Advertisement

Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

CD14-dependent Modulation of Transcriptional Activities of Endogenous Retroviruses in the Lung after Injury

Abstract

Lipopolysaccharide (LPS) plays a central role in the pathogenesis of distant organs after burn. Recent studies demonstrated the regulation of mouse endogenous retroviruses (MuERVs) in several organs after burn. In this study, the role of CD14, a LPS receptor, in burn-mediated regulation of MuERV expression in the lung was investigated. CD14 knockout (KO) and wild type (WT) mice were subjected to burn followed by RT-PCR analysis of alterations in the MuERV expression in the lung 1 day after injury. Even without injury, CD14 KO mice had a unique profile of MuERV expression compared to WT. Three bands (Lung-1, Lung-2, and Lung-3) in CD14 KO were downregulated after injury. Lung-2 and Lung-3 transcripts were almost identical to 2 previously described defective env transcripts of MuERVs, respectively. The Lung-1-1 transcript was a double spliced message generated by the env and a set of novel splicing signals, whereas the Lung-1-2 transcript was a defective env transcript. Only the Lung-1-1 transcript had a significant ORF capable of encoding a gag-pol fusion polypeptide. Putative proviral sequences of Lung-1-1 and Lung-1-2 transcripts were mapped to chromosomes 4 and 11, respectively. The results from this study suggest that the absence of CD14 expression in CD14 KO mice contributes to the transcriptional regulation of MuERVs in the lung after injury.

This is a preview of subscription content, log in to check access.

References

  1. A.F. Smit (1999) Curr Opin Genet Dev 9 657–663 Occurrence Handle1:CAS:528:DC%2BD3cXhslOisA%3D%3D Occurrence Handle10607616 Occurrence Handle10.1016/S0959-437X(99)00031-3

  2. E.S. Lander L.M. Linton B. Birren C. Nusbaum M.C. Zody J. Baldwin K. Devon K. Dewar M. Doyle W. FitzHugh R. Funke D. Gage K. Harris A. Heaford J. Howland L. Kann J. Lehoczky R. LeVine P. McEwan K. McKernan J. Meldrim J.P. Mesirov C. Miranda W. Morris J. Naylor C. Raymond M. Rosetti R. Santos A. Sheridan C. Sougnez N. Stange-Thomann N. Stojanovic A. Subramanian D. Wyman J. Rogers D. Bentley S. Beck R. Ainscough J. Sulston J. Burton C. Clee N. Carter A. Coulson R. Deadman P. Deloukas A. Dunham I. Dunham R. Durbin L. French D. Grafham S. Gregory T. Hubbard S. Humphray A. Hunt M. Jones C. Lloyd A. McMurray L. Matthews S. Mercer S. Milne J.C. Mullikin A. Mungall R. Plumb M. Ross R. Shownkeen S. Sims R.H. Waterston R.K. Wilson L.W. Hillier J.D. McPherson M.A. Marra E.R. Mardis L.A. Fulton A.T. Chinwalla K.H. Pepin W.R. Gish S.L. Chissoe M.C. Wendl K.D. Delehaunty T.L. Miner A. Delehaunty J.B. Kramer L.L. Cook R.S. Fulton D.L. Johnson P.J. Minx S.W. Clifton T. Hawkins E. Branscomb P. Predki P. Richardson S. Wenning T. Slezak N. Doggett J.F. Cheng A. Olsen S. Lucas C. Elkin E. Uberbacher M Frazier et al. (2001) Nature 409 860–921 Occurrence Handle1:CAS:528:DC%2BD3MXhsFCjtLc%3D Occurrence Handle11237011 Occurrence Handle10.1038/35057062

