Virus Genes

, 35:147 | Cite as

Genetic and phenotypic differences between isolates of Murray Valley encephalitis virus in Western Australia, 1972–2003

  • Cheryl A. Johansen
  • Veronica Susai
  • Roy A. Hall
  • John S. Mackenzie
  • David C. Clark
  • Fiona J. May
  • Stéphane Hemmerter
  • David W. Smith
  • Annette K. Broom


Murray Valley encephalitis virus (MVEV) is a medically important mosquito-borne flavivirus found in Australia and Papua New Guinea (PNG). Partial envelope gene nucleotide sequences of 28 isolates of MVEV from Western Australia (WA) between 1972 and 2003 were aligned and compared phylogenetically with the prototype MVE-1-51 from Victoria in 1951 and isolates from northern Queensland and PNG. Monoclonal antibody-binding patterns were also investigated. Results showed that the majority of isolates of MVEV from widely disparate locations in WA were genetically and phenotypically homogeneous. Furthermore, isolates of MVEV from WA and northern Queensland were almost identical, confirming results from earlier studies. Recent isolates of MVEV from Western Province in PNG were more similar to Australian isolates of MVEV than to isolates from PNG in 1956 and 1966, providing further evidence for the movement of flaviviruses between PNG and Australia. Additional representatives of a unique variant of MVEV (OR156) from Kununurra in the northeast Kimberley region of WA were also detected. This suggests that the OR156 lineage is still intermittently active but may be restricted to a small geographic area in northern WA, possibly due to altered biological characteristics.


Murray Valley encephalitis virus Flavivirus Phylogenetic analysis Monoclonal antibody-binding pattern Virus movement 



The authors wish to acknowledge the assistance of Dr. Michael Lindsay, Susan Harrington, Adrian Stratico and Tony Wright (WA Department of Health), Brenda van Heuzen, Steven Crocker, Rosa Duthie, Maria Beilin, Margaret Wallace and Keryn Sturrock (The University of WA), Debra Nisbet and Dr. Andrew van den Hurk (The University of Queensland) and Dr. Scott Ritchie (Queensland Health) with mosquito collections and virus isolations. We also thank James Conlan and Dr. Helle Bielefeldt-Ohmann (The University of Queensland) for assistance with sequencing the isolates NG156 and MK6684. The late Professor Neville Stanley and Peter Liehne initiated and obtained funding for the early studies on MVEV in WA. We also thank Dr. Malcom Lawson (The University of WA) for helpful scientific discussions. This research was funded by the WA Department of Health.


