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Virus Genes

, Volume 30, Issue 2, pp 139–150 | Cite as

The Function of Envelope Protein P74 from Autographa californica Multiple Nucleopolyhedrovirus in Primary Infection to Host

  • Wenke Zhou
  • Lunguang Yao
  • Hua Xu
  • Feng Yan
  • Yipeng QiEmail author
Article

Abstract

This research investigated the function of envelope protein P74 of Autographa californica multiple nuclear polyhedrosis virus (AcMNPV) in primary infection to host. A p74-inactivation recombinant baculovirus, rAc-gfpΔ p74, was constructed by inserting gfp driven by AcMNPV polyhedrin promoter into the p74 locus of AcMNPV genome. Bioassays showed that the P74-null occlusion bodies (OBs) failed to infect its natural host larvae, Spodoptera exigua, per os, while the p74-null budded virus (BVs) could infect host larvae by injection. However, its inability for oral infectivity was rescued by a mixed infection with wild-type OBs or with the purified P74 protein expressed in Spodoptera frugiperda Sf-9 cells, and the P74 protein rescue was in a dosage-dependent manner. The 50% lethal dosage (LD50) value of a P74 overexpression recombinant virus, rAc-p74++-polh+, which contained two copies of p74 gene, was not significantly different from that of wild-type virus. One-step growth curve assays of viruses suggested that BV production from cells infected with p74-null virus was similar to that from cells infected with wild-type virus or the P74 overexpression virus. ELISA analysis indicated that P74 protein could bind its host brush border membrane vesicles (BBMV) efficiently with saturation, but it could only bind its sensitive midgut BBMV specifically. In vitro pull-down assay showed that a protein of approximately 35 kDa in the BBMV was involved in the specific binding. These results demonstrated that the P74 protein is essential for oral infectivity of occlusion-derived virus (ODV) and plays a role in midgut attachment and fusion.