  3. J.C. Venter M.D. Adams E.W. Myers P.W. Li R.J. Mural G.G. Sutton H.O. Smith M. Yandell C.A. Evans R.A. Holt J.D. Gocayne P. Amanatides R.M. Ballew D.H. Huson J.R. Wortman Q. Zhang C.D. Kodira X.H. Zheng L. Chen M. Skupski G. Subramanian P.D. Thomas J. Zhang G.L. Gabor Miklos C. Nelson S. Broder A.G. Clark J. Nadeau V.A. McKusick N. Zinder A.J. Levine R.J. Roberts M. Simon C. Slayman M. Hunkapiller R. Bolanos A. Delcher I. Dew D. Fasulo M. Flanigan L. Florea A. Halpern S. Hannenhalli S. Kravitz S. Levy C. Mobarry K. Reinert K. Remington J. Abu-Threideh E. Beasley K. Biddick V. Bonazzi R. Brandon M. Cargill I. Chandramouliswaran R. Charlab K. Chaturvedi Z. Deng V. Di Francesco P. Dufnn K. Eilbeck C. Evangelista A.E. Gabrielian W. Gan W. Ge F. Gong Z. Gu P. Guan T.J. Heiman M.E. Higgins R.R. Ji Z. Ke K.A. Ketchum Z. Lai Y. Lei Z. Li J. Li Y. Liang X. Lin F. Lu G.V. Merkulov N. Milshina H.M. Moore A.K. Naik V.A. Narayan B. Neelam D. Nusskern D.B. Rusch S. Salzberg W. Shao B. Shue J. Sun Z. Wang A. Wang X. Wang J. Wang M. Wei R. Wides C. Xiao C. Yan et al. (2001) Science 291 1304–1351 Occurrence Handle1:CAS:528:DC%2BD3MXhtlSgsbo%3D Occurrence Handle11181995 Occurrence Handle10.1126/science.1058040

  4. A.M. Krieg M.F. Gourley A. Perl (1992) Faseb. J 6 2537–2544 Occurrence Handle1:CAS:528:DyaK38XktVGmu7Y%3D Occurrence Handle1592206 Occurrence Handle10.1096/fasebj.6.8.1592206

  5. A. Ranki P. Kurki S. Riepponen E. Stephansson (1992) Arthritis Rheum 35 1483–1491 Occurrence Handle1:STN:280:DyaK3s7ht1Wnug%3D%3D Occurrence Handle1472125 Occurrence Handle10.1002/art.1780351212

  6. J.E. Deas L.G. Liu J.J. Thompson D.M. Sander S.S. Soble R.F. Garry W.R. Gallaher (1998) Clin Diagn Lab Immunol 5 181–185 Occurrence Handle1:CAS:528:DyaK1cXitlCitbY%3D Occurrence Handle9521140 Occurrence Handle121355

  7. H.B. Rasmussen H. Perron J. Clausen (1993) Acta Neurol Scand 88 190–198 Occurrence Handle1:STN:280:DyaK2c%2FnvVOjug%3D%3D Occurrence Handle8256555 Occurrence Handle10.1111/j.1600-0404.1993.tb04215.x

  8. B. Conrad R.N. Weissmahr J. Boni R. Arcari J. Schupbach B. Mach (1997) Cell 90 303–313 Occurrence Handle1:CAS:528:DyaK2sXltVCjt7g%3D Occurrence Handle9244304 Occurrence Handle10.1016/S0092-8674(00)80338-4

  9. S.S. Kalter R.J. Helmke M. Panigel R.L. Heberling P.J. Felsburg L.R. Axelrod (1973) Science 179 1332–1333 Occurrence Handle1:STN:280:DyaE3s%2FpslGltw%3D%3D Occurrence Handle4631426 Occurrence Handle10.1126/science.179.4080.1332

  10. S. Maeda R.C. Mellors J.W. Mellors L.B. Jerabek I.A. Zervoudakis (1983) Am J Pathol 112 347–356 Occurrence Handle1:STN:280:DyaL3szgs1Sguw%3D%3D Occurrence Handle6311020 Occurrence Handle1916391

  11. T.W. Lyden P.M. Johnson J.M. Mwenda N.S. Rote (1994) Biol Reprod 51 152–157 Occurrence Handle1:STN:280:DyaK2M%2Fgt1Cjug%3D%3D Occurrence Handle7522592 Occurrence Handle10.1095/biolreprod51.1.152

