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Molecular characterization and xenogenic application of wharton’s jelly derived caprine mesenchymal stem cells

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Abstract

Aim of the present study was in vitro expansion and characterization of caprine wharton’s jelly derived mesenchymal stem cells (cWJ-MSCs) to investigate their tissue healing potential in xenogenic animal model. Plastic adherent fibroblastoid cell populations with distinctive homogeneous morphology were isolated from caprine wharton’s jelly explants. These wharton’s jelly derived cells were found positive for the surface markers CD-73, STRO-1 and CD-105, whereas they were negative for hematopoetic stem cell marker CD-34. In vitro cultured cWJ-MSCs also showed differentiation properties into osteogenic, adipogenic and chondrogenic lineages as demonstrated by von Kossa, Oil Red- O and Alcian blue staining respectively, which was further confirmed and quantified by flow cytometric analysis. Furthermore, these well characterized cWJ-MSCs were evaluated for the wound-healing potential in full-thickness skin wounds in rabbit model for 28 days. Caprine WJ- MSCs treated skin wounds showed significantly (P < 0.05) higher percentage of wound contraction especially at the 21st day post transplantation when compared to PBS treated control group animals. Further, we observed better healing potential of cWJ-MSCs in terms of histo-morphological evaluation, epithelialisation and collagenization with matured vascularization stage by day 28 as compared to control. In conclusion, cWJ- MSCs provide an alternative inexhaustible source of mesenchymal stem cells and also unravel new perspectives pertaining to the therapeutic use of these cells in different species.

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Acknowledgement

Authors are thankful to Indian Council of Agricultural Research (ICAR) for providing necessary financial assistance.

Conflict of interest

None of the authors have any conflict of interest to declare.

Authors contribution

Dr. Pratheesh and Dr. Nitin Gade isolated and maintained mesenchymal stem cells and executed characterization. Mr. AmarNath and Dr. Pawan K Dubey were associated with protein localization experiments. Dr. Amarpal, Dr. Sivanarayanan and Dr. Madhu contributed in therapeutical experiment. Dr. Bhaskar Sharma provided facility of Fluorescent activated cell sorter. Dr. G Sai Kumar evaluated all the histological data for therapeutic evaluation and Dr. G Taru Sharma designed, planned the execution of present study.

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Authors and Affiliations

  1. Division of Physiology and Climatology, Indian Veterinary Research Institute, Izatnagar, Bareilly, U.P., India

    M. D. Pratheesh, Nitin E. Gade, Pawan K. Dubey, Amar Nath & G. Taru Sharma

  2. Division of Surgery, Indian Veterinary Research Institute, Izatnagar, Bareilly, U.P., India

    T. B. Sivanarayanan, D. N. Madhu &  Amarpal

  3. Division of Pathology, Indian Veterinary Research Institute, Izatnagar, Bareilly, U.P., India

    G. Saikumar

  4. Division of Biochemistry, Indian Veterinary Research Institute, Izatnagar, Bareilly, U.P., India

    Bhaskar Sharma

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  1. M. D. Pratheesh
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  2. Nitin E. Gade
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  3. Pawan K. Dubey
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  5. T. B. Sivanarayanan
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  6. D. N. Madhu
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  7. Bhaskar Sharma
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  8. Amarpal
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  9. G. Saikumar
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  10. G. Taru Sharma
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Corresponding author

Correspondence to G. Taru Sharma.

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Pratheesh, M.D., Gade, N.E., Dubey, P.K. et al. Molecular characterization and xenogenic application of wharton’s jelly derived caprine mesenchymal stem cells. Vet Res Commun 38, 139–148 (2014). https://doi.org/10.1007/s11259-014-9597-y

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  • DOI: https://doi.org/10.1007/s11259-014-9597-y

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