Virus Genes

, Volume 37, Issue 3, pp 392–399 | Cite as

Comparative sequence analyses of a new mammalian reovirus genome and the mammalian reovirus S1 genes from six new serotype 2 human isolates

  • Lihua Song
  • Yusen Zhou
  • Jun He
  • Hong Zhu
  • Rutong Huang
  • Panyong Mao
  • Qing Duan
Article

Abstract

We previously described isolation of a potentially new mammalian reovirus, designated BYD1, which can cause clinical symptoms similar to that of severe acute respiratory syndrome (SARS) in guinea pigs and macaques, from throat swabs of one SARS patient of Beijing, in 2003. For this study, we determined the genome sequences of BYD1 and the S1 gene sequences of other five mammalian reovirus isolates (BLD, JP, and BYL were isolated from different SARS patients during the outbreak, 302I and 302II were isolated from fecal specimens of two children of Beijing in 1982) to allow molecular comparison with other previously reported mammalian reoviruses (MRVs). Comparative analyses of the BYD1 genome with those of three prototype mammalian reovirus strains demonstrated that BYD1 is a novel reassortant virus, with its S1 gene segment coming from a previously unidentified serotype 2 isolate and other nine segments coming from ancestors of homologous T1L and T3D segments, which supports the hypothesis that mammalian reovirus gene segments reassort in nature. Further analyses of the S1 segments of the six isolates showed that all the isolates are novel serotype 2 MRVs based on their S1 gene sequences, which are markedly different from those of all previously reported, and the S1 genes of the four new isolates share more than 99% identity with each other, proving that they diverged from a common ancestor most recently, and the S1 genes of the four new isolates share about 65% identity with those of the two strains isolated in 1982.

Keywords

dsRNA Virus Mammalian reovirus Orthoreovirus Virus genome 

Notes

Acknowledgments

This study was supported by a National Natural Science Foundation of China (No. 30471555) and the Initiative Foundation for Scientific and Technological Innovation of Academic Military Medical Sciences (No. 200408183).

