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Divergent molecular variants of Grapevine virus B (GVB) from corky bark (CB)-affected and CB-negative LN33 hybrid grapevines

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Analysis of two Grapevine virus B (GVB)-infected LN33 hybrid grapevines revealed that a plant exhibiting clear symptoms of corky bark (CB) disease was infected with two molecular variants of the virus, whereas a plant exhibiting no disease symptoms was infected with only one variant. Sequence results indicated that the single variant in the CB-negative grapevine was also one of the two present in the CB-affected hybrid. Plant extracts from these two grapevines were used to successfully transmit the virus to N. benthamiana. After further cloning and sequencing, two clearly divergent variants were identified. Comparative molecular analysis of the variants, named here GVB 953-1 and GVB-H1, respectively, transmitted from CB-affected and consistently CB-negative plants, revealed short genomic regions, most of them highly divergent, that encoded amino acid sequences, containing significant amino acid substitutions altering the net charges of their respective proteins. Interestingly, a comparison of these variants to genome sequence data of GVB variants GVB Italy and GVB 94/971 available from the GenBank, revealed that these significant amino acid substitutions were the same for, and unique to, the variant pairs GVB 953-1/GVB Italy and GVB-H1/GVB 94/971. This despite the variants of each pair being otherwise clearly different at nucleotide and amino acid levels. In addition, both sets of variants differed substantially in their respective 3′-non-translated (3′NTR) regions. The relevance of these findings is discussed.

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  1. 1.

    E.F. Beukman, E.M. Gifford, Hilgardia 4, 73–103 (1969)

  2. 2.

    E.F. Beukman, A.C. Goheen, in Virus Diseases of Small Fruits and Grapevines, ed. by N.W. Frazier (University of California, Berkeley, 1970), pp. 207–209

  3. 3.

    D. Boscia, V. Savino, A. Minafra, S. Namba, V. Elicio, M.A. Castellano, D. Gonsalves, G.P. Martelli, Arch. Virol. 130, 109–120 (1993)

  4. 4.

    D. Boscia, N. Abu-Ghanem, P. Sardarelli, A. Minafra, M.A. Castellano, R. Garau, V. Savino, G.P. Martelli, Riv. Pat. Veg. SV 4, 11–24 (1994)

  5. 5.

    D. Boscia, A. Minafra, G.P. Martelli, in Filamentous Viruses of Woody Plants, ed. by P.L. Monette (Research Signpost, Trivandrum, 1997), pp. 19–28

  6. 6.

    C.I. Dovas, N.I. Katis, J. Virol. Methods 170, 99–106 (2003)

  7. 7.

    D.J. Engelbrecht, G.G.F. Kasdorf, Phytophylactica 22, 347–354 (1990)

  8. 8.

    D.E. Goszczynski, Plant. Pathol. 56, 755–762 (2007)

  9. 9.

    D.E. Goszczynski, A.E.C. Jooste, Vitis 41, 77–82 (2002)

  10. 10.

    D.E. Goszczynski, G.G.F. Kasdorf, G. Pietersen, J. Phytopathol. 144, 581–583 (1996)

  11. 11.

    D.E. Goszczynski, J. du Preez, J.T. Burger, Virus Res. 138, 105–110 (2008)

  12. 12.

    N. Habili, R.H. Symons, Aust. Grapegrow. Winemak. 429, 58–59 (1999)

  13. 13.

    W.B. Hewitt, Bull. Calif. Dep. Agric. 43, 47–64 (1954)

  14. 14.

    W.B. Hewitt, A.C. Goheen, D.J. Raski, G.V. Gooding, Vitis 3, 57–83 (1962)

  15. 15.

    J.S. Hu, D. Gonsalves, D. Teliz, J. Phytopathol. 128, 133–135 (1990)

  16. 16.

    M. Kimura, J. Mol. Evol. 16, 111–120 (1980)

  17. 17.

    G.P. Martelli, E. Boudon-Padieu, in Directory of Infectious Diseases of Grapevines and Viruses and Virus-Like Diseases of the Grapevine: Bibliographic Report 1998–2004, Bari, Italy, 2006

  18. 18.

    G.P. Martelli, A. Minafra, P. Sardarelli, Arch. Virol. 142, 1929–1932 (1997)

  19. 19.

    Y. Moskovitz, D.E. Goszczynski, L. Bir, A. Fingstein, H. Czosnek, M. Mawassi, Arch. Virol. 153, 323–328 (2008)

  20. 20.

    N. Saitou, M. Nei, Mol. Biol. Evol. 4, 406–425 (1987)

  21. 21.

    P. Sardarelli, A. Minafra, G.P. Martelli, J. Gen. Virol. 77, 2645–2652 (1996)

  22. 22.

    B.-J. Shi, N. Habili, R. Gafny, R.H. Symons, Virus Genes 29, 279–285 (2004)

  23. 23.

    M. Whattam, Aust. Plant Pathol. 30, 379–380 (2001)

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The author thanks Winetech, South Africa, for partially funding this study.

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Correspondence to Dariusz E. Goszczynski.

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Goszczynski, D.E. Divergent molecular variants of Grapevine virus B (GVB) from corky bark (CB)-affected and CB-negative LN33 hybrid grapevines. Virus Genes 41, 273–281 (2010). https://doi.org/10.1007/s11262-010-0511-6

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  • Grapevine virus B variants
  • LN33 hybrid grapevine
  • Corky bark disease