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Virus Genes

, Volume 45, Issue 1, pp 98–104 | Cite as

Identification and phylogenetic analysis of orf viruses isolated from outbreaks in goats of Assam, a northeastern state of India

  • Durlav Prasad BoraEmail author
  • Nagendra Nath Barman
  • Sailendra Kumar Das
  • Veerakyathappa Bhanuprakash
  • Revanaiah Yogisharadhya
  • Gnanavel Venkatesan
  • Amit Kumar
  • Gitika Rajbongshi
  • Elina Khatoon
  • Apurba Chakraborty
  • Kamal Malla Bujarbaruah
Article

Abstract

Two outbreaks of orf virus (ORFV) (a parapoxvirus) infection in goats, which occurred in Golaghat and Kamrup districts of Assam, a northeastern part of India, were investigated. The disease was diagnosed by standard virological and molecular techniques. The entire protein-coding region of B2L gene of two isolates were cloned and sequenced. Phylogenetic analysis based on B2L amino acid sequences showed that the ORFVs identified in these outbreaks were closely related to each other and both were closer to ORFV-Shahjahanpur 82/04 isolate from north India. The present study revealed that the precise characterization of the genomic region (B2L gene) might provide evidence for the genetic variation and movement of circulating ORFV strains in India.

Keywords

B2L gene Contagious ecthyma Goat Orf virus Phylogenetic analysis Northeast India 

Notes

Acknowledgments

The authors are thankful to the Director, Indian Veterinary Research Institute (IVRI), Izatnagar, and the Head, Division of Virology, IVRI, Mukteswar, Uttarakhand, for providing the necessary facilities. The authors thank the Head of Department, Microbiology, College of Veterinary Science, Khanapara, and field veterinarians for their kind cooperation in providing clinical samples, photographs, and epidemiological information of contagious ecthyma outbreaks.

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Durlav Prasad Bora
    • 1
    • 2
    Email author
  • Nagendra Nath Barman
    • 2
  • Sailendra Kumar Das
    • 2
  • Veerakyathappa Bhanuprakash
    • 1
  • Revanaiah Yogisharadhya
    • 1
  • Gnanavel Venkatesan
    • 1
  • Amit Kumar
    • 1
  • Gitika Rajbongshi
    • 2
  • Elina Khatoon
    • 2
  • Apurba Chakraborty
    • 3
  • Kamal Malla Bujarbaruah
    • 4
  1. 1.Pox Virus Laboratory, Division of VirologyIndian Veterinary Research InstituteKumaonIndia
  2. 2.Department of Microbiology, College of Veterinary ScienceAssam Agricultural UniversityGuwahatiIndia
  3. 3.AAUGuwahatiIndia
  4. 4.Assam Agriculture UniversityJorhatIndia

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