESM.docx (218 kb)
Supplementary material 1 (DOCX 217 kb)

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • K. Arthur
    • 1
    • 2
  • N. C. Collins
    • 3
  • A. Yazarlou
    • 1
  • J. W. Randles
    • 1
  1. 1.School of Agriculture, Food and WineUniversity of AdelaideGlen OsmondAustralia
  2. 2.New Zealand Institute of Plant & Food Research LtdAucklandNew Zealand
  3. 3.Australian Centre for Plant Functional Genomics (ACPFG), School of Agriculture Food and WineUniversity of AdelaideGlen OsmondAustralia

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