Activation of NF-κB by nucleocapsid protein of the porcine reproductive and respiratory syndrome virus
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Nuclear factor kappa B (NF-κB) is a critical transcription factor in innate and adaptive immune response as well as cell proliferation and survival. Previous studies have demonstrated that porcine reproductive and respiratory syndrome virus (PRRSV) infection activated NF-κB pathways through IκB degradation in MARC-145 cells and alveolar macrophages. To evaluate the mechanisms behind this, we investigated the role of PRRSV structural proteins in the regulation of NF-κB. In this study, we screened the structural proteins of PRRSV by NF-κB DNA-binding assay and luciferase activity assay and demonstrated that PRRSV nucleocapsid (N) protein could activate NF-κB in MARC-145 cells. Furthermore, we revealed that the region between aa 30 and 73 of N protein was essential for its function in the activation of NF-κB. These results presented here provide a basis for understanding molecular mechanism of PRRSV infection and inflammation response.
KeywordsPorcine reproductive and respiratory syndrome virus (PRRSV) NF-κB N protein
This work was supported by the National Natural Sciences Foundation of China (31001054, 30800046) and the New Century Excellent Talent Project (NCET-07-0347).
- 1.E.J. Neumann, J.B. Kliebenstein, C.D. Johnson, J.W. Mabry, E.J. Bush, A.H. Seitzinger, A.L. Green, J.J. Zimmerman, Assessment of the economic impact of porcine reproductive and respiratory syndrome on swine production in the United States. J. Am. Vet. Med. Assoc. 227, 385–392 (2005)PubMedCrossRefGoogle Scholar
- 16.T. Ait-Ali, A.D. Wilson, D.G. Westcott, M. Clapperton, M. Waterfall, M.A. Mellencamp, T.W. Drew, S.C. Bishop, A.L. Archibald, Innate immune responses to replication of porcine reproductive and respiratory syndrome virus in isolated Swine alveolar macrophages. Viral Immunol. 20, 105–118 (2007)PubMedCrossRefGoogle Scholar
- 20.L.H. Thomas, J.S. Friedland, M. Sharland, S. Becker, Respiratory syncytial virus-induced RANTES production from human bronchial epithelial cells is dependent on nuclear factor-kappa B nuclear binding and is inhibited by adenovirus-mediated expression of inhibitor of kappa B alpha. J. Immunol. 161, 1007–1016 (1998)PubMedGoogle Scholar
- 28.M. Luftig, T. Yasui, V. Soni, M.S. Kang, N. Jacobson, E. Cahir-McFarland, B. Seed, E. Kieff, Epstein-Barr virus latent infection membrane protein 1 TRAF-binding site induces NIK/IKK alpha-dependent noncanonical NF-kappaB activation. Proc. Natl Acad. Sci. USA 101, 141–146 (2004)PubMedCrossRefGoogle Scholar