Phylogenetic analysis of the E2 gene of classical swine fever virus from the Guangxi Province of southern China
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In this study, suspected classical swine fever (CSF) samples from the Guangxi Province of China were obtained from pigs with acute CSF, aborted fetuses, newborn pigs that died at 1–2 days of age, tonsils of healthy pigs, and leukocytes of immunized sows during 2001–2009. About 92 of 775 samples were found to be positive by RT-PCR, and 41 isolates were obtained. Phylogenetic analysis was performed on the 31 isolates by sequencing the E2 gene, and the isolates were found to cluster into two groups: (1) isolates from aborted fetuses (except GXGZ02), deceased newborn baby pigs, tonsils of healthy pigs, and leukocytes of immunized sows belonged to group 1.1, along with vaccine strain, HCLV, and standard virulent strain, Shimen, of China, and (2) 20 isolates from pigs with acute CSF belonged to group 2.1, 13 of which were clustered into subgroup 2.1b with isolates from other provinces of China, and 7 of which were clustered into subgroup 2.1a with isolates from Italy and Germany.
KeywordsClassical swine fever virus E2 gene Phylogenetic analysis
This work was supported by National High-Tech R and D Program (863 Program, Grant No. 2007AA10Z160), National Natural Science Fund (Grant No. 31060336), and Guangxi University Key Program for Science and Technology Research (Grant No. 2003ZD01).
Conflicts of interest
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