Virus Genes

, Volume 44, Issue 3, pp 539–547 | Cite as

Identification of a new Bombyx mori nucleopolyhedrovirus and analysis of its bro gene family

  • Jing-Bo Zhou
  • Xing-Qi Li
  • Wanchai De-Eknamkul
  • Siripuk Suraporn
  • Jia-Ping Xu


The highly pathogenic Bombyx mori nucleopolyhedrovirus (BmNPV) has caused severe damages to sericulture in many countries, and the relationship between the pathogenicity of various BmNPV strains and their geographical evolution has been the topic of our interest. In this study, we isolated a new BmNPV strain from Thailand (BmNPV-Thai), based on the sequences of its conservative genes p10, p35, polh, egt and vp39. The BmNPV-Thai appears to have baculovirus repeated ORF (bro) genes different from four other well-known BmNPV strains of China (GD, CQ1), Japan (T3), and France (SC7); It only has bro-a, bro-c, and bro-d, but not bro-b and bro-e genes. These bro genes are localized only in the two subgroups highly homologous to their counterparts and their encoded BRO proteins differ mainly at their N-terminal amino acid residues. Phylogenetic analysis indicates that the evolution of the bro genes of the five BmNPV strains is not obviously associated with their geographic locations.


Bombyx mori nucleopolyhedrovirus Strain Thai p10 p35 polh egt vp39 bro Identification 



We greatly acknowledge the National 863 plans projects of China (2011AA100306), the National Natural Sciences Foundation of China (No. 10975002), Sino-Thai Scientific and Cooperation project (19-501J), TICA and Thailand Office of Higher Education Commission.


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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Jing-Bo Zhou
    • 1
  • Xing-Qi Li
    • 1
  • Wanchai De-Eknamkul
    • 2
  • Siripuk Suraporn
    • 3
  • Jia-Ping Xu
    • 1
  1. 1.Department of Life SciencesAnhui Agricultural UniversityHefeiPeople’s Republic of China
  2. 2.Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences and Research Center for Biodiversity ManagementChulalongkorn UniversityBangkokThailand
  3. 3.Department of Biology, Faculty of ScienceMahasarakham University, Khamriang CampusKantravichai DistrictThailand

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