Virus Genes

, Volume 30, Issue 1, pp 113–119

Completion of the Sequence of a Cetacean Morbillivirus and Comparative Analysis of the Complete Genome Sequences of Four Morbilliviruses

Article

Abstract

The gene encoding the large (L) protein and the genome termini of the dolphin strain of cetacean morbillivirus (CeMV) were sequenced. The CeMV genome is 15702 nucleotides long and has been compared with other available morbillivirus genome sequences in regards to the “rule of six” and the “phase” of any particular nucleotide, defined as its position within a given hexamer, which here is defined as a group of six nucleotides starting from the 3′ end of the genomic RNA. With exception of the position of the start of the F gene, the phase of the transcription start sites of each gene is strictly conserved between the morbilliviruses, but each gene is in a different phase. The lengths of gene transcripts differ between viruses by multiples of six nucleotides with exception of the M and F transcripts. The differences between the various morbilliviruses result from deletions or insertions of multiples of six nucleotides in the 3′ and 5′ UTRs of the different viral genes. The four bases were distributed non-randomly over the six positions in the hexamer boxes. However, the distribution patterns of each of the four bases indicated that multiples of three were more prevalent than those of six nucleotides. This reflected the positions of nucleotides in codons and codon usage in the reading frames. The L protein of CeMV was found to be 2183 amino acids in length and similar to that of MV and RPV. The CeMV L protein sequence was found to be equidistant beween those of the CDV/PDV and MV/RPV subgroups of the morbilliviruses. This concurs with the analyses carried out on the other structural proteins.

Keywords

cetacean morbillivirus dolphin morbillivirus paramyxovirus phasing rule of six sequence periodicity 

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References

  1. Appel, M.J.G., Gibbs, E.P.J., Martin, S.J., ter Meulen, V., Rima, B.K., Stephenson, J.R., Taylor, W.P. 1981Kurstag, E.Kurstak, C. eds. Comparative Diagnosis of Viral DiseasesAcademic PressNew York235300vol. IVGoogle Scholar
  2. Cosby, S.L., McQuaid, S., Duffy, N., Lyons, C., Rima, B.K., Allan, G.M., McCullough, S.J., Kennedy, S., Smyth, J.A., McNeilly, F., Craig, C., Õrvell, C. 1988Nature336115116Google Scholar
  3. Mahy, B.W.J., Barrett, T., Evans, S., Anderson, E.C., Bostock, C.J. 1988Nature336115Google Scholar
  4. Kennedy, S., Smyth, J.A., Cush, P.F., McCullough, S.J., Allan, G.M., McQuaid, S. 1988Nature33621Google Scholar
  5. Domingo, M., Ferrer, L., Pumarola, M., Marco, A., Plana, J., Kennedy, S., McAliskey, M., Rima, B.K. 1990Nature34821Google Scholar
  6. Bolt, G., Blixenkrone-Møller, M., Gottschalck, E., Wishaupt, R.G.A., Welsh, M.J., Earle, J.A.P., Rima, B.K. 1994Vir Res34291304Google Scholar
  7. Griffin, D.E., Bellini, W.J. 1996Fields, B.N. eds. VirologyLippincott-Raven PublishersNew York12671312Google Scholar
  8. Bellini, W.J., Englund, G., Rozenblatt, S., Arnheiter, H., Richardson, C.D. 1985J Virol53908919Google Scholar
  9. Cattaneo, R., Kaelin, K, Baczko, K., Billeter, M.A. 1989Cell56759764Google Scholar
  10. Radecke, F., Billeter, M.A. 1995Cur Top Microbiol Immunol191181192Google Scholar
  11. Baron, M.D., Kamata, Y., Barras, V., Goatley, L., Barrett, T. 1996J. Gen Virol7730413046Google Scholar
  12. McIlhatton, M.A., Curran, M.D., Rima, B.K. 1997J Gen Virol78571578Google Scholar
  13. Curran, M.D., Clarke, D.K., Rima, B.K. 1991J Gen Virol72443447Google Scholar
  14. Curran, M.D., O’Loan, D., Kennedy, S., Rima, B.K. 1992J Gen Virol7311891194Google Scholar
  15. Blixenkrone-Møller, M., Bolt, G., Gottschalck, E., Kenter, M. 1994J Gen Virol7528292834Google Scholar
  16. Blixenkrone-Møller, M., Bolt, G., Jensen, T.D., Harder, T., Svansson, V. 1996Vir Res404755Google Scholar
  17. Bolt, G., Alexandersen, S., Blixenkrone-Møller, M. 1995J Gen Virol7630513058Google Scholar
  18. Tapparel, C., Maurice, D., Roux, L. 1998J Virol7231173128Google Scholar
  19. Calain, P., Roux, L. 1993J Virol6748224830Google Scholar
  20. Kolakofsky, D., Pelet, T., Garcin, D., Hausmann, S., Curran, J., Roux, L. 1998J Virol72891899Google Scholar
  21. Rima, B.K., McFerran, N.V. 1997J Gen Virol7828592870Google Scholar
  22. Rima, B.K., Earle, J.A.P., Baczko, K., ter Meulen, V., Liebert, U.G., Carstens, S.C., Carabaña, J., Caballero, M., Celma, M.L., Fernandez-Muñoz, R. 1997J Gen Virol7897106Google Scholar

Copyright information

© Springer Science+Business Media, Inc. 2005

Authors and Affiliations

  • B. K. Rima
    • 1
  • A. M. J. Collin
    • 1
  • J. A. P. Earle
    • 1
  1. 1.School of Biology and BiochemistryThe Queen’s University of BelfastBelfastN.Ireland

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