Advertisement

Virus Genes

, Volume 41, Issue 1, pp 105–110 | Cite as

Molecular characterization of two Chinese isolates of Beet western yellows virus infecting sugar beet

  • Hai-Ying Xiang
  • Shu-Wei Dong
  • Hui-Zhong Zhang
  • Wei-Lin Wang
  • Min-Quan Li
  • Cheng-Gui HanEmail author
  • Da-Wei Li
  • Jia-Lin Yu
Article

Abstract

Beet western yellows virus (BWYV) has previously been reported as an agent of sugar beet yellowing disease in China. In this article, the complete genomic RNA sequences of two Chinese BWYV isolates infecting beet from Inner Mongolia (BWYV-IM) and Gansu (BWYV-GS) were determined and compared with three beet poleroviruses (BMYV, BChV and BWYV-US) and other non-beet-infecting poleroviruses. The genomes of the two isolates were 5,668 nt in length, and had almost the same genomic organization and characteristics as BWYV-US. The full length of BWYV-IM shared nucleotide sequence identities of 97.4, 86.6, 64.4 and 70.8% with BWYV-GS, BWYV-US, BChV and BMYV, respectively. Further sequence analysis indicated that the Chinese BWYV isolates were more closely related to BWYV-US; however, the identity of any gene product between the Chinese isolates and BWYV-US was <90%. Therefore, on the basis of genome sequence, we propose that these Chinese isolates are a distinct strain of BWYV that infect sugar beet. In addition, recombinant detection analysis revealed that BWYV-IM might be a recombinant virus.

Keywords

Beet western yellows virus Chinese isolate Complete genomic sequence Phylogenetic analysis RDP detection 

Notes

Acknowledgements

This work was partially supported by the Natural Science Foundation of China (30671359 and 30471136) and the National Basic Research Program of China (2006CB101903).

References

  1. 1.
    M. Beuve, M. Stevens, H. Liu, W. Wintermantel, S. Hauser, O. Lemaire, Plant Dis. 92, 51–60 (2008)CrossRefGoogle Scholar
  2. 2.
    S. Hauser, M. Stevens, M. Beuve, O. Lemaire, Arch. Virol. 147, 745–762 (2002)CrossRefPubMedGoogle Scholar
  3. 3.
    H. Guilley, K. Richards, G. Jonard, Arch. Virol. 140, 1109–1118 (1995)CrossRefPubMedGoogle Scholar
  4. 4.
    Wintermantel W., Compendium of the Beet Diseases and Insects 2nd edn. (APS Press, St. Paul, 2009), pp. 46–47Google Scholar
  5. 5.
    Duffus J., Phytopathology 54, 1432 (1964)Google Scholar
  6. 6.
    M. Stevens, B. Freeman, H. Liu, E. Herrbach, O. Lemaire, Mol. Plant Pathol. 6, 1–9 (2005)CrossRefGoogle Scholar
  7. 7.
    K. Peter, F. Gildow, P. Palukaitis, S. Gray, J. Virol. 83, 5419–5429 (2009)CrossRefPubMedGoogle Scholar
  8. 8.
    H. Xiang, Q. Shang, C. Han, D. Li, J. Yu, Plant Pathol. 57, 390 (2008)Google Scholar
  9. 9.
    A. Rowhani, R. Stace-Smith, Virology 98, 45–45 (1979)CrossRefPubMedGoogle Scholar
  10. 10.
    C. Han, D. Li, Y. Xing, K. Zhu, Z. Tian, Z. Cai, J. Yu, Y. Liu, Plant Dis. 84, 627–630 (2000)CrossRefGoogle Scholar
  11. 11.
    F. Sanger, S. Nicklen, A. Coulson, Proc. Natl. Acad. Sci. USA 74, 5463–5467 (1977)CrossRefPubMedGoogle Scholar
  12. 12.
    J. Thompson, D. Higgins, T. Gibson, Nucl. Acids Res. 22, 4673–4680 (1994)CrossRefPubMedGoogle Scholar
  13. 13.
    Kumar S., Nei M., Dudley J., Tamura K., Brief. Bioinform. 9, 299–306 (2008)Google Scholar
  14. 14.
    T.A. Hall, Nucl. Acid Symp. Ser. 41, 95–98 (1999)Google Scholar
  15. 15.
    D. Martin, C. Williamson, D. Posada, Bioinformatics 21, 260–262 (2005)CrossRefPubMedGoogle Scholar
  16. 16.
    V. Brault, J. Van den Heuvel, M. Verbeek, V. Ziegler-Graff, A. Reutenauer, E. Herrbach, J. Garaud, H. Guilley, K. Richards, G. Jonard, EMBO J. 14, 650–659 (1995)PubMedGoogle Scholar
  17. 17.
    J. Van den Heuvel, A. Bruyere, S. Hogenhout, V. Ziegler-Graff, V. Brault, M. Verbeek, F. Van Der Wilk, K. Richards, J. Virol. 71, 7258–7265 (1997)PubMedGoogle Scholar
  18. 18.
    A. Bruyere, V. Brault, V. Ziegler-Graff, M. Simonis, J. Van den Heuvel, K. Richards, H. Guilley, G. Jonard, E. Herrbach, Virology 230, 323–334 (1997)CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Hai-Ying Xiang
    • 1
  • Shu-Wei Dong
    • 1
  • Hui-Zhong Zhang
    • 2
  • Wei-Lin Wang
    • 1
  • Min-Quan Li
    • 3
  • Cheng-Gui Han
    • 1
    Email author
  • Da-Wei Li
    • 1
  • Jia-Lin Yu
    • 1
  1. 1.State Key Laboratory for Agrobiotechnology and Agriculture Ministry Laboratory for Plant PathologyChina Agricultural UniversityBeijingChina
  2. 2.Inner Mongolia Academy of Agricultural SciencesHohhotChina
  3. 3.Gansu Agricultural UniversityLanzhouChina

Personalised recommendations