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Analysis of the Molecular Variability of Grapevine Leafroll-associated Virus 1 Reveals the Presence of Two Distinct Virus Groups and their Mixed Occurrence in Grapevines

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Abstract

Genetic diversity of eight Grapevine leafroll-associated virus 1 (GLRaV-1) isolates recovered from grapevines in three distinct locations in the Czech Republic and Slovakia was characterised by restriction fragment length polymorphism (RFLP) analysis and by sequencing of cloned 540 bp fragment of the heat shock protein 70 (HSP70) gene. Comparison and phylogenetic analysis of obtained and previously available sequence data revealed the existence of two groups of GLRaV-l isolates, tentatively named A and E (genetic divergence between A and E group reached 13.9%). An RT-PCR detection method followed by simple restriction analysis was developed, showing the potential to differentiate GLRaV-1 isolates of these groups. Interestingly, a mixed infection of two GLRaV-1 groups in the same plant was frequently detected together with a high intra-group variability in some isolates.

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Author information

Correspondence to Petr Komínek.

Additional information

The sequences reported in this work have been deposited in the Genbank database and assigned the accession numbers AY754905 to AY754944.

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Komínek, P., Glasa, M. & Bryxiová, M. Analysis of the Molecular Variability of Grapevine Leafroll-associated Virus 1 Reveals the Presence of Two Distinct Virus Groups and their Mixed Occurrence in Grapevines. Virus Genes 31, 247–255 (2005). https://doi.org/10.1007/s11262-005-3236-1

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Key words

  • ampelovirus
  • diagnosis
  • HSP70
  • mixed infection
  • restriction analysis
  • sequence diversity