Virus Genes

, 39:249 | Cite as

Molecular characterization and pathogenicity of tomato yellow leaf curl virus in China

  • Hui Zhang
  • Huanran Gong
  • Xueping Zhou


Several tomato production regions in China were surveyed for tomato yellow leaf curl disease (TYLCD), and 31 tomato leaf samples showing TYLCD-like symptoms were collected. The partial or full-length genomes of these isolates were sequenced and tomato yellow leaf curl virus (TYLCV) was detected in Shanghai, Zhejiang, Jiangsu Shandong and Hebei provinces of China. The TYLCV isolates found in China share high sequence identity (>98%) and have more than 97% sequence identity with TYLCV-IL[IL:Reo] (X15656). Phylogenetic relationship analysis reveals that although with little genetic variability, they can form two groups and all the TYLCV isolates in China belong to the group I. An infectious clone of TYLCV-[CN:SH2] (AM282874) was constructed and agro-inoculated into Nicotiana benthamiana, N. tabacum Samsun, N. glutinosa, Solanum lycopersicum, Petunia hybrida, Cucumis sativus, Gossypium hirsutum, S. melongena, and Capsicum annuum. TYLCV-[CN:SH2] can induce severe leaf curling and stunting symptoms in these plants except C. sativus, G. hirsutum, S. melongena and C. annuum. We verified that TYLCV can trans-replicate tomato yellow leaf curl China virus DNA-β in N. benthamiana and S. lycopersicum and induced more severe symptoms with distortion and yellow vein.


Tomato yellow leaf curl virus Sequence Infectivity China 



This work was supported by the National Natural Science Foundation of China (Grants No. 30530520) and the Zhejiang Agricultural Science and Technology Key Research Projects (2007C12054).


