Virus Genes

, Volume 47, Issue 2, pp 235–243 | Cite as

Construction and characterization of an infectious cDNA clone of enterovirus type 71 subgenotype C4

  • Yong-Xin Zhang
  • Tao Wei
  • Xiao-Yu Li
  • Xiao Yin
  • Yu-Huan Li
  • Ji-Wei Ding
  • Jin-Ming Zhou
  • Guang-Zhou Zhang
  • Qi JinEmail author
  • Shan CenEmail author


Enterovirus 71 (EV71) is the major causative agent of hand, foot, and mouth disease and induces fatal neurological complications. In recent years, this virus has become a major threat to public health in the Asia–Pacific region, while no effective antiviral therapies and vaccines are currently available. In this study, we constructed and characterized for the first time an infectious full-length EV71 cDNA clone derived from the SHZH98 strain, which was the first subgenotype C4 strain isolated in China. Our data demonstrate that the rescued EV71 viruses exhibited growth kinetics in vitro and morphologies similar to those of the BrCr-TR strain and reached a maximum titer of 107.5 TCID50/ml. Although the rescued viruses were able to infect suckling mice, no typical symptoms of EV71 infection were observed for up to 18 days post-inoculation. Taken together our research provides an important tool to study the epidemic strains of EV71 in the Asia–Pacific region and promote the development of vaccines.


Enterovirus 71 Infectious cDNA clone Subgenotype C4 SHZH98 strain 



The authors thank Dr. Lawrence Kleiman at the Lady Davis Institute for proofreading. This study was supported in part by the National 973 Grant—2012CB911100 (to C.S.), the Nature Science Foundation of China—30973569 (to S.C.), the National S&T Major Special Project on Major New Drug Innovation—2012ZX09301-002-004 (to C.S.), and the Xiehe Scholar fund (to S.C.).


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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Yong-Xin Zhang
    • 1
  • Tao Wei
    • 2
  • Xiao-Yu Li
    • 1
  • Xiao Yin
    • 1
  • Yu-Huan Li
    • 1
  • Ji-Wei Ding
    • 1
  • Jin-Ming Zhou
    • 1
  • Guang-Zhou Zhang
    • 3
  • Qi Jin
    • 4
    Email author
  • Shan Cen
    • 1
    Email author
  1. 1.Institute of Medicinal BiotechnologyChinese Academy of Medical Science and Peking Union Medical SchoolBeijingPeople’s Republic of China
  2. 2.Beijing Union UniversityBeijingPeople’s Republic of China
  3. 3.Laboratory Animal Center of the Academy of Military Medical SciencesBeijingPeople’s Republic of China
  4. 4.Institute of Pathogen BiologyChinese Academy of Medical Science and Peking Union Medical SchoolBeijingPeople’s Republic of China

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