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Virus Genes

, Volume 39, Issue 1, pp 146–152 | Cite as

Identification and characterization of a novel Tritimovirus species isolated from wild Trisetum flavescens L., family Poaceae

  • Mohamed Hassan
  • Lenka Širlova
  • Milan Jokeš
  • Josef Vacke
Article

Abstract

Yellow oat-grass plants (Trisetum flavescens L.) with mild mosaic and pronounced dwarfing symptoms were observed at different locations in the Czech Republic. Electron microscope observations of symptomatic plants revealed the presence of filamentous particles and inclusion bodies characteristic of the family Potyviridae. The virus was readily mechanically transmitted to its original host plus a narrow host range of monocot species. Serological assays of infected plant extracts using antiserum specific to the closest species in the family Potyviridae were negative. The 3′ end of the viral genome was cloned, sequenced and compared to sequences of species in the family Potyviridae. The virus is more closely related to viruses in the genus Tritimovirus than to other genera within the Potyviridae. Based on phylogenetic analyses of the coat protein cistron and flanking genomic regions, we propose this is a distinct viral species of the genus Tritimovirus, tentatively named Yellow oat-grass mosaic virus (YOgMV).

Keywords

Potyviridae Tritimovirus Virus taxonomy Yellow oat-grass 

Notes

Acknowledgments

The authors thank Dr. Keith L. Perry (Cornell University, USA) for the critical reading of the manuscript. We thank Miss. M. Bouzková for her excellent technical assistance. This work was supported by the Ministry of Agriculture of the Czech Republic, Project No. MZe 0002700603.

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Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Mohamed Hassan
    • 1
    • 2
  • Lenka Širlova
    • 2
  • Milan Jokeš
    • 2
  • Josef Vacke
    • 2
  1. 1.Agricultural Botany Department (Plant Pathology Sector), Faculty of AgricultureFayoum UniversityFayoumEgypt
  2. 2.Department of VirologyCrop Research InstitutePrague 6Czech Republic

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