  1. 1.
    C.M. Fauquet, R.W. Briddon, J.K. Brown, E. Moriones, J. Stanley, M. Zerbini, X. Zhou, Arch. Virol. 153, 783–821 (2008)CrossRefPubMedGoogle Scholar
  2. 2.
    C.M. Fauquet, D.M. Bisaro, R.W. Briddon, J.K. Brown, B.D. Harrison, E.P. Rybicki, D.C. Stenger, J. Stanley, Arch. Virol. 148, 405–421 (2003)CrossRefPubMedGoogle Scholar
  3. 3.
    A.S. Moffat, Science 286, 1835–1837 (1999)CrossRefGoogle Scholar
  4. 4.
    B.D. Harrison, D.J. Robinson, Annu. Rev. Phytopathol. 37, 369–398 (1999)CrossRefPubMedGoogle Scholar
  5. 5.
    I.B. Dry, J.E. Rigden, L.R. Krake, P.M. Mullineaux, M.A. Rezaian, J. Gen. Virol. 74, 147–151 (1993)CrossRefPubMedGoogle Scholar
  6. 6.
    N. Navot, E. Pichersky, M. Zeidan, D. Zamir, H. Czosnek, Virology 185, 151–161 (1991)CrossRefPubMedGoogle Scholar
  7. 7.
    Y. Antignus, S. Cohen, Phytopathology 84, 707–712 (1994)CrossRefGoogle Scholar
  8. 8.
    H. Czosnek, H. Laterrot, Arch. Virol. 142, 1391–1406 (1997)CrossRefPubMedGoogle Scholar
  9. 9.
    H. Delatte, D.P. Martin, F. Naze, R. Goldbach, B. Reynaud, M. Peterschmitt, J.M. Lett, J. Gen. Virol. 86, 1533–1542 (2005)CrossRefPubMedGoogle Scholar
  10. 10.
    B. Pico, M.J. Diez, F. Nuez, Sci. Hortic. (Amsterdam) 67, 151–196 (1996)CrossRefGoogle Scholar
  11. 11.
    J.E. Polston, P.K. Anderson, Plant. Dis. 81, 1358–1369 (1997)CrossRefGoogle Scholar
  12. 12.
    J.B. Wu, F.M. Dai, X.P. Zhou, Plant. Dis. 90, 1359 (2006)CrossRefGoogle Scholar
  13. 13.
    R.B. Mughra, S.S. Liu, X. Zhou, J. Phytopathol. 156, 217–221 (2008)CrossRefGoogle Scholar
  14. 14.
    Y. Xie, X.P. Zhou, Z.H. Li, Z.K. Zhang, G.X. Li, Chin. Sci. Bull. 47, 1273–1276 (2002)CrossRefGoogle Scholar
  15. 15.
    X.P. Zhou, Y. Xie, Z.K. Zhang, Arch. Virol. 146, 1599–1606 (2001)CrossRefPubMedGoogle Scholar
  16. 16.
    J. Rozas, J.C. Sanchez-DelBarrio, X. Messeguer, R. Rozas, Bioinformatics 19, 2496–2497 (2003)CrossRefPubMedGoogle Scholar
  17. 17.
    F.A. Vanengelen, J.W. Molthoff, A.J. Conner, J.P. Nap, A. Pereira, W.J. Stiekema, Transgenic. Res. 4, 288–290 (1995)CrossRefGoogle Scholar
  18. 18.
    X.P. Zhou, Y. Xie, X.R. Tao, Z.K. Zhang, Z.H. Li, C.M. Fauquet, J. Gen. Virol. 84, 237–247 (2003)CrossRefPubMedGoogle Scholar
  19. 19.
    Y. Xie, T. Jiang, X.P. Zhou, J. Eur. Plant Pathol. 115, 369–375 (2006)CrossRefGoogle Scholar
  20. 20.
    Z.H. Li, X.P. Zhou, X. Zhang, Y. Xie, Arch. Virol. 149, 1721–1732 (2004)PubMedGoogle Scholar
  21. 21.
    Q.Y. Yin, H.Y. Yang, Q.H. Gong, H.Y. Wang, Y.L. Liu, Y.G. Hong, P. Tien, Virus Res. 81, 69–76 (2001)CrossRefPubMedGoogle Scholar
  22. 22.
    Y. Xie, X.P. Zhou, Arch. Virol. 148, 2047–2054 (2003)CrossRefPubMedGoogle Scholar
  23. 23.
    Y.M. Zubiaur, I. Zabalgogeazcoa, C. DeBlas, F. Sanchez, E.L. Peralta, J. Romero, F. Ponz, J. Phytopathol. 144, 277–279 (1996)CrossRefGoogle Scholar
  24. 24.
    J. Navas-Castillo, S. Sanchez-Campos, J.A. Diaz, E. Saez-Alonso, E. Moriones, Plant Dis. 83, 29–32 (1999)CrossRefGoogle Scholar
  25. 25.
    S. Duffy, E.C. Holmes, Appl. Environ. Microbiol. 73, 7114–7117 (2007)CrossRefPubMedGoogle Scholar
  26. 26.
    M. Onuki, Nougyou Engei 75, 108–113 (2000)Google Scholar
  27. 27.
    R.W. Briddon, S.E. Bull, S. Mansoor, I. Amin, P.G. Markham, Mol. Biotechnol. 20, 315–318 (2002)CrossRefPubMedGoogle Scholar
  28. 28.
    J. Jose, R. Usha, Virology 305, 310–317 (2003)CrossRefPubMedGoogle Scholar
  29. 29.
    K. Saunders, I.D. Bedford, R.W. Briddon, P.G. Markham, S.M. Wong, J. Stanley, Proc. Natl Acad. Sci. USA 97, 6890–6895 (2000)CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  1. 1.Key Laboratory of Molecular Biology of Crop Pathogens and Insects of MOA, Institute of BiotechnologyZhejiang UniversityHangzhouPeople’s Republic of China
  2. 2.Horticultural Research InstituteShanghai Academy of Agriculture SciencesShanghaiPeople’s Republic of China

Personalised recommendations