Advertisement

Virus Genes

, Volume 32, Issue 2, pp 177–186 | Cite as

Searching for a New Putative Cryptic Virus in Pinus sylvestris L

  • Dorina Veliceasa
  • Natalya Enünlü
  • Péter B. Kós
  • Sigrid Köster
  • Eckhardt Beuther
  • Bogdan Morgun
  • Sachin D. Deshmukh
  • Noémi LukácsEmail author
Original Article

Abstract

Double-stranded RNAs (dsRNAs) were detected in different pine populations in Germany and Hungary. Two dsRNA species of 1.5 and 1.58 kbp, respectively, persisted in the same trees for at least 2 years and their presence was not associated with any symptoms. The dsRNAs were found to sediment in the VLP (virus-like particles) fraction and to be protected by protein(s) against RNase A digestion at low salt. cDNA cloning and sequencing of the smaller segment (dsRNA2) led to the identification of a putative RNA-dependent RNA-polymerase (RdRp) containing the GDD, as well as three other, conserved motifs. Sequence comparison with different RNA viruses and phylogenetic analysis indicates that the putative RdRp from pine shows highest similarity to the homologous proteins of Beet cryptic virus 3 and of a cryptic virus of Pyrus pyrifolia. On the basis of these results we suggest that the 1.5 and 1.58 kbp dsRNAs in P. sylvestris may represent the genomic segments of a new plant cryptic virus, Cryptoviruses have not yet been reported to occur in Gymnosperms.

Keywords

Cryptovirus dsRNA Pinus sylvestris Partitiviridae RNA-dependent RNA polymerase 

Notes

Acknowledgements

This project was supported by Grants OTKA T032861 and DFG Lu 386/1–2. We thank Drs. I. Szabó and S. Bogya for providing us with pine samples and Dr. L. Vajna for advise on endophytic fungi. We are grateful to M. Kutzner, E. Bárdosi and G. Fleit for technical help and to Dr. P. Symmons and E. Bába for helping us with the manuscript.

