Virus Genes

, Volume 43, Issue 1, pp 1–5

Brief report: molecular characterization of a novel reassorted pandemic H1N1 2009 in Thai pigs

  • Pravina Kitikoon
  • Donruethai Sreta
  • Suparlark Nuntawan Na Ayudhya
  • Manoosak Wongphatcharachai
  • Jiradej Lapkuntod
  • Duangduean Prakairungnamthip
  • Napawan Bunpapong
  • Sanipa Suradhat
  • Roongroje Thanawongnuwech
  • Alongkorn Amonsin
Article

Abstract

For the past 10 years, endemic swine influenza H1 viruses in Thailand have been characterized as reassortants of swine virus genes from swine influenza viruses (SIV) in US and European pigs. Here the authors report the emergence of a novel reassorted H1N1 (rH1N1) virus consisted of human, avian, and swine virus genes from the pandemic H1N1 2009 (pH1N1) virus with a neuraminidase (NA) gene from a Thai swine H1N1 (ThH1N1) isolate. The rH1N1 virus was detected in nursery pigs during a respiratory disease outbreak in central Thailand in early 2010. The rH1N1 virus was repeatedly isolated from infected pigs, suggesting that it can transmit efficiently among the pig population. The appearance of rH1N1 virus in the field occurred within months of the introduction of pH1N1 virus into the Thai swine population in late 2009. The finding highlights the role of pig in generating newly reassorted influenza A viruses and also the significance of continuing disease surveillance and genetic characterization of SIV in pigs.

Keywords

Influenza Pandemic H1N1 2009 Swine Reassort Thailand 

Supplementary material

11262_2011_597_MOESM1_ESM.ppt (61 kb)
Supplementary Fig. 1. Hemagglutinin-inhibition (HI) titers of pigs from different age groups to pH1N1, rH1N1 and ThH1N1 (PPT 61 kb)

