Several tomato production regions in China were surveyed for tomato yellow leaf curl disease (TYLCD), and 31 tomato leaf samples showing TYLCD-like symptoms were collected. The partial or full-length genomes of these isolates were sequenced and tomato yellow leaf curl virus (TYLCV) was detected in Shanghai, Zhejiang, Jiangsu Shandong and Hebei provinces of China. The TYLCV isolates found in China share high sequence identity (>98%) and have more than 97% sequence identity with TYLCV-IL[IL:Reo] (X15656). Phylogenetic relationship analysis reveals that although with little genetic variability, they can form two groups and all the TYLCV isolates in China belong to the group I. An infectious clone of TYLCV-[CN:SH2] (AM282874) was constructed and agro-inoculated into Nicotianabenthamiana, N.tabacum Samsun, N.glutinosa, Solanumlycopersicum, Petuniahybrida, Cucumissativus, Gossypiumhirsutum, S.melongena, and Capsicumannuum. TYLCV-[CN:SH2] can induce severe leaf curling and stunting symptoms in these plants except C.sativus, G.hirsutum, S.melongena and C.annuum. We verified that TYLCV can trans-replicate tomato yellow leaf curl China virus DNA-β in N.benthamiana and S.lycopersicum and induced more severe symptoms with distortion and yellow vein.
Tomato yellow leaf curl virus Sequence Infectivity China
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This work was supported by the National Natural Science Foundation of China (Grants No. 30530520) and the Zhejiang Agricultural Science and Technology Key Research Projects (2007C12054).
C.M. Fauquet, R.W. Briddon, J.K. Brown, E. Moriones, J. Stanley, M. Zerbini, X. Zhou, Arch. Virol. 153, 783–821 (2008)CrossRefPubMedGoogle Scholar