Identification and molecular characterization of begomovirus and associated satellite DNA molecules infecting Cyamopsis tetragonoloba
Monopartite begomoviruses comprise DNA-A as the main genome and associated satellite DNAs. Viral DNA extracted from guar (Cyamopsis tetragonoloba) showing leaf curl symptoms exhibited positive amplification of coat protein (CP) gene of DNA-A component, suggesting the presence of begomovirus. Full length DNA-A was amplified by primer pair re-designed from CP gene nucleotide sequence. The associated alphasatellite and betasatellite DNA molecules were amplified and sequenced, confirming the presence of monopartite begomovirus. Sequence comparisons showed 89% identity with other begomoviruses. The Neighbor-Joining tree based on full length DNA-A nucleotide sequence showed that the guar infecting begomovirus clustered separately from other known begomoviruses. The betasatellite shared a high (96%) nucleotide identity to Cotton leaf curl Multan betasatellites. The alphasatellite showed 91% nucleotide identity to alphasatellite associated with begomovirus infecting Okra. Recombination analyses showed three recombinant fragments in DNA-A, two in betasatellite, and four in alphasatellite. The results suggest that the begomovirus identified in this study was a new recombinant virus. Its name was proposed as Cyamopsis tetragonoloba leaf curl virus (CyTLCuV).
KeywordsBegomovirus Cyamopsis tetragonoloba leaf curl virus Coat protein gene DNA-A Alphasatellite Betasatellite
- 1.J. Stanley, D.M. Bisaro, R.W. Briddon, J.K. Brown, C.M. Fauquet, B.D. Harrison, E.P. Rybicki, D.C. Stenger, in Virus Taxonomy, ed. by C.M. Fauquet, M.A. Mayo, J. Maniloff, U. Desselberger, L.A. Ball. Eighth Report of International Committee on Taxonomy of Viruses (Elsevier, London, 2005), pp. 301–326Google Scholar
- 18.J. Kumar, A. Kumar, J.A. Khan, Aminuddin, J. Plant Pathol. (2010, in press)Google Scholar
- 19.J. Kumar, A. Kumar, S. Gunapati, J.A. Khan, J. Plant Pathol. (2010, in press)Google Scholar
- 22.J.A. Khan, S.S. Sohrab, Aminuddin, R.K. Gupta, J. Plant Dis. Prot. 109, 68–73 (2002)Google Scholar