The genome of Oryctes rhinoceros nudivirus provides novel insight into the evolution of nuclear arthropod-specific large circular double-stranded DNA viruses
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The Oryctes rhinoceros nudivirus (OrNV) is a dsDNA virus with enveloped, rod-shaped virions. Its genome is 127,615 bp in size and contains 139 predicted protein-coding open reading frames (ORFs). In-depth genome sequence comparisons revealed a varying number of shared gene homologues, not only with other nudiviruses (NVs) and baculoviruses, but also with other arthropod-specific large dsDNA viruses, including the so-called Monodon baculovirus (MBV), the salivary gland hypertrophy viruses (SGHVs) and white spot syndrome virus (WSSV). Nudivirus genomes contain 20 baculovirus core gene homologues associated with transcription (p47, lef-8, lef-9, lef-4, vlf-1, and lef-5), replication (dnapol and helicase), virus structure (p74, pif-1, pif-2, pif-3, 19kda/pif-4, odv-e56/pif-5, vp91, vp39, and 38K), and unknown functions (ac68, ac81, and p33). Most strikingly, a set of homologous genes involved in peroral infection (p74, pif-1, pif-2, and pif-3) are common to baculoviruses, nudiviruses, SGHVs, and WSSV indicating an ancestral mode of infection in these highly diverged viruses. A gene similar to polyhedrin/granulin encoding the baculovirus occlusion body protein was identified in non-occluded NVs and in Musca domestica SGHV evoking the question of the evolutionary origin of the baculovirus polyhedrin/granulin gene. Based on gene homologies, we further propose that the shrimp MBV is an occluded member of the nudiviruses. We conclude that baculoviruses, NVs and the shrimp MBV, the SGHVs and WSSV share the significant number of conserved genetic functions, which may point to a common ancestry of these viruses.
KeywordsOryctes rhinoceros nudivirus Genome Monodon baculovirus Salivary gland hypertrophy viruses White spot syndrome virus Peroral infection Ancestry
This work was supported by a grant of the Deutsche Forschungsgemeinschaft (Je245-7) to J.A.J. M.v.O. was supported by a MEERVOUD grant from the Research Council of Earth and Life Sciences (ALW) with financial aid from the Netherlands Organization for Scientific Research (NWO) (Project 836.05.070). Dwight Lynn is acknowledged for helpful comments on the manuscript.
- 11.J.M. Vlak, J.R. Bonami, T.W. Flegel, G.H. Kou, D.V. Lightner, C.F. Lo, P.C. Loh, P.W. Walker, in Virus Taxonomy: The Eighth Report of the International Committee on Taxonomy of Viruses, ed. by C.M. Fauquet, M.A. Mayo, J. Maniloff, U. Desselberger, L.A. Ball (Elsevier Academic Press, New York, 2005), pp. 187–192Google Scholar
- 19.A. Marchler-Bauer, J.B. Anderson, M.K. Derbyshire, C. DeWeese-Scott, N.R. Gonzales, M. Gwadz, L. Hao, S. He, D.I. Hurwitz, J.D. Jackson, Z. Ke, D. Krylov, C.J. Lanczycki, C.A. Liebert, C. Liu, F. Lu, S. Lu, G.H. Marchler, M. Mullokandov, J.S. Song, N. Thanki, R.A. Yamashita, J.J. Yin, D. Zhang, S.H. Bryant, Nucleic Acids Res. 35, D237–D240 (2007)CrossRefGoogle Scholar
- 27.D.A. Theilmann, G.W. Blissard, B. Bonning, J.A. Jehle, D.R. O’Reilly, G.F. Rohrmann, S. Thiem, J.M. Vlak, in Virus Taxonomy: The Eighth Report of the International Committee on Taxonomy of Viruses, ed. C.M. Fauquet, M.A. Mayo, J. Maniloff, U. Desselberger, L.A. Ball (Elsevier Academic Press, New York 2005), pp. 177–185Google Scholar
- 51.A.M. Huger, A. Krieg, in Atlas of Invertebrate Viruses, ed. by J.R. Adams, J.R. Bonami (CRC Press, Inc., Boca Raton, 1991), pp. 287–319Google Scholar