Virus Genes

, Volume 34, Issue 2, pp 157–168 | Cite as

Genetic analysis of nonstructural genes (NS1 and NS2) of H9N2 and H5N1 viruses recently isolated in Israel

  • Caroline Banet-Noach
  • Alexander Panshin
  • Natalia Golender
  • Lubov Simanov
  • Ezra Rozenblut
  • Shimon Pokamunski
  • Michael Pirak
  • Yevgenii Tendler
  • Maricarmen García
  • Boris Gelman
  • Ruslan Pasternak
  • Shimon Perk
Original Article

Abstract

The avian influenza virus subtype H9N2 affects wild birds, domestic poultry, swine, and humans; it has circulated amongst domestic poultry in Israel during the last 6 years. The H5N1 virus was recorded in Israel for the first time in March 2006. Nonstructural (NS) genes and NS proteins are important in the life cycle of the avian influenza viruses. In the present study, NS genes of 21 examples of H9N2 and of two examples of H5N1 avian influenza viruses, isolated in Israel during 2000–2006, were completely sequenced and phylogenetically analyzed. All the H9N2 isolates fell into a single group that, in turn, was subdivided into three subgroups in accordance with the time of isolation; their NS1 and NS2 proteins possessed 230 and 121 amino acids, respectively. The NS1 protein of the H5N1 isolates had five amino acid deletions, which was typical of highly pathogenic H5N1 viruses isolated in various countries during 2005–2006. Comparative analysis showed that the NS proteins of the H9N2 Israeli isolates contained few amino acid sequences associated with high pathogenicity or human host specificity.

Keywords

Avian influenza virus Genetic analysis H9N2 H5N1 NS genes 

Notes

Acknowledgements

We thank Mrs. Rivi Ascarelli-Goell, MSc and Mrs. Magda David, MSc, and Mrs. Faina Golender for excellent technical assistance. This study was supported by the Kameya Grant of the Israel Ministry of Absorption, by Research Grant No. IS-3679–05 from BARD, the United States-Israel Binational Agricultural Research and Development Fund, and by Grant No. 847–0319 from the Poultry Association of the Israel Ministry of Agriculture and Rural Development.

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Copyright information

© Springer Science+Business Media, LLC 2006

Authors and Affiliations

  • Caroline Banet-Noach
    • 1
  • Alexander Panshin
    • 1
  • Natalia Golender
    • 1
  • Lubov Simanov
    • 1
  • Ezra Rozenblut
    • 1
  • Shimon Pokamunski
    • 2
  • Michael Pirak
    • 2
  • Yevgenii Tendler
    • 3
  • Maricarmen García
    • 4
  • Boris Gelman
    • 1
  • Ruslan Pasternak
    • 1
  • Shimon Perk
    • 1
  1. 1.Division of Avian and Aquatic DiseasesKimron Veterinary InstituteBeit DaganIsrael
  2. 2.Poultry Veterinary ServicesBeit DaganIsrael
  3. 3.Rambam Medical CenterHaifaIsrael
  4. 4.Poultry Diagnostic and Research CenterCollege of Veterinary Medicine, The University of GeorgiaAthensUSA

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