Virus Genes

, Volume 30, Issue 3, pp 383–393 | Cite as

Molecular Phylogenetic Analysis of Iranian HDV Complete Genome

  • Farida Behzadian
  • Farzaneh SabahiEmail author
  • Mohsen Karimi
  • Majid Sadeghizadeh
  • Nader Maghsoudi
  • Ramin Sarrami Forooshani
  • Leila Shahinsaz


Hepatitis D (delta) virus (HDV) is a subviral pathogen agent and a satellite of Hepatitis B virus. Three distinct genotypes are described for HDV; genotype I is distributed worldwide but other genotypes appear to be more restricted geographically. In the present study, the entire nucleotide sequence of an HDV isolate from an Iranian patient (IR-1) was obtained using twelve pairs of primers to amplify six overlapping fragments covering the whole HDV genome by RT-nested PCR. Phylogenetic and pairwise alignments were done on this new isolate to determine IR-1 position among other isolates. Our results indicate that IR-1 contains 1676 nucleotides encoding 214 a.a. of the hepatitis delta antigen (HDAg). This new isolate belongs to genotype I with most sequence similarity to an Italian HDV isolate (92.6%). At amino acid level, predicted HDAg sequence of IR-1 revealed the most homology with those of Italian and Lebanese isolates. Data analysis confirmed genetic variability and heterogeneity of the HDV species isolated from different geographical areas.

Key words

alignment complete genome sequence hepatitis D virus Iranian phylogenetic analysis RT-PCR 


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Copyright information

© Springer Science+Business Media, Inc. 2005

Authors and Affiliations

  • Farida Behzadian
    • 1
  • Farzaneh Sabahi
    • 1
    Email author
  • Mohsen Karimi
    • 2
  • Majid Sadeghizadeh
    • 3
  • Nader Maghsoudi
    • 3
  • Ramin Sarrami Forooshani
    • 2
  • Leila Shahinsaz
    • 1
  1. 1.Department of Virology, School of Medical Sciences, Department of Genetics, School of Basic SciencesTarbiat Modares UniversityTehranIran
  2. 2.Department of BiotechnologyPasetur InstituteTehranIran
  3. 3.Neuroscience Research CenterSchool of Medical Sciences, Shaheed Beheshti UniversityTehranIran

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