References

  1. 1.
    M.L. Nibert, L.A. Schiff, in Fields Virology, 4th edn, ed. by D.M. Knipe, P.M. Howley (Lippincot Williams & Wilkins Inc., Philadelphia, 2001), p. 1679Google Scholar
  2. 2.
    P. Roy, in Fields Virology, 4th edn, ed. by D.M. Knipe, P.M. Howley (Lippincot Williams & Wilkins Inc., Philadelphia, 2001), p. 1835Google Scholar
  3. 3.
    M.K. Estes, in Fields Virology, 4th edn, ed. by D.M. Knipe, P.M. Howley (Lippincot Williams & Wilkins Inc., Philadelphia, 2001), p. 1747Google Scholar
  4. 4.
    K.L. Tyler, in Fields Virology, 4th edn, ed. by D.M. Knipe, P.M. Howley (Lippincot Williams & Wilkins Inc., Philadelphia, 2001), p. 1725Google Scholar
  5. 5.
    A.J. Shatkin, J.D. Sipe, P. Loh, J. Virol. 2, 986–991 (1968)PubMedPubMedCentralGoogle Scholar
  6. 6.
    J.R. Wiener, W.K. Joklik, Virology 169, 194–203 (1989)PubMedCrossRefGoogle Scholar
  7. 7.
    L.A. Breun, T.J. Broering, A.M. McCutcheon, S.J. Harrison, C.L. Luongo, M.L. Nibert, Virology 287, 333–348 (2001)PubMedCrossRefGoogle Scholar
  8. 8.
    P. Yin, N.D. Keirstead, T.J. Broering, M.M. Arnold, J.S. Parker, M.L. Nibert, K.M. Coombs, Virol. J. 1, 6 (2004)PubMedPubMedCentralCrossRefGoogle Scholar
  9. 9.
    J. Jiang, L. Hermann, K.M. Coombs, Virus Genes 33, 193–204 (2006)PubMedCrossRefGoogle Scholar
  10. 10.
    E. Fajardo, A.J. Shatkin, Virology 178, 223–231 (1990)PubMedCrossRefGoogle Scholar
  11. 11.
    B.A. Belli, C.E. Samuel, Virology 193, 16–27 (1993)PubMedCrossRefGoogle Scholar
  12. 12.
    A.B. Sabin, Science 130, 1387–1389 (1959)PubMedCrossRefGoogle Scholar
  13. 13.
    K.L. Tyler, E.S. Barton, M.L. Ibach, C. Robinson, J.A. Campbell, S.M. O’Donnell, T. Valyi-Nagy, P. Clarke, J.D. Wetzel, T.S. Dermody, J. Infect. Dis. 189, 1664–1675 (2004)PubMedCrossRefGoogle Scholar
  14. 14.
    P.J. Johansson, T. Sveger, K. Ahlfors, J. Ekstrand, L. Svensson, Scand. J. Infect. Dis. 28, 117–120 (1996)PubMedCrossRefGoogle Scholar
  15. 15.
    L. Hermann, J. Embree, P. Hazelton, B. Wells, R.T. Coombs, Pediatr. Infect. Dis. J. 23, 373–375 (2004)PubMedCrossRefGoogle Scholar
  16. 16.
    K.B. Chua, G. Crameri, A. Hyatt, M. Yu, M.R. Tompang, J. Rosli, J. McEachern, S. Crameri, V. Kumarasamy, B.T. Eaton, L.F. Wang, Proc. Natl. Acad. Sci. U.S.A. 104, 11424–11429 (2007)PubMedPubMedCentralCrossRefGoogle Scholar
  17. 17.
    Q. Duan, H. Zhu, Y. Yang, W. Li, Y. Zhou, J. He, K. He, H. Zhang, T. Zhou, L. Song, Y. Gan, H. Tan, B. Jin, H. Li, T. Zuo, D. Chen, X. Zhang, Chin. Sci. Bull. 48, 1369–1372 (2003)CrossRefGoogle Scholar
  18. 18.
    T. Zuo, H. Tan, J. He, H. Zhu, H. Zhang, L. Song, R. Huang, Q. Duan, Bull. Acad. Mil. Med. Sci. 27, 241–243 (2003)Google Scholar
  19. 19.
    C. He, W. Pang, X. Yong, H. Zhu, M. Lei, Q. Duan, DNA Cell Biol. 24, 491–495 (2005)PubMedCrossRefGoogle Scholar
  20. 20.
    L. Liang, C. He, M. Lei, S. Li, Y. Hao, H. Zhu, Q. Duan, DNA Cell Biol. 24, 485–490 (2005)PubMedCrossRefGoogle Scholar
  21. 21.
    Y. Su, J. He, H. Zhu, L. Song, R. Huang, Q. Duan, Bull. Acad. Mil. Med. Sci. 29, 418–420 (2005)Google Scholar
  22. 22.
    S. Wu, Y. Li, W. Fan, S. Wu, X. Wei, S. Tong, Z. Wang, R. Zhang, Y. Li, Zhonghua Yi Xue Za Zhi 62, 146–151 (1982)PubMedGoogle Scholar
  23. 23.
    J.R. Diaz-Ruiz, J.M. Kaper, Prep. Biochem. 8, 1–17 (1978)PubMedGoogle Scholar
  24. 24.
    P.R. Lambden, S.J. Cooke, E.O. Caul, I.N. Clarke, J. Virol. 66, 1817–1822 (1992)PubMedPubMedCentralGoogle Scholar
  25. 25.
    J.D. Thompson, T.J. Gibson, F. Plewniak, F. Jeanmougin, D.G. Higgins, Nucleic Acids Res. 25, 4876–4882 (1997)PubMedPubMedCentralCrossRefGoogle Scholar
  26. 26.
    M. Nei, T. Gojobori, Mol. Biol. Evol. 3, 418–426 (1986)PubMedGoogle Scholar
  27. 27.
    S. Kumar, K. Tamura, M. Nei, Brief Bioinform. 5, 150–163 (2004)PubMedCrossRefGoogle Scholar
  28. 28.
    C. Combet, C. Blanchet, C. Geourjon, G. Deleage, Trends Biochem. Sci. 25, 147–150 (2000)PubMedCrossRefGoogle Scholar
  29. 29.
    J.A. Campbell, P. Schelling, J.D. Wetzel, E.M. Johnson, J.C. Forrest, G.A. Wilson, M. Aurrand-Lions, B.A. Imhof, T. Stehle, T.S. Dermody, J. Virol. 79, 7967–7978 (2005)PubMedPubMedCentralCrossRefGoogle Scholar
  30. 30.
    W.K. Joklik, M.R. Roner, Prog. Nucleic Acid Res. Mol. Biol. 53, 249–281 (1996)PubMedCrossRefGoogle Scholar
  31. 31.
    M.M. Becker, T.R. Peters, T.S. Dermody, J. Virol. 77, 5948–5963 (2003)PubMedPubMedCentralCrossRefGoogle Scholar
  32. 32.
    Y. Sun, S.J. Xin, H.H. Shen, Y. Hu, D.P. Xu, H. Zhu, L. Zhu, Q. Duan, P.Y. Mao, Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi 20, 66–68 (2006)PubMedGoogle Scholar
  33. 33.
    R. Walgate, Scientist (2003), http://www.biomedcentral.com/news/20030520/03/
  34. 34.
    M. Fan, Z. Li, Chin. J. Epidemiol. 25, 1078–1080 (2004)Google Scholar

Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Lihua Song
    • 1
  • Yusen Zhou
    • 1
  • Jun He
    • 1
  • Hong Zhu
    • 1
  • Rutong Huang
    • 1
  • Panyong Mao
    • 2
  • Qing Duan
    • 1
  1. 1.State Key Laboratory of Pathogen and BiosecurityInstitute of Microbiology and EpidemiologyBeijingChina
  2. 2.Department of VirologyBeijingChina

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