References

  1. 1.
    F.S. Dawood, S. Jain, L. Finelli, M.W. Shaw, S. Lindstrom, R.J. Garten, L.V. Gubareva, X. Xu, C.B. Bridges, T.M. Uyeki, N. Engl. J. Med. 360, 2605–2615 (2009)PubMedCrossRefGoogle Scholar
  2. 2.
    G.J. Smith, D. Vijaykrishna, J. Bahl, S.J. Lycett, M. Worobey, O.G. Pybus, S.K. Ma, C.L. Cheung, J. Raghwani, S. Bhatt, J.S. Peiris, Y. Guan, A. Rambaut, Nature 459, 1122–1125 (2009)PubMedCrossRefGoogle Scholar
  3. 3.
    C. Kingsford, N. Nagarajan, S.L. Salzberg, PLoS One 4, e6402 (2009)Google Scholar
  4. 4.
    A. Pereda, J. Cappuccio, M.A. Quiroga, E. Baumeister, L. Insarralde, M. Ibar, R. Sanguinetti, M.L. Cannilla, D. Franzese, O.E. Escobar Cabrera, M.I. Craig, A. Rimondi, M. Machuca, R.T. Debenedetti, C. Zenobi, L. Barral, R. Balzano, S. Capalbo, A. Risso, C.J. Perfumo, Emerg. Infect. Dis. 16, 304–307 (2010)PubMedGoogle Scholar
  5. 5.
    M.S. Song, J.H. Lee, P.N. Pascua, Y.H. Baek, H.I. Kwon, K.J. Park, H.W. Choi, Y.K. Shin, J.Y. Song, C.J. Kim, Y.K. Choi, J. Clin. Microbiol. 48, 3204–3211 (2010)PubMedCrossRefGoogle Scholar
  6. 6.
    D. Sreta, S. Tantawet, S.N. Na Ayudhya, A. Thontiravong, M. Wongphatcharachai, J. Lapkuntod, N. Bunpapong, R. Tuanudom, S. Suradhat, L. Vimolket, Y. Poovorawan, R. Thanawongnuwech, A. Amonsin, P. Kitikoon, Emerg. Infect. Dis. 16, 1587–1590 (2010)PubMedGoogle Scholar
  7. 7.
    H.M. Weingartl, Y. Berhane, T. Hisanaga, J. Neufeld, H. Kehler, C. Emburry-Hyatt, K. Hooper-McGreevy, S. Kasloff, B. Dalman, J. Bystrom, S. Alexandersen, Y. Li, J. Pasick, J. Virol. 84, 2245–2256 (2010)PubMedCrossRefGoogle Scholar
  8. 8.
    D. Vijaykrishna, L.L. Poon, H.C. Zhu, S.K. Ma, O.T. Li, C.L. Cheung, G.J. Smith, J.S. Peiris, Y. Guan, Science 328, 1529 (2010)PubMedCrossRefGoogle Scholar
  9. 9.
    E. Starick, E. Lange, S. Fereidouni, C. Bunzenthal, R. Hoveler, A. Kuczka, E. Grosse Beilage, H.P. Hamann, I. Klingelhofer, D. Steinhauer, T. Vahlenkamp, M. Beer, and T. Harder, J. Gen. Virol. (2011) [Epub ahead of print]Google Scholar
  10. 10.
    P. Kitikoon, D. Nilubol, B.J. Erickson, B.H. Janke, T.C. Hoover, S.A. Sornsen, E.L. Thacker, Vet. Immunol. Immunopathol. 112, 117–128 (2006)PubMedCrossRefGoogle Scholar
  11. 11.
    S. Al Faress, O. Ferraris, V. Moules, M. Valette, A. Hay, B. Lina, Virus Res. 132, 33–41 (2008)PubMedCrossRefGoogle Scholar
  12. 12.
    N. Komadina, V. Roque, P. Thawatsupha, J. Rimando-Magalong, S. Waicharoen, E. Bomasang, P. Sawanpanyalert, M. Rivera, P. Iannello, A.C. Hurt, I.G. Barr, Virus Genes 35, 161–165 (2007)PubMedCrossRefGoogle Scholar
  13. 13.
    A.L. Vincent, W. Ma, K.M. Lager, B.H. Janke, J.A. Richt, Adv. Virus Res. 72, 127–154 (2008)PubMedCrossRefGoogle Scholar
  14. 14.
    R. Zell, S. Bergmann, A. Krumbholz, P. Wutzler, R. Durrwald, Arch. Virol. 153, 2085–2092 (2008)PubMedCrossRefGoogle Scholar
  15. 15.
    N. Takemae, S. Parchariyanon, S. Damrongwatanapokin, Y. Uchida, R. Ruttanapumma, C. Watanabe, S. Yamaguchi, T. Saito, Influenza Other Respi Viruses 2, 181–189 (2008)PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Pravina Kitikoon
    • 1
    • 2
  • Donruethai Sreta
    • 2
  • Suparlark Nuntawan Na Ayudhya
    • 2
  • Manoosak Wongphatcharachai
    • 3
  • Jiradej Lapkuntod
    • 1
    • 3
  • Duangduean Prakairungnamthip
    • 1
    • 2
  • Napawan Bunpapong
    • 1
  • Sanipa Suradhat
    • 1
    • 4
  • Roongroje Thanawongnuwech
    • 1
    • 2
  • Alongkorn Amonsin
    • 1
    • 3
  1. 1.Emerging and Re-emerging Infectious Diseases in Animals, Research UnitFaculty of Veterinary Science, Chulalongkorn UniversityBangkokThailand
  2. 2.Department of PathologyFaculty of Veterinary Science, Chulalongkorn UniversityBangkokThailand
  3. 3.Department of Veterinary Public HealthFaculty of Veterinary Science, Chulalongkorn UniversityBangkokThailand
  4. 4.Department of Microbiology, Faculty of Veterinary Science Chulalongkorn UniversityBangkokThailand

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