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Genome organization and phylogenetic relationship of Pineapple mealybug wilt associated virus-3 with family Closteroviridae members

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The nucleotide sequence of Pineapple mealybug wilt associated virus-3 (PMWaV-3) (Closteroviridae: Ampelovirus), spanning seven open reading frames (ORFs) and the untranslatable region of the 3′ end was determined. Based on the amino acid identities with orthologous ORFs of PMWaV-1 (54%–73%) and PMWaV-2 (13%–35%), we propose PMWaV-3 is a new species in the PMWaV complex. PMWaV-3 lacks an intergenic region between ORF1b and ORF2, encodes a relatively small, 28.8 kDa, coat protein, and lacks a coat protein duplicate. Phylogenetic analyses were used to analyze seven different domains and ORFs from members of the family Closteroviridae. Two distinct clades within the recognized genus Ampelovirus were observed; one that includes PMWaV-3 and PMWaV-1 and several GLRaVs and another that includes PMWaV-2 and GLRaV-3, the type member of the genus Ampelovirus.

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Fig. 1


  1. 1.

    E.G. Borroto, M. Cintra, J. Gonzalez, C. Borroto, P. Oramas. Plant Dis. 82, 263 (1998). doi:

  2. 2.

    C.F. Gambley, V. Steele, A.D.W. Geering, J.E. Thomas, Australas. Plant Pathol. 37, 95–105 (2008). doi:

  3. 3.

    J.S. Hu, A. Gonsalves, D. Sether, D.E. Ullman, Acta Hortic 334, 411–416 (1993)

  4. 4.

    J.S. Hu, D.M. Sether, D.E. Ullman, Plant Pathol. 45, 829–836 (1996). doi:

  5. 5.

    J.S. Hu, D.M. Sether, X.P. Liu, M. Wang, F. Zee, D. Ullman, Plant Dis. 81, 1150–1154 (1997). doi:

  6. 6.

    D.M. Sether, A.V. Karasev, C. Okumura, C. Arakawa, F. Zee, M.M. Kislan, J.L. Busto, J.S. Hu, Plant Dis. 85, 856–864 (2001). doi:

  7. 7.

    D.M. Sether, M.J. Melzer, J.L. Busto, F. Zee, J.S. Hu, Plant Dis. 89, 450–456 (2005). doi:

  8. 8.

    W. Wakman, D. Teakle, J.E. Thomas, R.G. Dietzgen, Aust. J. Agric. Sci. 46, 947–958 (1995). doi:

  9. 9.

    D.M. Sether, J.S. Hu, D.E. Ullman, Phytopathology 88, 1224–1230 (1998). doi:

  10. 10.

    D.M. Sether, J.S. Hu, Phytopathology 92, 928–935 (2002). doi:

  11. 11.

    D.M. Sether, J.S. Hu, Plant Dis. 86, 867–874 (2002). doi:

  12. 12.

    M.J. Melzer, D.M. Sether, A.V. Karasev, J.S. Hu, Arch. Virol. 153, 707–714 (2008). doi:

  13. 13.

    M.J. Melzer, A.V. Karasev, D.M. Sether, J.S. Hu, J. Gen. Virol. 82, 1–7 (2001)

  14. 14.

    S.F. Atschul, W. Gish, W. Miller, E.W. Myers, J.C. Wooten, Matur. Gen. 6, 119–129 (1994)

  15. 15.

    S.F. Altschul, T.L. Madden, A.A. Schäffer, J. Zhang, Z. Zhang, W. Miller, D.J. Lipman, Nucleic Acids Res. 25, 3389–3402 (1997). doi:

  16. 16.

    J.D. Thompson, T.J. Gibson, F. Plewniak, F. Jeanmougin, D.G. Higgins, Nucleic Acids Res. 24, 4876–4882 (1997). doi:

  17. 17.

    D.L. Swofford, PAUP*, PAUP*: Phylogenetics Using Parsimony (*and Other Methods) (Sinauer Associates, Inc., Sunderland, MA, 2000)

  18. 18.

    K.-S. Ling, H.-Y. Zhu, R.F. Drong, J.L. Slightom, J.R. McFerson, D. Gonsalves, J. Gen. Virol. 79, 1299–1307 (1998)

  19. 19.

