Virus Genes

, Volume 35, Issue 2, pp 235–242

Sequence and phylogenetic analysis of the S1 genome segment of turkey-origin reoviruses

  • J. Michael Day
  • Mary J. Pantin-Jackwood
  • Erica Spackman
Original Article


Based on previous reports characterizing the turkey-origin avian reovirus (TRV) σB (σ2) major outer capsid protein gene, the TRVs may represent a new group within the fusogenic orthoreoviruses. However, no sequence data from other TRV genes or genome segments has been reported. The σC protein encoded by the avian reovirus S1 genome segment is the cell attachment protein and a major antigenic determinant for avian reovirus. The chicken reovirus S1 genome segment is well characterized and is well conserved in viruses from that species. This report details the amplification, cloning and sequencing of the entire S1 genome segment from two and the entire coding sequences of the σC, p10 and p17 genes from an additional five TRVs. Sequence analysis reveals that of the three proteins encoded by the TRV S1 genome segment, σC shares at most 57% amino acid identity with σC from the chicken reovirus reference strain S1133, while the most similar p10 and p17 proteins share 72% and 61% identity, respectively, with the corresponding S1133 proteins. The most closely related mammalian reovirus, the fusogenic Nelson Bay reovirus, encodes a σC protein that shares from 25% to 28% amino acid identity with the TRV σC proteins. This report supports the earlier suggestion that the TRVs are a separate virus species within the Orthoreovirus genus, and may provide some insight into TRV host specificity and pathogenesis.