Keywords

AcMNPV baculovirus p74 gene BBMV oral infectivity primary infection 

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References

  1. Blissard G., Black B., Crook N., Keddie B.A., Possee R., Rohrmann G., Theilmann D., and Volkman L.E. In M.H.V. van Regenmortel et al. (eds.), Virus Taxonomy: Classification and Nomenclature of Viruses. Seventh report of the International Committee for the Taxonomy of Viruses. Academic Press, San Diego, Calif. 2000, pp. 195–202.Google Scholar
  2. Blissard, G.W. 1996Cytotechnology207393Google Scholar
  3. Miller L.K., The Baculoviruses, Plenum Press, New York. 1997.Google Scholar
  4. Rohrmann, G.F. 1992J Gen Virol73749761Google Scholar
  5. Bischoff, D.S., Slavicek, J.M. 1997J Virol7181338140Google Scholar
  6. Tanada, Y., Invertebr, J 1985Pathol45125138Google Scholar
  7. Wang,  P., Hammer, D.A., Granados, R.R. 1994J Gen Virol7519611967Google Scholar
  8. Granados, R.R., Lawler, K.A. 1981Virology108297308Google Scholar
  9. Horton, H.M., Burand, J.P. 1993J Virol6718601868Google Scholar
  10. Engelhard,  E.K., Kam-Morgan, L.N.W., Washburn, J.O., Volkman, L.E. 1994Proc Natl Acad Sci USA9132243227Google Scholar
  11. Braunagel, S.C., Summers, M.D. 1994Virology202315328Google Scholar
  12. Volkman, L.E., Goldsmith, P.A. 1985Virology143185195Google Scholar
  13. Volkman, L.E., Goldsmith, P.A., Hess, R.T. 1986Virology148288297Google Scholar
  14. Granados, R.R. 1978Virology90170174Google Scholar
  15. Summers, M.D. 1971J. Ultrastruct. Res.35606625Google Scholar
  16. Herniou, E.A., Luque, T., Chen, X., Vlak, J.M., Winstanley, D., Cory, J.S., O’Reilly, D.R. 2001J. Virol7581178126Google Scholar
  17. Zanotto, P.M., Kessing, B.D., Maruniak, J.E. 1993J Invertebr.Pathol62147164Google Scholar
  18. Hefferon, K.L., Oomens, A.G.P., Monsma, S.A., Finnerty, C.M., Blissard, G.W. 1999Virology258455468Google Scholar
  19. Blissard, G.W., Wenz, J.R. 1992J Virol6668296835Google Scholar
  20. Monsma, S.A., Blissard, G.W. 1995J Virol6925832595Google Scholar
  21. IJkel, W.F.J., Strien, E.A., Heldens, J.G., Broer, R., Zuidema, D., Goldbach, R.W., Vlak, J.M. 1999J Gen Virol8032893304Google Scholar
  22. Kuzio, J., Pearson, M.N., Harwood, S.H., Funk, C.J., Evans, J.T., Slavicek, J.M., Rohrmann, G.F. 1999Virology2531734Google Scholar
  23. IJkel, W.F.J., Westenberg, M., Goldbach, R.W., Blissard, G.W., Vlak, J.M., Zuidema, D. 2000Virology2753041Google Scholar
  24. Pearson, M.N., Groten, C., Rohrmann, G.F. 2000J Virol.7461266131Google Scholar
  25. Westenberg, M., Wang, H., IJkel, W.F., Goldbach, R.W., Vlak, J.M., Zuidema, D. 2002J Virol76178184Google Scholar
  26. Lung, O., Westenberg, M., Vlak, J.M., Zuidema, D., Blissard, G.W. 2002J Virol7657295736Google Scholar
  27. Rohrmann G.F. and Karplus P.A., BMC Evol Biol 1, 1, 2001.Google Scholar
  28. Pearson, M.N., Russell, R.L., Rohrmann, G.F. 2001Virology2912231Google Scholar
  29. Lung, O.Y., Cruz-Alvarez,  M., Blissard, G.W. 2003J. Virol.77328339Google Scholar
  30. Kuzio, J., Jaques, R., Faulkner, P. 1989Virology173759763Google Scholar
  31. Faulkner,  P., Kuzio, J., Williams, G.V., Wilson, J.A. 1997J Gen Virol7830913100Google Scholar
  32. Whitford, M., Faulkner, P. 1992J Virol6633243329Google Scholar
  33. Whitford, M., Faulkner, P. 1992J Virol6647634768(Erratum 67, 2427; 1993)Google Scholar
  34. Russell, R.L.Q., Rohrmann, G.F. 1993Virology195532540Google Scholar
  35. Hong, T., Braunagel, S.C., Summers, M.D. 1994Virology204210222Google Scholar
  36. Braunagel, S.C., Elton, D.M., Ma, H., Summers, M.D. 1996Virology21797110Google Scholar
  37. Theilmann, D.A., Chantler, J.K., Stewart, S., Flipsen, H.T.M., Vlak, J.M., Crook, N.E. 1996Virology218148158Google Scholar
  38. Braunagel, S.C., He, H., Ramamurthy, P., Summers, M.D. 1996Virology222100114Google Scholar
  39. Slack,  J.M., Dougherty, E.M., Lawrence, S.D. 2001J Gen Virol8222792287Google Scholar
  40. Kikhno,  I., Gutierrez, S., Croizier, L., Croizier, G., Ferber, M. L. 2002J Gen Virol8330133022Google Scholar
  41. Ayres, M.D., Howard, S.C., Kuzio, J., Lopez-ferber, M., Possee, R.D. 1994Virology202586605Google Scholar
  42. Pijlman, G.P., Pruijssers, A.J.P., Vlak, J.M. 2003J Gen Virol8420412049Google Scholar
  43. Gutiérrez,  S., Kikhno, I., Ferber, M.L. 2004J Gen Virol85331341Google Scholar
  44. Summers M.D., and Smith G.E., Texas Agric Exp Stn Bull, 1987.Google Scholar
  45. Lu, S., Yang, F., Liu, Z., Qi, Y. 2001Chinese J. BiochemMol. Biol17743750(in Chinese; English Abstract)Google Scholar
  46. Hughes, P.R., Wood, H.A. 1981J Invertebr Pathol37154159Google Scholar
  47. Finney, D., Probit Analysis, 3rd ed., Cambridge University Press, London, 1971.Google Scholar
  48. Wolfersberger, M.G., Luthy, P., Maurer, A., Parenti, P., Sacchi, F.V., Giordana, B., Hanozet, G.M. 1987CompBiochem Physiol86301308Google Scholar
  49. Sambrook,  J., Fritsch, E.F., Maniatis, T. 1989Molecular Cloning: A Laboratory Manualsecond ed. Cold Spring Harbor Laborotory PressCold Spring Harbor, NYGoogle Scholar
  50. Derksen, A.C.G., Granados, R.R. 1988Virology167242250Google Scholar
  51. Uchima, K., Harvey, J.P., Omi, E.M., Tanada, Y. 1988Insect Biochemistry18645650Google Scholar
  52. Rossmann, M.G. 1994Prot Sci317121725Google Scholar
  53. Wickhanm, T.J., Granados, R.R., Wood, H.A, Hammer, D.A., Shuler, M.L. 1990Biophys J5815011516Google Scholar
  54. Tardieu,  M., Epstein, R.L., Weiner, H.L. 1982Int Rev Cytol802761Google Scholar
  55. Hollenberg M.D., and Cuatrecasa P. in O’Brien R.D. (ed.). The Receptors: a Comprehensive Treatise, vol. 1, Plenum Press, New York. 1979, pp. 193–214.Google Scholar
  56. Haas-Stapleton, E.J., Washburn, J.O., Volkman, L.E. 2004J Virol7867866791Google Scholar

Copyright information

© Springer Science+Business Media, Inc. 2005

Authors and Affiliations

  • Wenke Zhou
    • 1
  • Lunguang Yao
    • 1
  • Hua Xu
    • 1
  • Feng Yan
    • 1
  • Yipeng Qi
    • 1
    Email author
  1. 1.Ministry of Education Key Laboratory of Virology, College of Life SciencesWuhan UniversityWuhanP. R. China

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