Virus Genes

, Volume 30, Issue 1, pp 23–29 | Cite as

Functional Variability of Rev Response Element in HIV-1 Primary Isolates

  • Angsana Phuphuakrat
  • Prasert Auewarakul


We have previously studied sequence heterogeneity of HIV-1 Rev response element (RRE), and showed uneven variations in different stem–loops of both primary sequence and secondary structure. Here we studied the functional variation of RRE clones from a set of 10 primary isolates, and demonstrated a variation in the function of these RRE clones on the expression of Gag proteins from a truncated HIV-1 genome. The difference in Gag level was, in part, if not exclusively, resulted from the differential efficiency of RNA transport and enhancing of translation. These data suggested that variation of HIV-1 RRE may play a role in regulation of viral replication rate in HIV-1 primary isolates.


HIV-1 RRE variability 


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Copyright information

© Springer Science+Business Media, Inc. 2005

Authors and Affiliations

  • Angsana Phuphuakrat
    • 1
  • Prasert Auewarakul
    • 1
  1. 1.Department of Microbiology, Faculty of Medicine Siriraj HospitalMahidol UniversityBangkokThailand

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