Virus Genes

, Volume 41, Issue 1, pp 118–125

Identification and molecular characterization of begomovirus and associated satellite DNA molecules infecting Cyamopsis tetragonoloba

  • J. Kumar
  • A. Kumar
  • J. K. Roy
  • R. Tuli
  • J. A. Khan
Article

Abstract

Monopartite begomoviruses comprise DNA-A as the main genome and associated satellite DNAs. Viral DNA extracted from guar (Cyamopsis tetragonoloba) showing leaf curl symptoms exhibited positive amplification of coat protein (CP) gene of DNA-A component, suggesting the presence of begomovirus. Full length DNA-A was amplified by primer pair re-designed from CP gene nucleotide sequence. The associated alphasatellite and betasatellite DNA molecules were amplified and sequenced, confirming the presence of monopartite begomovirus. Sequence comparisons showed 89% identity with other begomoviruses. The Neighbor-Joining tree based on full length DNA-A nucleotide sequence showed that the guar infecting begomovirus clustered separately from other known begomoviruses. The betasatellite shared a high (96%) nucleotide identity to Cotton leaf curl Multan betasatellites. The alphasatellite showed 91% nucleotide identity to alphasatellite associated with begomovirus infecting Okra. Recombination analyses showed three recombinant fragments in DNA-A, two in betasatellite, and four in alphasatellite. The results suggest that the begomovirus identified in this study was a new recombinant virus. Its name was proposed as Cyamopsis tetragonoloba leaf curl virus (CyTLCuV).

Keywords

Begomovirus Cyamopsis tetragonoloba leaf curl virus Coat protein gene DNA-A Alphasatellite Betasatellite 

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • J. Kumar
    • 1
  • A. Kumar
    • 1
  • J. K. Roy
    • 2
  • R. Tuli
    • 3
  • J. A. Khan
    • 1
  1. 1.Molecular Virology LaboratoryNational Botanical Research InstituteLucknowIndia
  2. 2.Plant Molecular Biology LaboratoryNational Botanical Research InstituteLucknowIndia
  3. 3.National Agri-Food Biotechnology InstituteMohaliIndia

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