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Veterinary Research Communications

, Volume 43, Issue 2, pp 131–142 | Cite as

Intestinal gene expressions in broiler chickens infected with Escherichia coli and dietary supplemented with probiotic, acidifier and synbiotic

  • Ahmed I. Ateya
  • Nagah ArafatEmail author
  • Rasha M. Saleh
  • Hanaa M. Ghanem
  • Doaa Naguib
  • Hend A. Radwan
  • Y. Y. Elseady
Original Article
  • 169 Downloads

Abstract

In this study, we investigated the effects of probiotic, acidifier and synbiotic supplementation on growth performance, mortality rate, intestinal gene expressions, fecal shedding, and organs colonization induced by Escherichia coli in broiler chickens. Six experimental groups were included; negative control group (NC), positive control group (PC), probiotic group (PR), acidifier group (AC), synbiotic group (SY) and colistin sulfate group (CS). Chickens in groups NC and PC were fed a basal diet, while chickens in groups PR, AC, SY, and CS were fed a basal diet containing probiotic, acidifier, synbiotic and colistin sulfate, respectively from the 1st day to the 28th day of age. At 7 days of age, all groups (not NC) were orally challenged with 0.5 ml (1.0 × 109 CFU/ml) E. coli O78. The dietary supplementation of acidifier and synbiotic were sufficient to quell the devastating effects of E. coli infection in broilers. Growth performances represented by body weight gain, feed intake and feed conversion ratio were significantly improved as well as, mortalities were prevented whilst the ileal pro-inflammatory gene expressions (IL-6, IL-8, IL-13, TLR-4, IFN-γ, LITAF, AvBD-2, and AvBD-9) were significantly downregulated and the anti-inflammatory cytokine (IL-10) was significantly increased. In addition, E. coli fecal shedding and organs colonization was significantly diminished. It was concluded that the addition of both acidifier and synbiotic to the diet of broilers infected with E. coli could modulate the intestinal inflammatory responses induced by E. coli infection and minimized the inflammation-induced damage which resulted in improvement in growth performance, prevention of mortalities and reduction of E. coli environmental contamination.

Keywords

Escherichia coli Gene expressions Broilers Acidifiers Synbiotic 

Notes

Acknowledgements

Authors wish to thank departments of Poultry diseases, Animal Husbandry and Wealth Development, Physiology and Hygiene and Zoonoses for their help, encouragement, and support during the course of this study.

Funding

This work did not get any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Department of Animal Husbandry and Wealth Development, Faculty of Veterinary MedicineMansoura UniversityMansouraEgypt
  2. 2.Department of Poultry Diseases, Faculty of Veterinary MedicineMansoura UniversityMansouraEgypt
  3. 3.Department of Physiology, Faculty of Veterinary MedicineMansoura UniversityMansouraEgypt
  4. 4.Department of Hygiene and Zoonoses, Faculty of Veterinary MedicineMansoura UniversityMansouraEgypt

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