Advertisement

Virus Genes

, Volume 47, Issue 2, pp 311–316 | Cite as

Isolation and molecular characterization of Newcastle disease virus genotypes II and VIId in Egypt between 2011 and 2012

  • M. M. Radwan
  • S. F. Darwish
  • I. M. El-SabaghEmail author
  • A. A. El-Sanousi
  • M. A. Shalaby
Article

Abstract

The current study was conducted to isolate and characterize Newcastle disease virus (NDV) from recent outbreaks affecting poultry farms in Egypt between 2011 and 2012. Trachea, spleen, liver, proventriculus and caecal tonsils were collected from clinically infected NDV ten different vaccinated broiler farms in Fayoum, Behira and Giza Provinces. Inoculation of all the collected samples in 10-day-old embryonated chicken specific-pathogen-free eggs resulted in isolation of haemagglutinating agents in three samples. These haemagglutinating agents were confirmed as NDV by real-time reverse transcription polymerase chain reaction (rt RT-PCR) using matrix (M) gene-specific primers. The deduced amino acid sequences of the fusion protein revealed that one isolate possessed the motif 112RRQKRF117 at the cleavage site, indicating that this isolate is velogenic genotype, whereas the other two isolates carries the motif 112GRQGRL117 indicating they are lentogenic genotype. The phylogenetic analysis revealed that the velogenic genotype isolate clustered with published class II genotype VII sub genotype d NDVs and closely related to Middle East isolates, whereas the other two isolates clustered with published class II genotype II NDVs. The spread of velogenic genotype strain to Egypt via Middle Eastern countries is likely to be the source of infection.

Keywords

Newcastle disease virus Velogenic and lentogenic genotypes Egypt 

References

  1. 1.
    OIE. Newcastle disease virus. OIE Terrestrial Manual, chap 2.3.14 (OIE, Paris, 2012)Google Scholar
  2. 2.
    E.W. Aldous, D.J. Alexander, Avian Pathol. 30, 117–128 (2001)PubMedCrossRefGoogle Scholar
  3. 3.
    R.A. Lamb, G.D. Parks, in Fields Virology, 5th edn, ed by D.M. Knipe, P.M Howley (Lippincott Williams & Wilkins, Philadelphia, 2007), pp. 1450–1496Google Scholar
  4. 4.
    A. Czeglédi, D. Ujvari, E. Somogyi, E. Wehmann, O. Werner, B. Lomniczi, Virus Res. 120, 36–48 (2006)PubMedCrossRefGoogle Scholar
  5. 5.
    D.J. Alexander, in Diseases of Poultry, 9th edn, ed by B.W. Calnek, H.J. Barnes, C.W. Beard, W.M. Reid, H.W. Yoder Jr (Iowa State University Press, Ames, 1997) pp. 541–570Google Scholar
  6. 6.
    O. de Leeuw, B. Peeters, J. Gen. Virol. 80, 131–136 (1999)PubMedGoogle Scholar
  7. 7.
    R.S. De Almeida, O.F. Maminiaina, P. Gil, S. Hammoumi, S. Molia, V. Chevalier, M. Koko, H.R. Andriamanivo, A. Traore, K. Samake, A. Diarra, C. Grillet, D. Martinez, E. Albina, Vaccine 27, 3127–3129 (2009)CrossRefGoogle Scholar
  8. 8.
    P.J. Miller, E.L. Decanini, C.L. Afonso, Infect. Genet. Evol. 10, 26–35 (2010)PubMedCrossRefGoogle Scholar
  9. 9.
    D.J. Alexander, Revuescientifique et technique (International Office of Epizootics) 19, 443–462 (2000)Google Scholar
  10. 10.
    M.G. Wise, D.L. Suarez, B.S. Seal, J.C. Pedersen, D.A. Senne, D.J. King, D.R. Kapczynski, E. Spackman, J. Clin. Microbiol. 42, 329–338 (2004)PubMedCrossRefGoogle Scholar
  11. 11.
    B.S. Seal, Virus Genes 11, 217–224 (1995)PubMedCrossRefGoogle Scholar
  12. 12.
    L.M. Kim, D.J. King, D.L. Suarez, C.W. Wang, C.L. Afonso, J. Clin. Microbiol. 45, 1310–1314 (2007)PubMedCrossRefGoogle Scholar
  13. 13.
    Z. Rui, P. Juan, S. Jingliang, Z. Jixun, W. Xiaoting, Z. Shouping, L. Xiaojiao, Z. Guozhong, Vet. Microbiol. 141, 246–257 (2010)PubMedCrossRefGoogle Scholar
  14. 14.
    N. Jindal, Y. Chander, A.K. Chockalingam, M. de Abin, P.T. Redig, S.M. Goyal, Virol. J. 6, 191 (2009)PubMedCrossRefGoogle Scholar
  15. 15.
    M.M.K. Ababneh, A.E. Dalab, S.R. Alsaad, M. B. Al-Zaghloul, Res. Vet. Sci.,93/3 : 1512-1514 (2012)Google Scholar
  16. 16.
    M.H.A. Mohamed, S. Kumar, A. Paldurai, M.M. Megahed, I.A. Ghanem, M.A. Lebdah, S.K. Samal, Virus Genes 39, 234–237 (2009)PubMedCrossRefGoogle Scholar
  17. 17.
    M.H.A. Mohamed, S. Kumar, A. Paldura, S.K. Samal, Virol. J. 8, 237 (2011)PubMedCrossRefGoogle Scholar
  18. 18.
    H.A. Hussein, A.A. El-Sanousi, A.A. Youssif, M.A. Shalaby, M.S. Saber, I.M. Reda, Int. J. Virol. 1, 38 (2005)CrossRefGoogle Scholar
  19. 19.
    A.A.S. Ahmed, M.S. Sabban, M.M. Ibrahim, A. Amin, A.R. Khafagi, A. Sheble, Zentralbl. Veterinarmed. [B] 27, 313–319 (1980)CrossRefGoogle Scholar
  20. 20.
    M.S. Sabban, A.A. Zied, A. Basyouni, S. Nadiem, N. Barhouma, Y.Z. Habashi, Zentralbl. Veterinarmed. [B] 29, 193–198 (1982)CrossRefGoogle Scholar
  21. 21.
    P. Gilchrist, World’s poult. Sci. J. 61, 198–214 (2005)CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • M. M. Radwan
    • 1
  • S. F. Darwish
    • 2
  • I. M. El-Sabagh
    • 3
    • 4
    Email author
  • A. A. El-Sanousi
    • 3
  • M. A. Shalaby
    • 3
  1. 1.Veterinary Laboratory DepartmentArab Poultry Breeders Co. “OMMAT”GizaEgypt
  2. 2.Biotechnology Research Unit, Animal Reproduction Research InstituteAgriculture Research CenterGizaEgypt
  3. 3.Department of Virology, Faculty of Veterinary MedicineCairo UniversityGizaEgypt
  4. 4.Central Biotechnology Laboratory, College of Veterinary Medicine and Animal ResourcesKing Faisal UniversityAl-HufofSaudi Arabia

Personalised recommendations