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Virus Genes

, Volume 44, Issue 1, pp 40–44 | Cite as

Detection of bat coronaviruses from Miniopterus fuliginosus in Japan

  • Kazuya Shirato
  • Ken Maeda
  • Shumpei Tsuda
  • Kazuo Suzuki
  • Shumpei Watanabe
  • Hiroshi Shimoda
  • Naoya Ueda
  • Koichiro Iha
  • Satoshi Taniguchi
  • Shigeru Kyuwa
  • Daiji Endoh
  • Shutoku Matsuyama
  • Ichiro Kurane
  • Masayuki Saijo
  • Shigeru Morikawa
  • Yasuhiro Yoshikawa
  • Hiroomi Akashi
  • Tetsuya MizutaniEmail author
Article

Abstract

Bats have great potential as reservoirs for emerging viruses such as severe acute respiratory syndrome-coronavirus. In this study, bat coronaviruses (BtCoVs) were detected by RT-PCR from intestinal and fecal specimens of Miniopterus fuliginosus breeding colonies in Wakayama Prefecture caves, where we previously identified bat betaherpesvirus 2. Two primer sets were used for the detection of BtCoV: one was for the RNA-dependent RNA polymerase (RdRp) region and the other was for the spike (S) protein region. Eleven and 73% of intestinal and fecal specimens, respectively, were positive for RdRp region, and 2 and 40% of those were positive for S protein region. Sequencing and phylogenetic analysis showed that the detected BtCoV belonged to the group 1 (alpha) coronaviruses. These data suggest that BtCoV is endemic in M. fuliginosus in Japan.

Keywords

Bats Coronavirus Miniopterus fuliginosus Virus detection 

Notes

Acknowledgments

We thank Mrs. Momoko Ogata and Miyuki Kawase for their assistance. This study was supported in part by a Grant from the Japan Society for the Promotion of Science, by the Ministry of Health, Labour, and Welfare, Japan.

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Kazuya Shirato
    • 1
  • Ken Maeda
    • 2
  • Shumpei Tsuda
    • 3
  • Kazuo Suzuki
    • 4
  • Shumpei Watanabe
    • 3
  • Hiroshi Shimoda
    • 2
  • Naoya Ueda
    • 5
  • Koichiro Iha
    • 5
  • Satoshi Taniguchi
    • 5
  • Shigeru Kyuwa
    • 5
  • Daiji Endoh
    • 6
  • Shutoku Matsuyama
    • 1
  • Ichiro Kurane
    • 7
  • Masayuki Saijo
    • 7
  • Shigeru Morikawa
    • 7
  • Yasuhiro Yoshikawa
    • 5
  • Hiroomi Akashi
    • 3
  • Tetsuya Mizutani
    • 7
    Email author
  1. 1.Department of Virology 3National Institute of Infectious DiseasesMusashimurayamaJapan
  2. 2.Laboratory of Veterinary Microbiology, Faculty of AgricultureYamaguchi UniversityYamaguchiJapan
  3. 3.Department of Veterinary Microbiology, Graduate School of Agricultural and Life SciencesThe University of TokyoBunkyo-kuJapan
  4. 4.Hikiiwa Park CenterTanabeJapan
  5. 5.Department of Biomedical Science, Graduate School of Agricultural and Life SciencesThe University of TokyoBunkyo-kuJapan
  6. 6.Department of Veterinary Radiology, School of Veterinary MedicineRakuno Gakuen UniversityEbetsuJapan
  7. 7.Department of Virology 1National Institute of Infectious DiseasesMusashimurayamaJapan

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