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Virus Genes

, Volume 46, Issue 1, pp 105–110 | Cite as

Sequencing of two Sunflower chlorotic mottle virus isolates obtained from different natural hosts shed light on its evolutionary history

  • N. Bejerman
  • F. Giolitti
  • S. de Breuil
  • S. Lenardon
Article

Abstract

Sunflower chlorotic mottle virus (SuCMoV), the most prevalent virus of sunflower in Argentina, was reported naturally infecting not only sunflower but also weeds. To understand SuCMoV evolution and improve the knowledge on its variability, the complete genomic sequences of two SuCMoV isolates collected from Dipsacus fullonum (-dip) and Ibicella lutea (-ibi) were determined from three overlapping cDNA clones and subjected to phylogenetic and recombination analyses. SuCMoV-dip and -ibi genomes were 9,953-nucleotides (nt) long; their sequences contained an open reading frame of 9,561 nucleotides, which encoded a polyprotein of 3,187 amino acids flanked by a 5′-noncoding region (NCR) of 135 nt and a 3′-NCR of 257 nt. SuCMoV-dip and -ibi genome nucleotide sequences were 90.9 identical and displayed 90 and 94.6 % identity to that of SuCMoV-C, and 90.8 and 91.4 % identity to that of SuCMoV-CRS, respectively. P1 of SuCMoV-dip and -ibi was 3-nt longer than that of SuCMoV-CRS, but 12-nt shorter than that of SuCMoV-C. Two recombination events were detected in SuCMoV genome and the analysis of dN/dS ratio among SuCMoV complete sequences showed that the genomic regions are under different evolutionary constraints, suggesting that SuCMoV evolution would be conservative. Our findings provide evidence that mutation and recombination would have played important roles in the evolutionary history of SuCMoV.

Keywords

Potyvirus Complete genomes Phylogenetic analysis Evolution SuCMoV 

Notes

Acknowledgments

We thank INTA for financial support. We are grateful to Dr. E. Taleisnik for editing the manuscript and to Dr. K. Ohshima and Dr. M. Adams for critical reading of the manuscript.

Supplementary material

11262_2012_817_MOESM1_ESM.doc (39 kb)
Supplementary material 1 (DOC 39 kb)
11262_2012_817_MOESM2_ESM.doc (29 kb)
Supplementary material 2 (DOC 29 kb)

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • N. Bejerman
    • 1
  • F. Giolitti
    • 1
  • S. de Breuil
    • 1
  • S. Lenardon
    • 1
  1. 1.Instituto de Patología Vegetal (IPAVE), Centro de Investigaciones Agropecuarias (CIAP)Instituto Nacional de Tecnología Agropecuaria (INTA)CórdobaArgentina

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