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Virus Genes

, Volume 46, Issue 1, pp 167–169 | Cite as

The effects of the 11 kDa protein and the putative X protein on the p6 promoter activity of Parvovirus B19 in Hela cells

  • Yanming Dong
  • Yu Huang
  • Ying Wang
  • Peng Xu
  • Yongbo Yang
  • Kaiyu Liu
  • Peter Tijssen
  • Jianxin PengEmail author
  • Yi LiEmail author
Article

Abstract

Human parvovirus B19 (B19) is a small nonenveloped icosahedral virus with a single-stranded, linear 5.6 kb DNA genome. The p6 promoter, at map unit 6 of the viral genome, controls the expression of all B19 transcripts. Some previous reports revealed that this promoter is transactivated by NS1 protein. In an attempt to investigate the roles of other small viral proteins in the control of the p6 promoter activity, various truncated promoter/reporter constructs along with these nonstructural protein expression vectors were introduced into Hela cells. The results showed that the putative X protein upregulated the activity of p6 promoter significantly, but that the 11 kDa protein did not. Furthermore, the possible responsive DNA elements for X protein were identified to be located primarily between nt 265 and 343 of the p6 promoter region. In addition, we observed that deletion of the potential ATF/CREB binding sites located in 5′ terminal nucleotide influenced the activity of p6 promoter significantly.

Keywords

Parvovirus B19 p6 promoter 11 kDa protein X protein 

Notes

Acknowledgments

We thank Prof. Dr. Lulin Li for his helpful suggestion. This work was funded by the National Natural Science Foundation of China (30670081).

Supplementary material

11262_2012_839_MOESM1_ESM.doc (4.9 mb)
Supplementary material 1 (DOC 4993 kb)

References

  1. 1.
    P. Tijssen, M. Agbandje-McKenna, J.M. Almendral, M. Bergoin, T.W. Flegel, K. Hedman, J.A. Kleinschmidt, Y. Li, D.J. Pintel, P. Tattersall, Parvoviridae, in Virus taxonomy: classification and nomenclature of viruses. Ninth report of the International Committee on Taxonomy of Viruses, ed. by A.M.Q. King, M.J. Adams, E. Carstens, E.J. Lefkowitz (Elsevier, San Diego, 2011), pp. 375–395Google Scholar
  2. 2.
    F. Wang, Y. Wei, C. Zhu, X. Huang, Y. Xu, L. Yu, X. Yu, Virus Genes 41, 305–308 (2010)PubMedCrossRefGoogle Scholar
  3. 3.
    A.D. Woolf, G.V. Campion, A. Chishick, S. Wise, B.J. Cohen, P.T. Klouda, O. Caul, P.A. Dieppe, Arch. Intern. Med. 149, 1153–1156 (1989)PubMedCrossRefGoogle Scholar
  4. 4.
    A.U. Sheikh, J.M. Ernest, Ann. Med. 27, 7–8 (1995)PubMedCrossRefGoogle Scholar
  5. 5.
    N.S. Young, K.E. Brown, Parvovirus B19. N. Engl. J. Med. 350, 586–597 (2004)PubMedCrossRefGoogle Scholar
  6. 6.
    M.C. Blundell, C. Beard, C.R. Astell, Virology 157, 534–538 (1987)PubMedCrossRefGoogle Scholar
  7. 7.
    K. Ozawa, J. Ayub, Y.S. Hao, G. Kurtzman, T. Shimada, N. Young, J. Virol. 61, 2395–2406 (1987)PubMedGoogle Scholar
  8. 8.
    R. Gareus, A. Gigler, A. Hemauer, M. Leruez-Ville, F. Morinet, H. Wolf, S. Modrow, J. Virol. 72, 609–616 (1998)PubMedGoogle Scholar
  9. 9.
    M. Momoeda, M. Kawase, S.M. Jane, K. Miyamura, N.S. Young, S. Kajigaya, J. Virol. 68, 7159–7168 (1994)PubMedGoogle Scholar
  10. 10.
    U. Raab, B. Bauer, A. Gigler, K. Beckenlehner, H. Wolf, S. Modrow, J. Gen. Virol. 82, 1473–1480 (2001)PubMedGoogle Scholar
  11. 11.
    M.C. Blundell, C.R. Astell, J. Virol. 63, 4814–4823 (1989)PubMedGoogle Scholar
  12. 12.
    M. Momoeda, S. Wong, M. Kawase, N.S. Young, S. Kajigaya, J. Virol. 68, 8443–8446 (1994)PubMedGoogle Scholar
  13. 13.
    E. Morita, A. Nakashima, H. Asao, H. Sato, K. Sugamura, J. Virol. 77, 2915–2921 (2003)PubMedCrossRefGoogle Scholar
  14. 14.
    S. Moffatt, N. Yaegashi, K. Tada, N. Tanaka, K. Sugamura, J. Virol. 72, 3018–3028 (1998)PubMedGoogle Scholar
  15. 15.
    S. Moffatt, N. Tanaka, K. Tada, M. Nose, M. Nakamura, O. Muraoka, T. Hirano, K. Sugamura, J. Virol. 70, 8485–8491 (1996)PubMedGoogle Scholar
  16. 16.
    U. Raab, K. Beckenlehner, T. Lowin, H.H. Niller, S. Doyle, S. Modrow, Virology 293, 86–93 (2002)PubMedCrossRefGoogle Scholar
  17. 17.
    A.Y. Chen, E.Y. Zhang, W. Guan, F. Cheng, S. Kleiboeker, T.M. Yankee, J. Qiu, Blood 115, 1070–1080 (2009)PubMedCrossRefGoogle Scholar
  18. 18.
    H. Kirby, A. Rickinson, A. Bell, J. Gen. Virol. 81, 1057–1066 (2000)PubMedGoogle Scholar
  19. 19.
    R. Fahraeus, L. Palmqvist, A. Nerdstedt, S. Farzad, L. Rymo, S. Lain, EMBO J. 13, 6041–6051 (1994)PubMedGoogle Scholar
  20. 20.
    N. Zhi, I.P. Mills, J. Lu, S. Wong, C. Filippone, K.E. Brown, J. Virol. 80, 5941–5950 (2006)PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2012

Authors and Affiliations

  • Yanming Dong
    • 1
  • Yu Huang
    • 1
  • Ying Wang
    • 1
  • Peng Xu
    • 1
  • Yongbo Yang
    • 1
  • Kaiyu Liu
    • 1
  • Peter Tijssen
    • 3
  • Jianxin Peng
    • 1
    Email author
  • Yi Li
    • 1
    • 2
    Email author
  1. 1.College of Life Sciences, Central China Normal UniversityWuhanPeople’s Republic of China
  2. 2.Bioengineering DepartmentWuhan Bioengineering InstituteWuhanPeople’s Republic of China
  3. 3.INRS-Institut Armand FrappierLavalCanada

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