  1. 1.
    J.S. Mackenzie, M.D. Lindsay, R.J. Coelen, A.K. Broom, R.A. Hall, D.W. Smith, Arch. Virol. 136, 447 (1994)PubMedCrossRefGoogle Scholar
  2. 2.
    I.D. Marshall, in The Arboviruses: Epidemiology and Ecology, ed. by T.P. Monath (CRC Press, Boca Raton, 1988), p. 151Google Scholar
  3. 3.
    A.K. Broom, M.D.A. Lindsay, S.A. Harrington, D.W. Smith, Vector Borne Zoonotic Dis. 2, 87 (2002)PubMedCrossRefGoogle Scholar
  4. 4.
    S.P. Cordova, D.W. Smith, A.K. Broom, M.D. Lindsay, G.K. Dowse, M.Y. Beers, Commun. Dis. Intell. 24, 368 (2000)PubMedGoogle Scholar
  5. 5.
    J.S. Mackenzie, D.W. Smith, A.K. Broom, M.R. Bucens, Medical J. Aust. 158, 591 (1993)Google Scholar
  6. 6.
    A.K. Broom, M.D.A. Lindsay, A.J. Plant, A.E. Wright, R.J. Condon, J.S. Mackenzie, Am. J. Trop. Med. Hyg. 67, 319 (2002)PubMedGoogle Scholar
  7. 7.
    R.A. Hall, A.K. Broom, A.C. Hartnett, M.J. Howard, J.S. Mackenzie, J. Virol. Methods 51, 201 (1995)PubMedCrossRefGoogle Scholar
  8. 8.
    A. Broom, K. Sturrock, B. van Heuzen, M. Lindsay, D. Smith, Arbovirus Res. Aust. 8, 43 (2001)Google Scholar
  9. 9.
    A.K. Broom, M.D.A. Lindsay, A.E. Wright, D.W. Smith, J.S. Mackenzie, Am. J. Trop. Med. Hyg. 69, 277 (2003)PubMedGoogle Scholar
  10. 10.
    H.F. Chapman, B.H. Kay, S.A. Ritchie, A. van den Hurk, J.M. Hughes, J. Med. Entomol. 37, 736 (2000)PubMedGoogle Scholar
  11. 11.
    C.A. Johansen, D.J. Nisbet, P. Zborowski, A.F. van den Hurk, S.A. Ritchie, J.S. Mackenzie, J. Am. Mosq. Control Assoc. 19, 392 (2003)PubMedGoogle Scholar
  12. 12.
    E.L. French Med. J. Aust. 1, 100 (1952)PubMedGoogle Scholar
  13. 13.
    C.A. Johansen, A.F. van den Hurk, S.A. Ritchie, P. Zborowski, D.J. Nisbet, R. Paru, M.J. Bockarie, J. Macdonald, A.C. Drew, T.I. Khromykh, J.S. Mackenzie, Am. J. Trop. Med. Hyg. 62, 631 (2000)PubMedGoogle Scholar
  14. 14.
    G.M. Woodroofe, I.D. Marshall Arboviruses from the Sepik district of New Guinea. Canberra: John Curtin School of Medical Research Annual Report, (Australian National University, 1971), pp. 90–91Google Scholar
  15. 15.
    A.T. Pyke, D.T. Williams, D.J. Nisbet, A.F. van den Hurk, C.T. Taylor, C.A. Johansen, J. Macdonald, R.A. Hall, R.J. Simmons, R.J.V. Mason, J.M. Lee, S.A. Ritchie, G.A. Smith, J.S. Mackenzie, Am. J. Trop. Med. Hyg. 65, 747 (2001)PubMedGoogle Scholar
  16. 16.
    J.H. Scherret, M. Poidinger, J.S. Mackenzie, A.K. Broom, V. Deubel, W.I. Lipkin, T. Briese, E.A. Gould, R.A. Hall, Emerg. Infect. Dis. 7, 697 (2001)PubMedGoogle Scholar
  17. 17.
    D.T. Williams, L.F. Wang, P.W. Daniels, J.S. Mackenzie, J. Gen. Virol. 81, 2471 (2000)PubMedGoogle Scholar
  18. 18.
    L.D. Kramer, L.J. Chandler, Arch. Virol. 146, 2431 (2001)CrossRefGoogle Scholar
  19. 19.
    F.J. May, M. Lobigs, E. Lee, D.J. Gendle, J.S. Mackenzie, A.K. Broom, J.V. Conlan, R.A. Hall, J. Gen. Virol. 87, 329 (2006)PubMedCrossRefGoogle Scholar
  20. 20.
    L. Dalgarno, D.W. Trent, H.H. Strauss, C.M. Rice, J. Mol. Biol. 187, 309 (1986)PubMedCrossRefGoogle Scholar
  21. 21.
    J.T. Roehrig, Adv. Virus Res. 59, 141 (2003)PubMedGoogle Scholar
  22. 22.
    P.C. McMinn, E. Lee, S. Hartley, J.T. Roehrig, L. Dalgarno, R.C. Weir, Virology 211, 10 (1995)PubMedCrossRefGoogle Scholar
  23. 23.
    M. Lobigs, I.D. Marshall, R.C. Weir, L. Dalgarno, Virology 165, 245 (1988)PubMedCrossRefGoogle Scholar
  24. 24.
    J.D. Thompson, D.G. Higgins, T.J. Gibson, Nucleic Acids Res. 22, 4673 (1994)PubMedCrossRefGoogle Scholar
  25. 25.
    S. Kumar, K. Tamura, M. Nei, Brief Bioinformatics 5, 150 (2004)PubMedCrossRefGoogle Scholar
  26. 26.
    D. Posada, K.A. Crandall, Bioinformatics 14, 817 (1998)PubMedCrossRefGoogle Scholar
  27. 27.
    D.L. Swofford PAUP*. Phylogenetic Analysis Using Parsimony (*and Other Methods). Version 4. Sinauer Associates, Sunderland, Massachusetts, (2005)Google Scholar
