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Virus Genes

, Volume 46, Issue 3, pp 473–478 | Cite as

Two natural recombinant highly pathogenic porcine reproductive and respiratory syndrome viruses with different pathogenicities

  • Nanhua Chen
  • Xiuling Yu
  • Lilin Wang
  • Jiajun Wu
  • Zhi Zhou
  • Jianqiang Ni
  • Xiangdong Li
  • Xinyan Zhai
  • Kegong TianEmail author
Article

Abstract

Highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) is a variant of type 2 PRRSV with high virulence. Genetic and pathogenic characteristics of HP-PRRSV vary rapidly during the evolution. In this study, we determined the complete genome of a HP-PRRSV isolate designated 10FUJ-2, which shared 98.34 % nucleotide identity with HP-PRRSV reference strain JXA1. Genomic analyses by phylogenetic tree and recombination detection program confirmed 10FUJ-2 to be a recombinant with 09JS and JXA1 as potential parental viruses. Furthermore, we identified that 10FUJ-2 has high virulence as similar as the parental viruses by animal challenge study. In addition, we found that SY0909 was also a recombination virus probably from JXA1 and NT0801, which has been reported to be low pathogenic. Recombination analysis also revealed that Glycoproteins GP2 to GP5 of HP-PRRSV might contain major virulence determinants. Identification of two natural recombinants with different virulence supports the notion that recombination is a driving force affecting HP-PRRSV pathogenicity and a common mechanism contributing to HP-PRRSV evolution.

Keywords

HP-PRRSV Recombination Pathogenicity Evolution 

Notes

Acknowledgments

This work was supported by grants from the National Scientific and Technical Supporting Programs (2006BAD06A07, 2008FY130100-2 and 2009BADB4B05-3) and the Scientific Achievement Transformation Program (2009GB23260435).

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Nanhua Chen
    • 1
    • 2
  • Xiuling Yu
    • 1
  • Lilin Wang
    • 1
  • Jiajun Wu
    • 1
  • Zhi Zhou
    • 1
  • Jianqiang Ni
    • 1
  • Xiangdong Li
    • 3
  • Xinyan Zhai
    • 1
  • Kegong Tian
    • 1
    Email author
  1. 1.Veterinary Diagnostic LabChina Animal Disease Control CenterBeijingPeople’s Republic of China
  2. 2.Department of Diagnostic Medicine and PathobiologyCollege of Veterinary Medicine, Kansas State UniversityManhattanUSA
  3. 3.Department of Anatomy and PhysiologyCollege of Veterinary Medicine, Kansas State UniversityManhattanUSA

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