Orthoreovirus Turkey reovirus p10 p17 SigmaC 


  1. 1.
    M. Shmulevitz, Z. Yameen, S. Dawe, J. Shou, D. O’Hara, I. Holmes, R. Duncan, J. Virol. 76, 609 (2002)PubMedCrossRefGoogle Scholar
  2. 2.
    J.K. Rosenberger, in Diseases of Poultry 11th edn., ed. by Y.M. Saif (Blackwell, Ames, IA, 2003), pp. 283–298Google Scholar
  3. 3.
    D.R. Kapczynski, H.S. Sellers, V. Simmons, S. Schultz-Cherry, Virus Genes 25, 95 (2002)PubMedCrossRefGoogle Scholar
  4. 4.
    H.S. Sellers, E.G. Linnemann, L. Pereira, D.R. Kapczynski, Avian Dis. 48, 651 (2004)PubMedCrossRefGoogle Scholar
  5. 5.
    H.J. Barnes, J.S. Guy, in Diseases of poultry, 11th edn., ed. by Y.M Saif, et al., (Blackwell, Ames, IA, 2003), pp. 1171–1180Google Scholar
  6. 6.
    E. Spackman, M.J. Pantin-Jackwood, J.M. Day, H.S. Sellers, Avian Path 34, 291 (2005)CrossRefGoogle Scholar
  7. 7.
    P. Guardado Calvo, G.C. Fox, X.L. Hermo Parrado, A.L. Llamas-Saiz, C. Costas, J. Martinez-Costas, J. Benavente, M.J. van Raaij, J. Mol. Biol. 354, 137 (2005)PubMedCrossRefGoogle Scholar
  8. 8.
    M.J. van Raaij, X.L. Hermo Parrado, P. Guardado Calvo, G.C. Fox, A.L. Llamas-Saiz, C. Costas, J. Martinez-Costas, J. Benavente, Acta Crystallog. Sect F 61, 651 (2005)CrossRefGoogle Scholar
  9. 9.
    M. Shmulevitz, R. Duncan, EMBO J. 19, 902 (2000)PubMedCrossRefGoogle Scholar
  10. 10.
    C. Costas, J. Martinez-Costas, G. Boledon, J. Benavente, J. Virol. 79, 2141 (2005)PubMedCrossRefGoogle Scholar
  11. 11.
    A. Kant, F. Balk, L. Born, D. Van Roozelaar, J. Heijmans, A. Gielkens, A. Ter Huurne, Vet. Res. 34, 203 (2003)PubMedCrossRefGoogle Scholar
  12. 12.
    J.K. Rosenberger, F.J. Sterner, S. Botts, K.P. Lee, A. Margolin, Avian Dis. 33, 535 (1989)PubMedCrossRefGoogle Scholar
  13. 13.
    R. Duncan, F.A. Murphy, R.R. Mirkovic, Virology 212, 752 (1995)PubMedCrossRefGoogle Scholar
  14. 14.
    R. Duncan, J. Corcoran, J. Shou, D. Stoltz, Virology 319, 131 (2004)PubMedCrossRefGoogle Scholar
  15. 15.
    G. Gard, R.W. Compans, J. Virol. 6, 100 (1970)PubMedGoogle Scholar
  16. 16.
    Hung-Jen Liu, J.J. Giambrone, J. Virol. Methods 63, 203 (1997)PubMedCrossRefGoogle Scholar
  17. 17.
    M.L. Nibert, L.A. Schiff, in Fields Virology, 4th edn., ed. by D.M. Knipe, P.M. Howley, (Williams & Wilkins, Lippincott, 2001), pp. 1679–1728Google Scholar
  18. 18.
    Y. Vasserman, D. Eliahoo, E. Hemsani, N. Kass, G. Ayali, S. Pokamunski, J. Pitcovski, Avian Diseases 48, 271 (2004)PubMedCrossRefGoogle Scholar
  19. 19.
    R. Bassel-Duby, A. Jayasuriya, D. Chatterjee, N. Sonenberg, J.V. Maizel Jr., Nature 315, 421 (1985)PubMedCrossRefGoogle Scholar
  20. 20.
    R. Duncan, D. Horne, L.W. Cashdollar, W.K. Joklik, P.W.K. Lee, Virology 174, 399 (1990)PubMedCrossRefGoogle Scholar
  21. 21.
    G. Leone, D.C.W. Mah, P.W.K. Lee, Virology 182, 346 (1991)PubMedCrossRefGoogle Scholar
  22. 22.
    G. Leone, R. Duncan, D.C.W. Mah, A. Price, L.W. Cashdollar, P.W.K. Lee, Virology 182, 336 (1990)CrossRefGoogle Scholar
  23. 23.
    E. Spackman, D. Kapczynski, H. Sellers, Avian Diseases 49, 86 (2005)PubMedCrossRefGoogle Scholar
  24. 24.
    B.L. Haller, M.L. Barkon, G.P. Volger, H.W. Virgin, J. Virol. 69, 357 (1995)PubMedGoogle Scholar
  25. 25.
    K.M. Kaye, D.R. Spriggs, R. Bassell-Duby, B.N. Fields, K.L. Tyler, J. Virol. 59, 90 (1986)PubMedGoogle Scholar
  26. 26.
    D.R. Spriggs, R.T. Bronson, B.N. Fields, Science 220, 505 (1983)PubMedCrossRefGoogle Scholar
  27. 27.
    D.E. Roessler, J.K. Rosenberger, In vitro and in vivo characterization of avian reoviruses. III. Host factors affecting virulence and persistence. Avian Dis. 33, 555 (1989)PubMedCrossRefGoogle Scholar
  28. 28.
    H.W. Hsu, H.Y. Su, P.H. Huang, L.H. Lee, H.J. Liu, Avian Dis 49, 36 (2005)PubMedCrossRefGoogle Scholar
  29. 29.
    R. Duncan, Virology 260, 316 (1999)PubMedCrossRefGoogle Scholar
  30. 30.
    J.D. Thompson, D.G. Higgins,T.J. Gibson, Nucleic Acids Res. 22, 4673 (1994)PubMedCrossRefGoogle Scholar
  31. 31.
    J.D. Thompson, T.J. Gibson, F. Plewniak, F. Jeanmougin, D.G. Higgins, Nucleic Acids Res. 24, 4876 (1997)CrossRefGoogle Scholar
  32. 32.
    D.L. Swofford, PAUP*. Phylogenetic Analysis Using Parsimony (*and other methods). Version 4 (Sinauer Associates, Sunderland, MA, 2002)Google Scholar

Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • J. Michael Day
    • 1
  • Mary J. Pantin-Jackwood
    • 1
  • Erica Spackman
    • 1
  1. 1.Southeast Poultry Research LaboratoryUSDA-ARSAthensUSA

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