Identification and characterization of a novel Tritimovirus species isolated from wild Trisetum flavescens L., family Poaceae
- 86 Downloads
Yellow oat-grass plants (Trisetum flavescens L.) with mild mosaic and pronounced dwarfing symptoms were observed at different locations in the Czech Republic. Electron microscope observations of symptomatic plants revealed the presence of filamentous particles and inclusion bodies characteristic of the family Potyviridae. The virus was readily mechanically transmitted to its original host plus a narrow host range of monocot species. Serological assays of infected plant extracts using antiserum specific to the closest species in the family Potyviridae were negative. The 3′ end of the viral genome was cloned, sequenced and compared to sequences of species in the family Potyviridae. The virus is more closely related to viruses in the genus Tritimovirus than to other genera within the Potyviridae. Based on phylogenetic analyses of the coat protein cistron and flanking genomic regions, we propose this is a distinct viral species of the genus Tritimovirus, tentatively named Yellow oat-grass mosaic virus (YOgMV).
KeywordsPotyviridae Tritimovirus Virus taxonomy Yellow oat-grass
The authors thank Dr. Keith L. Perry (Cornell University, USA) for the critical reading of the manuscript. We thank Miss. M. Bouzková for her excellent technical assistance. This work was supported by the Ministry of Agriculture of the Czech Republic, Project No. MZe 0002700603.
- 3.P.H. Berger, M.J. Adams, O.W. Barnett, A.A. Brunt, J. Hammond, J.H. Hill, R.L. Jordan, S. Kashiwazaki, E. Rybicki, N. Spence, D.C. Stenger, S.T. Ohki, I. Uyeda, A. van Zaayen, J. Valkonen, H.J. Vetten, in 8th Rep. ICTV, ed. by C.M. Fauquet, M.A. Mayo, J. Maniloff, U. Desselberger, L.A. Ball (Elsevier Academic Press, San Diego, CA, 2005), pp. 819–841Google Scholar
- 4.F. Rabenstein, J. Schubert, Genus Rymovirus, in Virus and Virus Diseases of Poaceae (INRA Publication, Paris, 2004), pp. 395–401Google Scholar
- 8.R. Koenig, D.E. Lesemann, J. Phytopathol. 118, 105–116 (1985)Google Scholar
- 11.J. Sambrook, D.W. Russell, A Laboratory Manual, 3rd edn. (Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, 2001)Google Scholar
- 14.T.A. Hall, Nucleic Acids Symp. Ser. 41, 95–98 (1999)Google Scholar
- 15.K.B. Nicholas, H.B. Nicholas, Pittsburgh Supercomputing Center, Pittsburgh (1997)Google Scholar
- 17.D.D. Shukla, C.W. Ward, A.A. Brunt, CAB International, Wallingford, UK (1994)Google Scholar