Genetic characterization of two low pathogenic avian influenza virus H5N1 isolates from Ontario, Canada
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Genomes of two low pathogenic H5N1 avian influenza (LPAI) viruses, A/Turkey/ON/84/1983 and A/Mallard/ON/499/2005 from Ontario, Canada were cloned and genetically characterized. Phylogenetic analysis showed that the Canadian isolates cluster with other North American AIVs and are distinct from the Euro-Asian H5N1 isolates. Individual gene comparisons demonstrated that the Ontario isolates were most similar to the viruses isolated from around the same time period and geographical area. A long deletion of 22 amino acids was identified in the stalk region of NA of A/Turkey/ON/84/1983 isolate, a characteristic mutation related to its adaptation to domestic birds. To our knowledge A/Turkey/ON/84/1983 genomic sequence is the first and only available entire genomic sequence of a H5N1 AIV from domestic birds in Canada and USA.
KeywordsAvian influenza virus H5N1 Canada Genetic characterization
This study was supported by Poultry Industry Council, Canadian Poultry Research Council, Saskatchewan Chicken Industry Development Fund, the Canadian Cooperative Wildlife Health Centre, Ontario Ministry of Health, and the NSERC-AAFC Partnerships program.
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