Abstract
Dendritic cells (DC) are important antigen-presenting cells and are among the least characterized immune cells in the chicken. In order to obtain chicken DC, current protocols require isolation of bone marrow myeloid progenitor cells and induction of DC differentiation with supplemental cytokines or negative selection of splenic cell preparations. Chicken peritoneal exudate cells (PEC) have traditionally been a source of various immune cells for ex vivo studies, primarily to investigate heterophils and macrophages. In this study, we observe the presence of CD205+ PEC populations, a marker of DC, as an additional resource to isolate and study chicken primary DCs. A panel of monoclonal antibodies was developed against the chicken CD205 DC marker and used to isolate CD205+ DC from the PEC population using magnetic bead cell sorting. This study reports the development of new anti-CD205 monoclonal antibodies as a reagent for chicken DC research, as well as PEC as a potential source of CD205+ DC for ex vivo studies in the chicken.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bonifaz LC, Bonnyay DP, Charalambous A, Darguste DI, Fujii SI, Soares H, Brimnes MK, Moltedo B, Moran TM, Steinman RM (2004) In vivo targeting of antigens to maturing dendritic cells via the DEC-205 receptor improves T cell vaccination. J Exp Med 199:815–824. https://doi.org/10.1084/jem.20032220
Chen CH, Abi-Ghanem D, Njongmeta L, Bray J, Mwangi W, Waghela SD, McReynolds JL, Ing NH, Berghman LR (2010) Production and characterization of agonistic monoclonal antibodies against chicken CD40. Dev Comp Immunol 34:1139–1143. https://doi.org/10.1016/j.dci.2010.06.014
Dudziak D, Kamphorst AO, Heidkamp GF, Buchholz VR, Trumpfheller C, Yamazaki S, Cheong C, Liu K, Lee HW, Park CG, Steinman RM, Nussenzweig MC (2007) Differential antigen processing by dendritic cell subsets in vivo. Science 315:107–111. https://doi.org/10.1126/science.1136080
Fukaya T, Murakami R, Takagi H, Sato K, Sato Y, Otsuka H, Ohno M, Hijikata A, Ohara O, Hikida M, Malissen B, Sato K (2012) Conditional ablation of CD205+ conventional dendritic cells impacts the regulation of T-cell immunity and homeostasis in vivo. Proc Natl Acad Sci U S A 109:11288–11293. https://doi.org/10.1073/pnas.1202208109
Heath WR, Carbone FR (2009) Dendritic cell subsets in primary and secondary T cell responses at body surfaces. Nat Immunol 10:1237–1244. https://doi.org/10.1038/ni.1822
Hurwitz AA, Watkins SK (2012) Immune suppression in the tumor microenvironment: a role for dendritic cell-mediated tolerization of T cells. Cancer Immunol Immunother 61:289–293. https://doi.org/10.1007/s00262-011-1181-5
Jauregui-Zuniga D et al (2017) Targeting antigens to Dec-205 on dendritic cells induces a higher immune response in chickens: hemagglutinin of avian influenza virus example. Res Vet Sci 111:55–62. https://doi.org/10.1016/j.rvsc.2016.12.002
Jiang W, Swiggard WJ, Heufler C, Peng M, Mirza A, Steinman RM, Nussenzweig MC (1995) The receptor DEC-205 expressed by dendritic cells and thymic epithelial cells is involved in antigen processing. Nature 375:151–155. https://doi.org/10.1038/375151a0
Paul WE (2013) Fundamental immunology, 7th edn. Wolters Kluwer Health/Lippincott Williams & Wilkins, Philadelphia
Quere P, Pierre J, Hoang MD, Esnault E, Domenech J, Sibille P, Dimier-Poisson I (2013) Presence of dendritic cells in chicken spleen cell preparations and their functional interaction with the parasite toxoplasma gondii. Vet Immunol Immunopathol 153:57–69. https://doi.org/10.1016/j.vetimm.2013.02.007
Sabet T, Wen-Cheng H, Stanisz M, El-Domeiri A, Van Alten P (1977) A simple method for obtaining peritoneal macrophages from chickens. J Immunol Methods 14:103–110
Shrimpton RE, Butler M, Morel AS, Eren E, Hue SS, Ritter MA (2009) CD205 (DEC-205): a recognition receptor for apoptotic and necrotic self. Mol Immunol 46:1229–1239. https://doi.org/10.1016/j.molimm.2008.11.016
Staines K, Young JR, Butter C (2013) Expression of chicken DEC205 reflects the unique structure and function of the avian immune system. PLoS One 8:e51799. https://doi.org/10.1371/journal.pone.0051799
Staines K, Hunt LG, Young JR, Butter C (2014) Evolution of an expanded mannose receptor gene family. PLoS One 9:e110330. https://doi.org/10.1371/journal.pone.0110330
Wu Z, Rothwell L, Young JR, Kaufman J, Butter C, Kaiser P (2010) Generation and characterization of chicken bone marrow-derived dendritic cells. Immunology 129:133–145. https://doi.org/10.1111/j.1365-2567.2009.03129.x
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
The authors declare that they have no conflict of interest.
Ethical approval
All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. All procedures performed in studies involving animals were in accordance with the ethical standards of the institution or practice at which the studies were conducted.
Additional information
Publisher’s note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Vuong, C.N., Chou, WK. & Berghman, L.R. CD205-positive, Sepharose-induced peritoneal exudate cells: a new resource for DC research in the chicken. Vet Res Commun 43, 115–122 (2019). https://doi.org/10.1007/s11259-019-09751-1
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11259-019-09751-1