Virus Genes

, 37:250

Genomic characterization of a novel group A lamb rotavirus isolated in Zaragoza, Spain

  • Max Ciarlet
  • Christian Hoffmann
  • Eleonora Lorusso
  • Rafael Baselga
  • Maria Assunta Cafiero
  • Krisztián Bányai
  • Jelle Matthijnssens
  • Viviana Parreño
  • Simona de Grazia
  • Canio Buonavoglia
  • Vito Martella
Article

Abstract

An ovine rotavirus (OVR) strain, 762, was isolated from a 30-day-old lamb affected with severe gastroenteritis, in Zaragoza, Spain, and the VP4, VP7, VP6, NSP4, and NSP5/NSP6 genes were subsequently characterized molecularly. Strain OVR762 was classified as a P[14] rotavirus, as the VP4 and VP8* trypsin-cleavage product of the VP4 protein revealed the highest amino acid (aa) identity (94% and 97%, respectively) with that of the P11[14] human rotavirus (HRV) strain PA169, isolated in Italy. Analysis of the VP7 gene product revealed that OVR762 possessed G8 serotype specificity, a type common in ruminants, with the highest degree of aa identity (95–98%) shared with serotype G8 HRV, bovine rotavirus, and guanaco (Lama guanicoe) rotavirus strains. Moreover, strain OVR762 displayed a bovine-like NSP4 (genotype E2) and NSP5/NSP6 (genotype H3), and a VP6 genotype I2, as well as a long electropherotype pattern. This is the first report of a lamb rotavirus with P[14] and G8 specificities, providing additional evidence for the wide genetic and antigenic diversity of group A rotaviruses.

Keywords

Rotavirus Gastroenteritis Genome Genotype Genetic diversity Reassortment Lamb VP4 VP6 VP7 NSP4 NSP5 

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Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Max Ciarlet
    • 1
  • Christian Hoffmann
    • 2
  • Eleonora Lorusso
    • 3
  • Rafael Baselga
    • 4
  • Maria Assunta Cafiero
    • 5
  • Krisztián Bányai
    • 6
  • Jelle Matthijnssens
    • 7
  • Viviana Parreño
    • 8
  • Simona de Grazia
    • 9
  • Canio Buonavoglia
    • 3
  • Vito Martella
    • 3
  1. 1.Vaccine & Biologics – Clinical ResearchMerck Research LaboratoriesNorth WalesUSA
  2. 2.Department of MicrobiologyUniversity of PennsylvaniaPhiladelphiaUSA
  3. 3.Dipartimento di Sanità e Benessere AnimaleFacoltà di Medicina Veterinaria di BariBariItaly
  4. 4.ExopolZaragozaSpain
  5. 5.Istituto Zooprofilattico di Puglia e BasilicataSezione di FoggiaItaly
  6. 6.Veterinary Medical Research InstituteHungarian Academy of SciencesBudapestHungary
  7. 7.Department of Microbiology & Immunology, Rega Institute for Medical ResearchUniversity of LeuvenLeuvenBelgium
  8. 8.Virology Institute, CICV y ANational Institute of Agriculture Technology (INTA)Buenos AiresArgentina
  9. 9.Dipartimento di Igiene e MicrobiologiaUniversità di PalermoPalermoItaly

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