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Virus Genes

, Volume 30, Issue 1, pp 75–83 | Cite as

Identification of a Naturally Occurring Recombinant Isolate of Sugarcane Mosaic Virus Causing Maize Dwarf Mosaic Disease

  • Yongwang Zhong
  • Anyuan Guo
  • Chunbo Li
  • Binquan Zhuang
  • Ming Lai
  • Chunhong Wei
  • Jingchu Luo
  • Yi liEmail author
Article

Abstract

The complete nucleotide sequence of a potyvirus causing severe maize dwarf mosaic disease in Shaanxi province, northwestern China was determined (GenBank accession No. AY569692). The full genome is 9596 nucleotides in length excluding the 3 ′-terminal poly (A) sequence. It contains a large open reading frame (ORF) flanked by a 149 nt 5′-untranslated region (UTR) and a 255 nt 3′-UTR. The putative polyprotein encoded by this large ORF comprises of 3063 amino acid residues. Sequence comparisons and phylogenetic analyses showed that this potyvirus is an isolate of Sugarcane mosaic virus (SCMV). The entire sequences shared identities of 89.6–97.6 % and 79.3–93.3% with 9 sequenced SCMV isolates at the nucleotide and deduced amino acid levels, respectively. But it showed much lower identities with Maize dwarf mosaic virus (MDMV), Sorghum mosaic virus (SrMV) and Johnsongrass mosaic virus (JGMV) isolates. The putative coat protein sequence is identical to that of a Chinese maize isolate SCMV-HZ. However, partition comparisons and phylogenetic profile analyses of the viral nucleotide sequences indicated that it is a recombinant isolate of SCMV. The recombination sites are located within the 6K1 and CI coding regions.

Keywords

maize dwarf mosaic disease phylogenetic analysis potyvirus Sugarcane mosaic virus Virus recombination 

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Copyright information

© Springer Science+Business Media, Inc. 2005

Authors and Affiliations

  • Yongwang Zhong
    • 1
  • Anyuan Guo
    • 2
  • Chunbo Li
    • 1
  • Binquan Zhuang
    • 1
  • Ming Lai
    • 1
  • Chunhong Wei
    • 1
  • Jingchu Luo
    • 2
  • Yi li
    • 1
    Email author
  1. 1.Peking-Yale Joint Center for Plant Molecular Genetics and Agro-biotechnology, The National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life SciencesPeking UniversityBeijingChina
  2. 2.Centre of BioInformatics, College of Life SciencesPeking UniversityBeijingChina

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