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Virus Genes

, Volume 36, Issue 2, pp 393–400 | Cite as

Construction and immune response characterization of a recombinant pseudorabies virus co-expressing capsid precursor protein (P1) and a multiepitope peptide of foot-and-mouth disease virus in swine

  • Yannan He
  • Ping Qian
  • Keshan Zhang
  • Qingxia Yao
  • Dang Wang
  • Zhuofei Xu
  • Bin Wu
  • Meilin Jin
  • Shaobo XiaoEmail author
  • Huanchun ChenEmail author
Article

Abstract

Foot-and-mouth disease (FMD) is the most contagious and devastating disease of livestock. Our previous studies demonstrated that TK/gG/P1+, a recombinant expressing the FMDV capsid precursor protein (P1) based on attenuated pseudorabies virus (PRV) TK/gG, could be used as a recombinant vaccine to protect pigs against both pseudorabies and FMD. However, because the P1 expression cassette is inserted into the gG locus of the genome of PRV TK/gG, this bivalent vaccine cannot be used in conjunction with the PRV gE-ELISA, an extensively used discriminatory serological test in eradication programs for pseudorabies, which limits the clinical use of this bivalent vaccine. To circumvent this shortcoming, in this study, an expression cassette containing synthetic multiepitope gene “FHG” consisting of six potential B cell epitopes and two potential T cell epitopes of FMDV, under the control of CMV promoter, was further inserted into the gE/gI locus of genome of TK/gG/P1+, resulting in a new recombinant FHG/P1/PRV. The immunogenicity of FHG/P1/PRV was evaluated and compared with TK/gG/P1+ in piglets. Our results clearly showed that FHG/P1/PRV performed better than or comparable with TK-/gG-/P1+, as demonstrated by comparable PRV-specific neutralizing antibodies, enhanced FMDV-specific neutralizing antibodies, and cellular immune responses. More importantly, no gE- and gG-specific antibodies could be detected in pigs immunized with FHG/P1/PRV. These data indicate that FHG/P1/PRV is a promising bivalent vaccine candidate with more extensive potential application than TK/gG/P1+ against both pseudorabies and FMD.

Keywords

Foot-and-mouth disease virus Pseudorabies virus Bivalent vaccine Multiepitope gE/gG-ELISA Immune response 

Notes

Acknowledgments

This work was supported by the National Basic Research Program (973) of China (2005CB523200), State 863 High Technology R&D Project of China (2006AA10A204), and the National Natural Sciences Foundation of China (No. 30400322).

