Veterinary Research Communications

, Volume 35, Issue 4, pp 237–244 | Cite as

The curative and antioxidative efficiency of ivermectin and ivermectin + vitamin E-selenium treatment on canine Sarcoptes scabiei infestation

  • Suvendu Kumar BeheraEmail author
  • Umesh Dimri
  • Shanker Kumar Singh
  • Ranjan Kumar Mohanta
Original Article


The objective of the present study was to investigate the curative and antioxidative efficacy of ivermectin and ivermectin + vitamin E-selenium, and the influence of these agents on oxidative stress parameters in canines infested by Sarcoptes scabiei. Twenty two sarcoptic mites infested dogs and nine healthy dogs of 6 months to 2 years of age were divided into three groups. Group I comprised of healthy dogs (n = 9) whereas animals in group II (n = 11) and III (n = 11) were positive for scabies. Group II animals were treated with only 1% ivermectin @ 0.2 mg/kg SC whereas group III were additionally treated with Vitamin E and selenium (tocopherol 50 mg + Se 1.5 mg/ml) @0.5 ml/20 kg IM at weekly intervals for three times. Blood samples were collected on day 0 and 28 post therapy. The values for hemato-biochemical parameters and activities of antioxidant enzymes were significantly decreased (P < 0.05) whereas level of lipid peroxidation was significantly increased in all the infested dogs in comparison to the healthy dogs on day 0 which approached normalcy by day 28 post therapy. The dogs of group III showed better clinical recovery in comparison to group II at the end of therapy. Thus, administration of vitamin E and selenium in addition to standard therapy can alleviate these alterations hastening the clinical recovery of diseased dogs and can be recommended as an adjunct therapy with miticides for canine sarcoptic mange.