References

  1. 1.
    G.G. Brown, P.M. Finnegan, Int. Rev. Cytol. 117, 1–56 (1989)PubMedGoogle Scholar
  2. 2.
    H. Ishihara, J.Y. Pak, T. Fukuhara, T. Nitta, Planta 187, 475–482 (1992)CrossRefGoogle Scholar
  3. 3.
    A.L. Wang, C.C. Wang, Annu. Rev. Microbiol. 45, 251–263 (1991)CrossRefPubMedGoogle Scholar
  4. 4.
    S. Miyazaki, K. Iwabuchi, J.Y. Pak, T. Fukuhara, T. Nitta, Insect Biochem. Mol. Biol. 26, 955–961 (1996)Google Scholar
  5. 5.
    D. Jiang, S.A. Ghabrial, J. Gen. Virol. 85, 2111–2121 (2004)CrossRefPubMedGoogle Scholar
  6. 6.
    G. Boccardo, V. Lisa, E. Luisoni, R.G. Milne, Adv. Virus Res. 32, 171–214 (1987)PubMedGoogle Scholar
  7. 7.
    G. Boccardo, T. Candresse, Arch. Virol. 150, 399–402 (2005)PubMedGoogle Scholar
  8. 8.
    G. Boccardo, T. Candresse, Arch. Virol. 150, 403–405 (2005)PubMedGoogle Scholar
  9. 9.
    J. Schönborn, J. Oberstraβ, E. Breyel, J. Tittgen, J. Schumacher, N. Lukács, Nucleic Acids Res. 19, 2993–3000 (1991)PubMedGoogle Scholar
  10. 10.
    N. Lukács, J. Virol. Methods 47, 255–272 (1994)PubMedGoogle Scholar
  11. 11.
    A.M. Pirtilla, H. Pospiech, H. Laukkanen, R. Myllyla, A. Hohtola, Microb. Ecol. 45(1), 53–62 (2003)Google Scholar
  12. 12.
    B. Ziegenhagen, P. Guillemaut, E. Scholz, Plant Mol. Biol. Rep. 11, 117–121 (1993)Google Scholar
  13. 13.
    J.A. Dodds, T.J. Morris, R.L. Jordan, Annu. Rev. Phytopathol. 22, 151–168 (1984)CrossRefGoogle Scholar
  14. 14.
    J. Sambrook, E.F. Fritsch, T. Maniatis, Molecular Cloning: A Laboratory Manual, 2nd edn. (Cold Spring Harbor, Cold Spring Harbor Laboratory, NY, 1989)Google Scholar
  15. 15.
    M. Flachmann, Ph.D. thesis, University Hohenheim, (1992), pp. 83–85Google Scholar
  16. 16.
    P. Froussard, Nucleic Acids Res. 20, 2900 (1992)PubMedGoogle Scholar
  17. 17.
    Y.G. Choi, B.J. Croft, J.W. Randles, Phytopathology 89, 877–883 (1999)Google Scholar
  18. 18.
    S.F. Altschul, T.L. Madden, A.A. Schäffer, J.H. Zhang, Z. Zhang, W. Miller, D.J. Lipman, Nucleic Acids Res. 25, 3389–3402 (1997)CrossRefPubMedGoogle Scholar
  19. 19.
    J.D. Thompson, T.J. Gibson, F. Plewniak, F. Jeanmougin, D.G. Higgins, Nucleic Acids Res. 25, 4876–4882 (1997)CrossRefPubMedGoogle Scholar
  20. 20.
    N. Saitou, M. Nei, Mol. Biol. Evol. 4, 406–425 (1987)PubMedGoogle Scholar
  21. 21.
    G. Perriere, M. Gouy, Biochimie 78, 364–369 (1996)PubMedGoogle Scholar
  22. 22.
    O. Poch, I. Sauvaget, M. Delarue, N. Tordo, EMBO J. 8, 3867–3874 (1989)PubMedGoogle Scholar
  23. 23.
    H. Osaki, A. Kudo, Y. Ohtsu, Biosci. Biotechnol. Biochem. 62, 2101–2106 (1998)CrossRefPubMedGoogle Scholar
  24. 24.
    W.S. Xie, J.F. Antoniw, R.F. White, J. Gen. Virol. 74, 1467–1470 (1993)PubMedGoogle Scholar
  25. 25.
    M. Nogawa, T. Kageyama, A. Nakatani, G. Taguchi, M. Shimosaka, M. Okazaki, Biosci. Biotechnol. Biochem. 60, 784–788 (1996)PubMedGoogle Scholar
  26. 26.
    R. Rong, S. Rao, S.W. Scott, G.R. Carner, F.H. Tainter, Virus Res. 90, 217–224 (2002)CrossRefPubMedGoogle Scholar
  27. 27.
    T.T. Tuomivirta, J. Hantula, Arch. Virol. 148, 2293–2305 (2003)PubMedGoogle Scholar
  28. 28.
    T.R. Van der Lende, E.H. Cuitman, M.G. Gunnejwijk, L. Yu, J.G. Wessels, Virology 217(1), 88–96 (1996)PubMedGoogle Scholar
  29. 29.
    C.S. Oh, B.I. Hillman, J. Gen. Virol. 76, 1461–1470 (1995)PubMedGoogle Scholar
  30. 30.
