To define the function of the GDD motif of the RNA-dependent RNA polymerase (RdRp) of classical swine fever virus (CSFV), single amino acid substitutions were introduced into the CSFV NS5B. All substitutions within the GDD motif were detrimental to the polymerase activity, the binding activity and the terminal nucleotidyl transferase activity of the NS5B protein. It was also found that the wild-type NS5B had higher RdRp activity with Mg+2 than with Mn+2 whereas some mutants worked better with Mn+2 than with Mg+2, suggesting that substitutions within the GDD motif modified the enzyme cation preferences and the GDD sequence of CSFV NS5B might be involved in polymerase–metal interaction. Therefore, the GDD amino acid sequence is important for the function of CSFV RdRp.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
E.K. O’Reilly, C.C. Kao, Virology 252, 287–303 (1998)
V. Lohmann, F. Körner, U. Herian, R. Bartenschlager, J. Virol. 71, 8416–8428 (1997)
M. Xiao, H. Li, Y. Wang, X. Wang, W. Wang, J. Peng, J. Chen, B. Li, J. Gen. Virol. 87, 347–356 (2006)
S.A. Jablonski, C.D. Morrow, J. Virol. 69, 1532–1539 (1995)
A. L´opez V´azquez, J.M. Martin Alonso, F. Parra, J. Virol. 74, 3888–3891 (2000)
V.C.H. Lai, C.C. Kao, E. Ferari, J. Prak, A. Uss, J. Wright-Minogue, Z. Hong, J.Y.N. Lau, J. Virol. 73, 10129–10136 (1999)
D. Brown, L. Gold, Proc. Natl. Acad. Sci. 93, 11558–11562 (1996)
M. Xiao, Y. Wang, J. Chen, B. Li, Virus Genes 7, 67–74 (2003)
M. Xiao, J. Gao, W. Wang, Y. Wang, J. Chen, J. Chen, B. Li, Eur. J. Biochem. 271, 3888–3896 (2004)
S.A. Jablonski, M. Luo, C.D. Morrow, J. Virol. 65, 4565–4572 (1991)
This work was supported by National Natural Science Foundation of China (30170214) and by Science and Technology Foundation of Shanghai Higher Education.
About this article
Cite this article
Wang, Y., Xiao, M., Chen, J. et al. Mutational analysis of the GDD sequence motif of classical swine fever virus RNA-dependent RNA polymerases. Virus Genes 34, 63–65 (2007). https://doi.org/10.1007/s11262-006-0001-z
- Classical swine fever virus
- RNA-dependent RNA polymerase
- GDD motif