Antioxidant Lipid peroxidation Oxidative stress Sarcoptic mange 


  1. Arlian LG, Morgan MS, Rapp CM, Vyszenski-moher DL (1995) Some effects of sarcoptic mange on dogs. J Parasitol 81(5):698–702PubMedCrossRefGoogle Scholar
  2. Bergmeyer HU (1983) UV method of catalase assay. In: Methods of Enzymatic Analysis, Vol 3rd, Weinheim. Deer Field Beach, Florida, pp 273Google Scholar
  3. Camkerten I, Sahin T, Borazan G, Gokcen A, Erel O, Das A (2009) Evaluation of blood oxidant/antioxidant balance in dogs with sarcoptic mange. Vet Parasitol 161:106–109PubMedCrossRefGoogle Scholar
  4. Chandy J, Nambi AP, Jeyaraja K, Gowri B (2000) Clinicopathological and biochemical studies in scabies in dogs. Indian Vet J 77:755–757Google Scholar
  5. Dacie JV, Lewis SM (1968) Practical hematology, 4th edn. J. and A. Churchill, UK, p 37Google Scholar
  6. Dadhich H, Khanna R (2008) Pathological, haemato-biochemical and immunological studies of cutaneous ectoparasitoses in dogs. In: Proceedings of the 15th Congress of FAVA. FAVA-OIE Joint Symposium on Emerging Diseases. Bangkok, Thailand.Google Scholar
  7. De UK, Dey S (2010) Evaluation of organ function and oxidant/antioxidant status in goats with sarcoptic mange. Trop Anim Health Prod 42:1663–1668PubMedCrossRefGoogle Scholar
  8. Dimri U, Ranjan R, Singh SK, Sharma MC, Swarup D, Diwedi P, Sharma AK, Kataria M (2007) Clinico-pathological and hemato-biochemical changes in buffaloes with sarcoptic mange. Indian J Vet Pathol 31:160–162Google Scholar
  9. Dimri U, Ranjan R, Kumar N, Sharma MC, Swarup D, Sharma B, Kataria M (2008a) Changes in oxidative stress indices, zinc and copper concentrations in blood in canine demodicosis. Vet Parasitol 154:98–102PubMedCrossRefGoogle Scholar
  10. Dimri U, Sharma MC, Swarup D, Ranjan R, Kataria M (2008b) Alterations in hepatic lipid peroxides and antioxidant profile in Indian water buffaloes suffering from sarcoptic mange. Res Vet Sci 85:101–105PubMedCrossRefGoogle Scholar
  11. Dimri U, Sharma MC, Yamdagni A, Ranjan R, Zama MMS (2009) Psoroptic mange infestation increases oxidative stress and decreases antioxidant status in sheep. Vet Parasitol 168:318–322PubMedCrossRefGoogle Scholar
  12. Dimri U, Ranjan R, Sharma MC, Varshney VP (2010) Effect of vitamin E and selenium supplementation on oxidative stress indices and cortisol level in blood in water buffaloes during pregnancy and early postpartum period. Trop Anim Health Prod 42:405–410PubMedCrossRefGoogle Scholar
  13. El-Khodery SA, Osman SA, Ishii M, Al-Gaabary MH (2010) Risk factors of infestation by Psoroptes spp. mites in buffalo (Bubalus bubalis) at smallholder farms in the Nile Delta region, Egypt. Trop Anim Health Prod 42:275–281PubMedCrossRefGoogle Scholar
  14. Fang YZ, Yang S, Wu G (2002) Free radicals, antioxidants and nutrition. Nutrition 18:872–879PubMedCrossRefGoogle Scholar
  15. Feldman BF, Zinkl JG, Jain NC (2000) Schalm’s Veterinary Hematology, 5th edn. Lippincott Williams and WilkinsGoogle Scholar
  16. Gupta VK, Sharma SK (2006) Plants as natural antioxidants. Nat Prod Rad 5(4):326–333Google Scholar
  17. Hirudkar US, Desphande PD, Narladkar BW, Vadlamudi VP (1997) Effect of herbal treatment with Himax ointment and Neem oil in sarcoptic mange in sheep. Indian Vet J 74:506Google Scholar
  18. ICAR (2008) Handbook of animal husbandry. Indian Council of Agricultural Research, New DelhiGoogle Scholar
  19. Kanbur M, Atalay O, Ica A, Eraslan G, Cam Y (2008) The curative and antioxidative efficiency of doramectin and doramectin + vitamin AD3E treatment on Psoroptes cuniculi infestation in rabbits. Res Vet Sci 85(2):291–293PubMedCrossRefGoogle Scholar
  20. Kemp DJ, Walton SF, Harumal P, Currie BJ (2002) The scourge of scabies. Biologist 49:19–24PubMedGoogle Scholar
  21. Kovacic P, Cooksy A (2005) Iminium metabolite mechanism for nicotinic toxicity and addiction: oxidative stress and electron transfer. Med Hypotheses 64:104–111PubMedCrossRefGoogle Scholar
  22. Linares MV, Sanchez DJ, Belles M, Albina ML, Gomez M, Domingo JL (2007) Pro-oxidant effects in the brain of rats concurrently exposed to uranium and stress. Toxicology 236:82–91PubMedCrossRefGoogle Scholar
  23. Luqman S, Rizvi SI (2006) Protection of lipid peroxidation and carbonyl formation in protein by capsaicin in human erythrocytes subjects to oxidative stress. Phytother Res 20:303–306PubMedCrossRefGoogle Scholar
  24. Lykkesfeldt J (2002) Increased oxidative damage in vitamin c deficiency is accompanied by induction of ascorbic acid recycling capacity in young but not mature guinea pigs. Free Radic Res 36(5):567–574PubMedCrossRefGoogle Scholar
  25. Menami M, Yoshikawa H (1979) Simplified assay method of superoxide dismutase activity of clinical use. Clin Chim Acta 92:337–342CrossRefGoogle Scholar
  26. Morrow JD (2000) The isoprostanes: Their quantification as an index of oxidant stress status in vivo. Drug Metab Rev 32:377–385PubMedCrossRefGoogle Scholar
  27. Patel JS, Patel RR, Panchasara HH, Brahamaxtri KG (2003) Epizootiology of sarcoptic mange in buffalo calves. Indian Vet J 80:972–974Google Scholar
  28. Placer ZA, Cushman LL, Johnson B (1966) Estimation of product of lipid peroxidation (malonaldehyde) in biochemical system. Anal Biochem 16:359–364PubMedCrossRefGoogle Scholar
  29. Portugal LR, Fernandes LR, Pietra Pedroso VS, Santiago HC, Gazzinelli RT, Alvarez-Leite JI (2008) Influence of low-density lipoprotein receptor on lipid composition, inflammation and parasitism during Toxoplasma gondii infection. Microb Infect 10:276–284CrossRefGoogle Scholar
  30. Prins HK, Loos JA (1969) Biochemical Methods in Red Cell Genetics. Academic Press, Pp-127–129Google Scholar
  31. Saleh MA, Mahran OM, Al-Salahy MB (2011) Circulating oxidative stress status in dromedary camels infested with sarcoptic mange. Vet Res Commun 35:35–45PubMedCrossRefGoogle Scholar
  32. Snedecor GW, Cochran WG (1994) Statistical methods. Iowa State University Press, AmesGoogle Scholar
  33. Soulsby EJL (2006) Helminths, arthropods and protozoa of domestic animals, 7th edn. Bailliere Tindall, LondonGoogle Scholar
  34. SPSS (2002) Statistical Packages for Social Sciences, Version 11.0, SPSS Inc., Illinosis, USAGoogle Scholar
  35. Trouba KJ, Hamadeh HK, Amin RP, Germolec DR (2002) Oxidative stress and its role in skin disease. Antioxid Redox Signal 4:665–673PubMedCrossRefGoogle Scholar
  36. Twomey DF, Birch ES, Schock A (2009) Outbreak of sarcoptic mange in alpacas (Vicugna pacos) and control with repeated subcutaneous ivermectin injections. Vet Parasitol 159:186–191PubMedCrossRefGoogle Scholar
  37. Valko M, Leibfritz D, Moncol J, Cronin MTD, Mazur M, Telser J (2007) Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol 39:44–84PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Suvendu Kumar Behera
    • 1
    Email author
  • Umesh Dimri
    • 1
  • Shanker Kumar Singh
    • 1
  • Ranjan Kumar Mohanta
    • 2
  1. 1.Division of MedicineIndian Veterinary Research InstituteBareillyIndia
  2. 2.Animal Nutrition DivisionIndian Veterinary Research InstituteBareillyIndia

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