Virus Genes

, 39:90

Detection of a new bat gammaherpesvirus in the Philippines

  • Shumpei Watanabe
  • Naoya Ueda
  • Koichiro Iha
  • Joseph S. Masangkay
  • Hikaru Fujii
  • Phillip Alviola
  • Tetsuya Mizutani
  • Ken Maeda
  • Daisuke Yamane
  • Azab Walid
  • Kentaro Kato
  • Shigeru Kyuwa
  • Yukinobu Tohya
  • Yasuhiro Yoshikawa
  • Hiroomi Akashi
Article

DOI: 10.1007/s11262-009-0368-8

Cite this article as:
Watanabe, S., Ueda, N., Iha, K. et al. Virus Genes (2009) 39: 90. doi:10.1007/s11262-009-0368-8

Abstract

A new bat herpesvirus was detected in the spleen of an insectivorous bat (Hipposideros diadema, family Hipposideridae) collected on Panay Island, the Philippines. PCR analyses were performed using COnsensus-DEgenerate Hybrid Oligonucleotide Primers (CODEHOPs) targeting the herpesvirus DNA polymerase (DPOL) gene. Although we obtained PCR products with CODEHOPs, direct sequencing using the primers was not possible because of high degree of degeneracy. Direct sequencing technology developed in our rapid determination system of viral RNA sequences (RDV) was applied in this study, and a partial DPOL nucleotide sequence was determined. In addition, a partial gB gene nucleotide sequence was also determined using the same strategy. We connected the partial gB and DPOL sequences with long-distance PCR, and a 3741-bp nucleotide fragment, including the 3′ part of the gB gene and the 5′ part of the DPOL gene, was finally determined. Phylogenetic analysis showed that the sequence was novel and most similar to those of the subfamily Gammaherpesvirinae.

Keywords

Bat virus Direct sequencing Herpesvirus Virus discovery RDV 

Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Shumpei Watanabe
    • 1
  • Naoya Ueda
    • 2
  • Koichiro Iha
    • 2
  • Joseph S. Masangkay
    • 3
  • Hikaru Fujii
    • 1
  • Phillip Alviola
    • 4
  • Tetsuya Mizutani
    • 5
  • Ken Maeda
    • 6
  • Daisuke Yamane
    • 1
  • Azab Walid
    • 1
  • Kentaro Kato
    • 1
  • Shigeru Kyuwa
    • 2
  • Yukinobu Tohya
    • 1
  • Yasuhiro Yoshikawa
    • 2
  • Hiroomi Akashi
    • 1
  1. 1.Department of Veterinary Microbiology, Graduate School of Agricultural and Life SciencesUniversity of TokyoTokyoJapan
  2. 2.Department of Biomedical Science, Graduate School of Agricultural and Life SciencesUniversity of TokyoTokyoJapan
  3. 3.College of Veterinary MedicineUniversity of the Philippines Los Baños, CollegeLagunaPhilippines
  4. 4.Museum of Natural HistoryUniversity of the Philippines Los Baños, CollegeLagunaPhilippines
  5. 5.Department of Virology 1National Institute of Infectious DiseasesTokyoJapan
  6. 6.Laboratory of Veterinary Microbiology, Faculty of AgricultureYamaguchi UniversityYamaguchiJapan

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