References

  1. 1.
    N.J. Knowles, A.R. Samuel, Virus Res. 91, 65–80 (2003)CrossRefGoogle Scholar
  2. 2.
    M.J. Grubman, B. Baxt, Clin. Microbiol. Rev. 17, 465–493 (2004)CrossRefGoogle Scholar
  3. 3.
    P.W. Mason, M.J. Grubman, B. Baxt, Virus Res. 91, 9–32 (2003)CrossRefGoogle Scholar
  4. 4.
    T.R. Doel, Virus Res. 91, 81–89 (2003)CrossRefGoogle Scholar
  5. 5.
    M.J. Grubman, Biologicals 33, 227–234 (2005)CrossRefGoogle Scholar
  6. 6.
    X.J. Shi, B. Wang, M. Wang, Vaccine 25, 1257–1264 (2007)CrossRefGoogle Scholar
  7. 7.
    C. Carrillo, A. Wigdorovitz, J.C. Oliveros, P.I. Zamorano, A.M. Sadir, N. Gomez, J. Salinas, J.M. Escribino, M.V. Borca, J. Virol. 72, 1688–1690 (1998)PubMedPubMedCentralGoogle Scholar
  8. 8.
    C.Y. Wang, T.Y. Chang, A.M. Walfield, J. Ye, M. Shen, S.P. Chen, M.C. Li, Y.L. Lin, M.H. Jong, P.C. Yang, N. Chyr, E. Kramer, F. Brown, Vaccine 20, 2603–2610 (2002)CrossRefGoogle Scholar
  9. 9.
    S.A. Kim, C.M. Liang, I.C. Cheng, Y.C. Cheng, M.T. Chiao, C.J. Tseng, F. Lee, M.H. Jong, M.H. Tao, N.S. Yang, S.M. Liang, J. Gene Med. 8, 1182–1191 (2006)CrossRefGoogle Scholar
  10. 10.
    H. Jin, Y. Kang, C. Xiao, K. Zhu, Y. Ma, Q. Xie, C. He, Z. Yang, Z. Sun, X. Zhang, M. Chen, F. Zhang, B. Wang, Viral Immunol. 18, 539–548 (2005)CrossRefGoogle Scholar
  11. 11.
    Y. Li, N. Aggarwal, H.H. Takamatsu, C.M. Sterling, C. Voyce, P.V. Barnett, Vaccine 24, 4602–4606 (2006)CrossRefGoogle Scholar
  12. 12.
    F. Wang, X.W. He, L. Jiang, D. Ren, Y. He, D.A. Li, S.H. Sun, Vaccine 24, 2017–2026 (2006)CrossRefGoogle Scholar
  13. 13.
    M.P. Moraes, G.A. Mayr, P.W. Mason, M.J. Grubman, Vaccine 20, 1631–1639 (2002)CrossRefGoogle Scholar
  14. 14.
    M. Zheng, N. Jin, H. Zhang, M. Jin, H. Lu, M. Ma, C. Li, G. Yin, R. Wang, Q. Liu, J. Virol. Methods 136, 230–237 (2006)CrossRefGoogle Scholar
  15. 15.
    P. Qian, X. Li, M. Jin, G. Peng, H. Chen, Vaccine 22, 2129–2136 (2004)CrossRefGoogle Scholar
  16. 16.
    M.L. Jin, X.M. Li, P. Qian, D.C. Guo, Z.F. Xu, H.C. Chen, Chin. J. Vet. Sci. 24, 525–527 (2004)Google Scholar
  17. 17.
    T.C. Mettenleiter, Vet. Res. 31, 99–115 (2000)PubMedGoogle Scholar
  18. 18.
    H.C. Chen, L.R. Fang, Q.G. He, M.L. Jin, X.F. Suo, M.Z. Wu, Acta Vet. Zootechnica Sin. 29, 156–161 (1998)Google Scholar
  19. 19.
    Y.B. Jiang, L.R. Fang, S.B. Xiao, H. Zhang, Y.F. Pan, R. Luo, B. Li, H.C. Chen, Vaccine 25, 547–560 (2007)CrossRefGoogle Scholar
  20. 20.
    X. Yu, S. Xiao, L. Fang, Y. Jiang, H. Chen, Virus Genes 33, 337–344 (2006)CrossRefGoogle Scholar
  21. 21.
    E. Blanco, G.-M. Briones, A. Sanz-Parra, P. Gomes, E. De Oliveira, M.L. Valero, D. Andreu, V. Ley, F. Sobrino, J. Virol. 75, 3164–3174 (2001)CrossRefGoogle Scholar
  22. 22.
    H.Y. Zhang, S.H. Sun, Y.J. Guo, F.J. Zhou, Z.H. Chen, Y. Lin, K. Shi, Vaccine 21, 4704–4707 (2003)CrossRefGoogle Scholar
  23. 23.
    A.P. Souza, L. Haut, A. Reyes-Sandoval, A.R. Pinto, Braz. J. Med. Biol. Res. 38, 509–522 (2005)CrossRefGoogle Scholar
  24. 24.
    B.J. Hooft van Iddekinge, N. de Wind, G. Wensvoort, T.G. Kimman, A.L. Giekens, R.J. Moormann, Vaccine 14, 6–12 (1996)CrossRefGoogle Scholar