  28. 28.
    K. Tamura, M. Nei, Mol. Biol. Evol. 10, 512 (1993)PubMedGoogle Scholar
  29. 29.
    S. Guindon, O. Gascuel, Syst. Biol. 52, 696 (2003)PubMedCrossRefGoogle Scholar
  30. 30.
    N. Saitou, M. Nai, Mol. Biol. Evol. 4, 406 (1987)PubMedGoogle Scholar
  31. 31.
    J. Felsenstein, PHYLIP (Phylogeny Inference Package) Version 3.5c. Department of Genetics, University of Washington, Seattle. (1993)Google Scholar
  32. 32.
    A.K. Broom, R.A. Hall, C.A. Johansen, N. Oliveira, M.A. Howard, M.D. Lindsay, B.H. Kay, J.S. Mackenzie, Pathology 30, 286 (1998)PubMedCrossRefGoogle Scholar
  33. 33.
    S.C. Adams, A.K. Broom, L.M. Sammels, A.C. Hartnett, M.J. Howard, R.J. Coelen, J.S. Mackenzie, R.A. Hall, Virology 206, 49 (1995)PubMedCrossRefGoogle Scholar
  34. 34.
    R.A. Hall, G.W. Burgess, B.H. Kay, Immunol. Cell Biol. 66, 51 (1988)PubMedCrossRefGoogle Scholar
  35. 35.
    R.A. Hall, PhD Thesis. James Cook University, Townsville, Queensland, (1989)Google Scholar
  36. 36.
    E.A. Henchal, M.K. Gentry, J.M. McCown, W.E. Brandt, Am. J. Trop. Med. Hyg. 31, 830 (1982)PubMedGoogle Scholar
  37. 37.
    D.C. Clark, M. Lobigs, E. Lee, M.J. Howard, K. Clark, B.J. Blitvich, and R.A. Hall, J. Gen. Virol. 88, 1175 (2007)PubMedCrossRefGoogle Scholar
  38. 38.
    C.G. Liehne, S. Leivers, N.F. Stanley, M.P. Alpers, S. Paul, P.F.S. Liehne, K.H. Chan, Aust. J. Exp. Biol. Med. Sci. 54, 499 (1976)PubMedGoogle Scholar
  39. 39.
    J.A. Forbes, Murray Valley encephalitis 1974—also the epidemic variance since 1914 and predisposing rainfall patterns. (Australian Medical Publishing Co., Glebe, 1978)Google Scholar
  40. 40.
    N. Nicholls, Aust. J. Exp. Biol. Med. Sci. 64, 587 (1986)PubMedGoogle Scholar
  41. 41.
    R.J. Coelen, M.A. Lawson, L.M. Flynn, J.S. Mackenzie, Arbovirus Res. Aust. 5, 55 (1989)Google Scholar
  42. 42.
    M. Lobigs, I.D. Marshall, R.C. Weir, L. Dalgarno, Aust. J. Exp. Biol. Med. Sci. 64, 571 (1986)PubMedGoogle Scholar
  43. 43.
    J.A.R. Miles, D.W. Howes, Med. J. Aust. 1, 7 (1953)PubMedGoogle Scholar
  44. 44.
    B.H. Kay, R.A. Farrow, J. Med. Entomol. 37, 797 (2000)PubMedCrossRefGoogle Scholar
  45. 45.
    S.A. Ritchie, W. Rochester, Emerg. Infect. Dis. 7, 900 (2001)PubMedGoogle Scholar
  46. 46.
    R.J. Coelen, J.S. Mackenzie, J. Gen. Virol. 69, 1903 (1988)PubMedCrossRefGoogle Scholar
  47. 47.
    M.A. Lawson, PhD Thesis. The University of Western Australia, Nedlands, Perth, Western Australia (1988)Google Scholar
  48. 48.
    J.S. Mackenzie, M. Poidinger, D. Phillips, C. Johansen, R.A. Hall, J. Hanna, S. Ritchie, J. Shield, and R. Graham, in Factors in the emergence of arbovirus diseases. Ed. by J.F. Saluzzo, B. Dodet (Elsevier, Paris, 1997), pp. 191–136Google Scholar

Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Cheryl A. Johansen
    • 1
  • Veronica Susai
    • 1
  • Roy A. Hall
    • 2
  • John S. Mackenzie
    • 2
    • 3
  • David C. Clark
    • 2
  • Fiona J. May
    • 2
  • Stéphane Hemmerter
    • 4
  • David W. Smith
    • 1
    • 5
  • Annette K. Broom
    • 1
  1. 1.Discipline of Microbiology and Immunology, School of Biomedical, Biomolecular and Chemical SciencesThe University of Western AustraliaCrawleyAustralia
  2. 2.Department of Microbiology and Parasitology, School of Molecular and Microbial SciencesThe University of QueenslandSt. LuciaAustralia
  3. 3.Australian Biosecurity Collaborative Research CentreCurtin University of TechnologyPerthAustralia
  4. 4.Institute for the Biotechnology of Infectious DiseasesUniversity of TechnologySydneyAustralia
  5. 5.Western Australian Centre for Pathology and Medical ResearchNedlandsAustralia

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