  12. P.J. Venables S.M. Brookes D. Griffiths R.A. Weiss M.T. Boyd (1995) Virology 211 589–592 Occurrence Handle1:CAS:528:DyaK2MXnsFGjsL4%3D Occurrence Handle7645262 Occurrence Handle10.1006/viro.1995.1442

  13. K. Cho D. Greenhalgh (2003) Virus Res 93 189–198 Occurrence Handle1:CAS:528:DC%2BD3sXktFWmtrk%3D Occurrence Handle12782367 Occurrence Handle10.1016/S0168-1702(03)00097-2

  14. K. Cho L.K. Adamson D.G. Greenhalgh (2002) J Surg Res 104 53–62 Occurrence Handle1:CAS:528:DC%2BD38XjtVKksbk%3D Occurrence Handle11971678 Occurrence Handle10.1006/jsre.2002.6410

  15. M.G. Schwacha (2003) Burns 29 1–14 Occurrence Handle12543039 Occurrence Handle10.1016/S0305-4179(02)00187-0

  16. J.G. Williams P. Bankey J.P. Minei K. McIntyre T. Turbeville (1994) J Burn Care Rehabil 15 493–498 Occurrence Handle1:STN:280:DyaK2M7lsFCltQ%3D%3D Occurrence Handle7852452 Occurrence Handle10.1097/00004630-199411000-00006

  17. Kelly J.L., O’Sullivan C., O’Riordain M., O’Riordain D., Lyons A., Doherty J., Mannick J.A., and Rodrick M.L., Ann Surg 225, 530–541; discussion 541–543, 1997.

  18. P.Y. Xia J. Zheng H. Zhou W.D. Pan X.J. Qin G.X. Xiao (2002) World J Gastroenterol 8 546–550 Occurrence Handle1:CAS:528:DC%2BD38XlsF2ks7o%3D Occurrence Handle12046089 Occurrence Handle4656440 Occurrence Handle10.3748/wjg.v8.i3.546

  19. K. Cho R.I. Zipkin L.K. Adamson A.L. McMurtry S.M. Griffey D.G. Greenhalgh (2000) Shock 14 182–186 Occurrence Handle1:STN:280:DC%2BD3M%2FovFyltg%3D%3D Occurrence Handle10947164 Occurrence Handle10.1097/00024382-200014020-00018

  20. B.C. Cho J.D. Shaughnessy SuffixJr. D.A. Largaespada H.G. Bedigian A.M. Buchberg N.A. Jenkins N.G. Copeland (1995) J Virol 69 7138–7146 Occurrence Handle1:CAS:528:DyaK2MXoslWrsro%3D Occurrence Handle7474134 Occurrence Handle189634

  21. S.K. Chattopadhyay H.C. Morse III M. Makino S.K. Ruscetti J.W. Hartley (1989) Proc Natl Acad Sci USA 86 3862–3866 Occurrence Handle1:CAS:528:DyaL1MXktlCgsLk%3D Occurrence Handle2542949 Occurrence Handle10.1073/pnas.86.10.3862

  22. D.C. Aziz Z. Hama P. Jolicoeur (1989) Nature 338 505–508 Occurrence Handle1:CAS:528:DyaK3cXhsFan Occurrence Handle2538760 Occurrence Handle10.1038/338505a0

  23. K. Tomonaga J.M. Coffin (1999) J Virol 73 4327–4340 Occurrence Handle1:CAS:528:DyaK1MXis1ers7Y%3D Occurrence Handle10196331 Occurrence Handle104214

Download references

Author information

Correspondence to David G. Greenhalgh.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Cho, K., Pham, T.N. & Greenhalgh, D.G. CD14-dependent Modulation of Transcriptional Activities of Endogenous Retroviruses in the Lung after Injury. Virus Genes 30, 5–12 (2005). https://doi.org/10.1007/s11262-004-4576-y

Download citation

Keywords

  • Burn
  • CD14
  • Endogenous retroviruses
  • Lung
  • Splicing