  25. 25.
    M. van Zijl, G. Wensvoort, E. de Kluyver, M. Hulst, H. van der Gulden, A. Gielkens, A. Berns, R. Moormann, J. Virol. 65, 2761–2765 (1991)PubMedGoogle Scholar
  26. 26.
    H.J. Qiu, Z.J. Tian, G.Z. Tong, Y.J. Zhou, J.Q. Ni, Y.Z. Luo, Vet. Immunol. Immunopathol. 106, 309–319 (2005)CrossRefGoogle Scholar
  27. 27.
    Y. Song, M. Jin, S. Zhang, X. Xu, S. Xiao, S. Cao, H. Chen, Vet. Microbiol. 119, 97–104 (2007)CrossRefGoogle Scholar
  28. 28.
    H.T. Wong, S.C. Cheng, E.W. Chan, Z.T. Sheng, W.Y. Yan, Z.X. Zheng, Y. Xie, Virology 278, 27–35 (2000)CrossRefGoogle Scholar
  29. 29.
    G. Li, W. Chen, W. Yan, K. Zhao, M. Liu, J. Zhang, L. Fei, Q. Xu, Z. Sheng, Y. Lu, Z. Zheng, Virology 328, 274–281 (2004)CrossRefGoogle Scholar
  30. 30.
    H.T. Wong, S.C. Cheng, E.W. Chan, Z.T. Sheng, W.Y. Yan, Z.X. Zheng, Y. Xie, Virology 278, 27–35 (2000)CrossRefGoogle Scholar
  31. 31.
    L. Cedillo-Barron, M. Foster-Cuevas, A. Cook, B. Gutierrez-Castaxeda, S. Kollnberger, F. Leferre, R.M. Parkhouse, Vaccine 21, 4261–4269 (2003)CrossRefGoogle Scholar
  32. 32.
    B.J. Hohlich, K.H. Wiesmuller, B. Haas, W. Gerner, R. Correa, H.R. Hehnen, T. Schlapp, E. Pfaff, A. Saalmuller, J. Gen. Virol. 84, 3315–3324 (2003)CrossRefGoogle Scholar
  33. 33.
    L. Jiang, Q. Li, M. Li, Z. Zhou, L. Wu, J. Fan, Q. Zhang, H. Zhu, Z. Xu, Vaccine 24, 109–115 (2006)CrossRefGoogle Scholar
  34. 34.
    B. Borrego, P. Fernandez-Pacheco, L. Ganges, N. Domenech, N. Fernandez-Borges, F. Sobrino, F. Rodriguez, Vaccine 24, 3889–3899 (2006)CrossRefGoogle Scholar
  35. 35.
    T.S. McKenna, E. Rieder, J. Lubroth, T. Burrage, B. Baxt, P.W. Mason, J. Biotechnol. 44, 83–89 (1996)CrossRefGoogle Scholar
  36. 36.
    A. Sanz-Parra, M.A. Jimenez-Clavero, M.M. Garcia-Briones, E. Blanco, F. Sobrino, V. Ley, Virology 259, 129–134 (1999)CrossRefGoogle Scholar
  37. 37.
    A. Sanz-Parra, B. Vazquez, F. Sobrino, S.J. Cox, V. Ley, J.S. Salt, J. Gen. Virol. 80, 671–679 (1999)CrossRefGoogle Scholar
  38. 38.
    M.M. Garcia-Briones, E. Blanco, C. Chiva, U. Andre, V. Ley, F. Sobrino, Virology 322, 264–275 (2004)CrossRefGoogle Scholar
  39. 39.
    P.I. Zamorano, A. Wigdorovitz, D.M.P. Filgueira, J.M. Escribano, A.M. Sadir, M.V. Borca, Vaccine 16, 558–563 (1998)CrossRefGoogle Scholar
  40. 40.
    Z.F. Liu, H.C. Chen, B. Wu, Acta Vet. Zootechnica Sin. 35, 70–73 (2004)CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Yannan He
    • 1
    • 2
  • Ping Qian
    • 1
    • 2
  • Keshan Zhang
    • 1
    • 2
  • Qingxia Yao
    • 1
    • 2
  • Dang Wang
    • 1
    • 2
  • Zhuofei Xu
    • 1
    • 2
  • Bin Wu
    • 1
    • 2
  • Meilin Jin
    • 1
    • 2
  • Shaobo Xiao
    • 1
    • 2
    Email author
  • Huanchun Chen
    • 1
    • 2
    Email author
  1. 1.State Key Laboratory of Agricultural MicrobiologyHuazhong Agricultural UniversityWuhanP.R. China
  2. 2.Laboratory of Animal Virology, College of Veterinary MedicineHuazhong Agricultural UniversityWuhanP.R. China

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