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Virus Genes

, Volume 41, Issue 2, pp 149–157 | Cite as

The pleiotropic protein kinase CK2 phosphorylates HTLV-1 Tax protein in vitro, targeting its PDZ-binding motif

  • Carlo BidoiaEmail author
  • Marco Mazzorana
  • Mario A. Pagano
  • Giorgio Arrigoni
  • Flavio Meggio
  • Lorenzo A. Pinna
  • Umberto Bertazzoni
Article

Abstract

The HTLV-1 transactivator Tax is an oncoprotein capable of deregulating the expression of many cellular genes and interfering with signalling pathways. Here we show that Tax-1 is phosphorylated in vitro by the pleiotropic human serine/threonine kinase CK2 at three residues, Ser-336, Ser-344 and Thr-351, close to and within its C-terminal PDZ-binding motif. We also show that the mutation of Thr-351 to aspartate abolishes Tax-1 binding to the scaffold protein hDlg, a tumour suppressor factor, while having no effect on transactivation. These results suggest that CK2, whose constitutive activity is often hijacked by viruses to sustain their vital cycle, could modulate Tax-1 oncogenic interactions.

Keywords

CK2 Tax-1 HTLV PDZ PDZ-binding motif PBM 

Notes

Acknowledgements

This work was supported in part by the following grants to Umberto Bertazzoni: MIUR PRIN 2007, Fondazione Cariverona 2008 and AIRC Regional Program 2008. The authors express their thanks to Prof. Lucy Rasmussen (Stanford University) for a critical reading of the paper, to Lawrence Banks (International Centre for Genetic Engineering and Biotechnology, Trieste, Italy) for the plasmid expressing HA-hDlg, and to Françoise Bex (Université Libre de Bruxelles, Bruxelles, Belgium) for the plasmid containing the cDNA of Tax-1.

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Carlo Bidoia
    • 1
    • 5
    Email author
  • Marco Mazzorana
    • 2
    • 3
  • Mario A. Pagano
    • 4
  • Giorgio Arrigoni
    • 4
  • Flavio Meggio
    • 4
  • Lorenzo A. Pinna
    • 2
    • 4
  • Umberto Bertazzoni
    • 1
  1. 1.Department of Life and Reproduction Sciences, Section of Biology and GeneticsUniversity of VeronaVeronaItaly
  2. 2.Venetian Institute for Molecular MedicinePadovaItaly
  3. 3.Structural Biology and Biocomputing Programme CNIO - Centro Nacional de Investigaciones OncológicasMadridSpain
  4. 4.Department of Biological ChemistryUniversity of PaduaPadovaItaly
  5. 5.Centre for Research in Infectious Diseases, School of Medicine and Medical ScienceUniversity College DublinBelfield, Dublin 4Ireland

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