    A. Marchler-Bauer, J.B. Anderson, M.K. Derbyshire, C. De Weese-Scott, N.R. Gonzales, M. Gwadz, L. Hao, S. He, D.I. Hurwitz, J.D. Jackson, Z. Ke, D. Krylov, C.J. Lanczycki, C.A. Liebert, C. Liu, F. Lu, S. Lu, G.H. Marchler, M. Mullokandov, J.S. Song, N. Thanki, R.A. Yamashita, J.J. Yin, D. Zhang, S.H. Bryant, Nucleic Acids Res. 35, D237–D240 (2007). doi:

  20. 20.

    A.A. Agranovsky, E.V. Koonin, V.P. Boyko, E. Maiss, R. Frötschl, N.A. Lunina, J.G. Atabekov, Virology 198, 311–324 (1994). doi:

  21. 21.

    M. Alrwahnih, J.K. Uyemoto, B.W. Falk, A. Rowhani, Arch. Virol. 152, 2197–2206 (2007). doi:

  22. 22.

    V.V. Dolja, J.F. Kreuze, J.P.T. Valkonen, Virus Res. 117, 38–51 (2006). doi:

  23. 23.

    T. Satyanarayana, S. Gowda, M. Mawassi, M.R. Albiach-Marti, M.A. Ayllón, C. Robertson, S.M. Garnsey, W.O. Dawson, Virology 278, 253–265 (2000). doi:

  24. 24.

    L.Y. Geer, M. Domrachev, D.J. Lipman, S.H. Bryant, Genome Res. 12, 1619–1623 (2002). doi:

  25. 25.

    E.V. Koonin, A.R. Mushegian, E.V. Ryabov, V.V. Dolja, J. Gen. Virol. 72, 2895–2903 (1991). doi:

  26. 26.

    H.R. Pappu, A.V. Karasev, E.J. Anderson, S.S. Pappu, M.E. Hilf, V.J. Febres, R.M.G. Eckloff, M. McCaffery, V. Byoko, S. Gowda, V.V. Dolja, E.V. Koonin, D.J. Gumpf, K.C. Cline, S.M. Garnsey, W.O. Dawson, R.F. Lee, C.L. Niblett, Virology 199, 35–46 (1994). doi:

  27. 27.

    H.Y. Zhu, K.S. Ling, D.E. Goszczynski, J.R. McFerson, D. Gonsalves, J. Gen. Virol. 79, 1289–1298 (1998)

  28. 28.

    M. Beuve, L. Sempe, O. Lemaire, J. Virol. Methods 141, 117–124 (2007). doi:

  29. 29.

    V.I. Maliogka, C.I. Dovas, N.I. Katis, Virus Res. 135, 125–135 (2008). doi:

  30. 30.

    H.J. Maree, M.J. Freeborough, J.T. Burger, Arch. Virol. 153, 755–757 (2008). doi:

  31. 31.

    V.V. Dolja, V.P. Boyko, A.A. Agranovsky, E.V. Koonin, Virology 184, 79–86 (1991). doi:

  32. 32.

    C. Büchen-Osmond (ed.), Index of Viruses—Closteroviridae. (ICTVdB—The Universal Virus Database, version 4., Columbia University, New York, USA, 2006). Accessed 01 August 2008

  33. 33.

    B.G. Hall, Phylogenetic Trees Made Easy. A How-To Manual, 2nd edn. (Sinauer Associates, Inc., Sunderland, MA, 2004), p. 221

  34. 34.

    D.M. Sether, J.S. Hu, Australas. Plant Pathol. 30, 31–36 (2001). doi:

  35. 35.

    B.S. Sipes, D.M. Sether, J.S. Hu, Plant Dis. 86, 933–938 (2002). doi:

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This research funded, in part, by a Grant from the Hawaii Department of Agriculture (Contract No. 49048), a Grant through the Tropical/Subtropical Agricultural Research Program (Project No. HAW00985-10145), a grant from the United States Department of Agriculture-Cooperative State Research, Education, and Extension Service (USDA-CSREES) Grants Program-Pest Management Alternatives Program (Project No. HAW00937-G under agreement 2003-34381-13659), a Grant from the USDA-CSREES Regional Integrated Pest Management Competitive Grants Program-Western Region (Project No. Haw01951-G under agreement 2006-34103-17176).

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Correspondence to John S. Hu.

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Sether, D.M., Melzer, M.J., Borth, W.B. et al. Genome organization and phylogenetic relationship of Pineapple mealybug wilt associated virus-3 with family Closteroviridae members. Virus Genes 38, 414–420 (2009).

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  • Virus genome
  • Pineapple
  • Mealybug wilt
  • Ananas comosus
  • Virus transmission