Virus Genes

, Volume 33, Issue 1, pp 51–58

Murine Gammaherpesvirus (MHV) MK3 Gene Sequence Diversity among 72, 4556, and 68 Strains

  • Moniká Valovičová
  • Ingeborg Režuchorá
  • Katarína Mačáková
  • Petra Petrová
  • Ján Matis
  • Marcela Kúdelová
Original Article

Abstract

Murid herpesvirus 4 (MuHV-4) currently serves as a model for study of human gamma-herpesvirus pathogenesis. It codes for MK3 protein that similarly as K5 protein of Kaposi’s sarcoma-associated herpesvirus are members of a family of structurally related viral immune evasion molecules possessing RING-CH finger domain with ubiquitin ligase activity. Murine herpesvirus 72 (MHV-72) isolated from the same species of free-living small rodent is considered as closely related to Murine herpesvirus 68 (MHV-68). Studies on MHV-72, identified dissimilarity from MHV-68 in the sequence of glycoprotein 150 [K. Mačáková, J. Matis, I. Režuchová, O. Kúdela, H. Rašlová, M. Kúdelová, Virus Genes 26, 89–95 (2003)]. Murine herpesvirus 4556 (MHV-4556) is relatively new, till now, uncharacterised strain isolated from different murid species Apodemus flavicollis. We have therefore sequenced the MK3 gene of MHW-72 as well as of MHV-4556 to find out the evidence of their difference from that of MHV-68. We show here the unique nucleotide mutation in MHV-72 MK3 gene changing the codon at C-end of MK3 protein that was earlier predicted to function in interaction with TAP1/2. Furthermore, one from two nucleotide mutations found for MHV-4556 MK3 gene changed the codon that is localized at N-terminus of MK3 protein. MHV-4556-specific mutation was found within MK3 RING-CH finger domain known to be necessary for the ubiquitination of MHC class I proteins. Moreover, the latter established the new restriction site specific for MHV-4556.

Keywords

Murine gammaherpesvirus MHV-72 strain MHV-4556 strain MK3 Sequence analysis Gene diversity 

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Copyright information

© Springer Science+Business Media, LLC 2006

Authors and Affiliations

  • Moniká Valovičová
    • 1
  • Ingeborg Režuchorá
    • 1
  • Katarína Mačáková
    • 1
  • Petra Petrová
    • 1
  • Ján Matis
    • 1
  • Marcela Kúdelová
    • 1
  1. 1.Institute of VirologySlovak Academy of SciencesBratislavaSlovak Republic

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