    L. Covelli, R.H. Coutts, F. Di Serio, A. Citir, S. Acikgoz, C. Hernandez, A. Ragozzino, R.J. Flores, Gen. Virol. 85, 3389–3397 (2004)Google Scholar
  31. 31.
    R.H. Coutts, L. Covelli, F. Di Serio, A. Citir, S. Acikgoz, C. Hernandez, A. Ragozzino, R. Flores, J. Gen. Virol. 85, 3399–4403 (2004)CrossRefPubMedGoogle Scholar
  32. 32.
    N.V. Khramtsov, K.M. Woods, M.V. Nesterenko, C.C. Dykstra, S.J. Upton, Mol. Microbiol. 26, 289–300 (1997)CrossRefPubMedGoogle Scholar
  33. 33.
    P. Compel, I. Papp, M. Bibo, C. Fekete, L. Hornok, Virus Genes 18, 49–56 (1999)CrossRefPubMedGoogle Scholar
  34. 34.
    H. Osaki, K. Nomura, T. Iwanami, S. Kanematsu, I. Okabe, N. Matsumoto, A. Sasaki, Y. Ohtsu, Tanaka Virus Genes 25, 139–145 (2002)Google Scholar
  35. 35.
    H. Osaki, K. Nomura, N. Matsumoto, Y. Ohtsu, Mycol. Res. 108, 635–640 (2004)CrossRefPubMedGoogle Scholar
  36. 36.
    S. Huang, S.A. Ghabrial, Proc. Natl. Acad. Sci. USA 93, 12541–12546 (1996)PubMedGoogle Scholar
  37. 37.
    K. Ihrmark, J. Zheng, E. Stenstrom, J. Stenlid, For. Pathol. 31, 387–394 (2001)Google Scholar
  38. 38.
    K. Ihrmark, Ph.D. thesis, Swedish University of Agricultural sciences, Uppsala, Sweden, (2001)Google Scholar
  39. 39.
    K.D. Stuart, R. Weeks, L. Guilbride, P.J. Myler, Proc. Natl. Acad. Sci. USA 89, 8596–8600 (1992)PubMedGoogle Scholar
  40. 40.
    S.M. Scheffter, Y.T. Ro, I.K. Chung, J.L. Patterson, Virology 212, 84–90 (1995)CrossRefPubMedGoogle Scholar
  41. 41.
    I.P. Ahn, Y.H. Lee, Mol. Plant Microbe. Interact. 14, 496–507 (2001)PubMedGoogle Scholar
  42. 42.
    J.W. Kim, S.Y. Kim, K.M. Kim, Virus Genes 27, 249–256 (2003)PubMedGoogle Scholar
  43. 43.
    E.E. Strauss, D.K. Lakshman, S.M. Tavantzis, J. Gen. Virol. 81, 549–555 (2000)PubMedGoogle Scholar
  44. 44.
    T. Icho, R.B. Wickner, J. Biol. Chem. 264, 6716–6723 (1989)PubMedGoogle Scholar
  45. 45.
    O. Preisig, B.D. Wingfield, M.J. Wingfield, Virology 252, 399–406 (1998)CrossRefPubMedGoogle Scholar
  46. 46.
    J.H. Tai, C.F. Ip, Virology 206, 773–776 (1995)CrossRefPubMedGoogle Scholar
  47. 47.
    J.A. Bruenn, Nucleic Acids Res. 21, 5667–5669 (1993)PubMedGoogle Scholar
  48. 48.
    S.A. Ghabrial, K.W. Buck, B.I. Hillman, R.G. Milne, in Eighth Report of the International Committee on Taxonomy of Viruses. ed. by C.M. Fauquet, M.A. Mayo, J. Maniloff, U. Desselberger, A.L. Ball (Elsevier/Academic press, London, 2005)Google Scholar
  49. 49.
    N. Enünlü, D. Veliceasa, S. Deshmukh, B. Morgun, V. Stepanyuk, S. Köster, N. Lukács, in 8th International Congress in Plant Pathology, Christchurch, New Zealand, 2–7 February 2003, A17.6Google Scholar
  50. 50.
    S.A. Ghabrial, Virus Genes 16, 119–131 (1998)CrossRefPubMedGoogle Scholar
  51. 51.
    S.H. Faeth, Oikos 98, 25–36 (2002)CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • Dorina Veliceasa
    • 1
  • Natalya Enünlü
    • 1
  • Péter B. Kós
    • 1
  • Sigrid Köster
    • 2
  • Eckhardt Beuther
    • 2
  • Bogdan Morgun
    • 1
  • Sachin D. Deshmukh
    • 1
  • Noémi Lukács
    • 1
    • 2
    • 3
    Email author
  1. 1.Biological Research CenterInstitute of Plant BiologySzegedHungary
  2. 2.Institute of Physical BiologyHeinrich Heine UniversityDüsseldorfFRG
  3. 3.Department of Plant Physiology and Plant BiochemistryCorvinus University BudapestBudapestHungary

Personalised recommendations