Virus Genes

, Volume 42, Issue 3, pp 388–393 | Cite as

Foot-and-mouth disease virus leader proteinase inhibits dsRNA-induced RANTES transcription in PK-15 cells

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Abstract

The chemokine RANTES (regulated upon activation, normal T-cells expressed and secreted) plays an essential role in inflammation and immune response. Infection with wild-type foot-and-mouth disease virus (FMDV) in PK-15 cells strongly inhibits the expression of RANTES compared to infection with a genetically engineered mutant lacking the leader protein (Lpro) coding region. This suggests that Lpro is involved in RANTES regulation. However, the underlying molecular mechanism remains unclear. In this study, we show that transfection of PK-15 cells with a plasmid expressing the Lpro of FMDV, in the absence of other FMDV proteins, inhibited dsRNA-induced RANTES transcription and promoter activity. Promoter mutagenesis experiments revealed that the interferon-stimulated response element (ISRE) was important for the ability of Lpro to inhibit dsRNA-induced RANTES promoter activity. Furthermore, over-expression of Lpro also inhibited IRF-3/7-mediated RANTES activation. Screening Lpro mutants indicated that catalytic activity and a SAP (for SAF-A/B, Acinus, and PIAS) domain of Lpro were required to suppress dsRNA-induced RANTES transcription.

Keywords

FMDV Leader proteinase RANTES Inhibition Signaling pathway 
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Notes

Acknowledgments

We thank Dr. Kone Yedamignou Alexis for critical review of the manuscript. This study was supported by the Program for the New Century Excellent Talent Project (NCET-07-0347) and Changjiang Scholars and Innovative Research Team in Chinese University (IRT0726).

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Dang Wang
    • 1
  • Liurong Fang
    • 1
    • 2
  • Jing Bi
    • 1
  • Quangang Chen
    • 1
  • Lu Cao
    • 1
  • Rui Luo
    • 1
  • Huanchun Chen
    • 1
  • Shaobo Xiao
    • 1
    • 2
  1. 1.Division of Animal Infectious Diseases, State Key Laboratory of Agricultural Microbiology, College of Veterinary MedicineHuazhong Agricultural UniversityWuhanPeople’s Republic of China
  2. 2.Laboratory of Animal Virology, College of Veterinary MedicineHuazhong Agricultural UniversityWuhanPeople’s Republic of China

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