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Investigation of selected biochemical indicators of Equine Rhabdomyolysis in Arabian horses: pro-inflammatory cytokines and oxidative stress markers

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Abstract

A total of 30 horses were divided into two groups, one served as a control whereas other was rhabdomyolysis diseased horses. After blood collection, the resulted sera were used for estimation of the activities of creatin kinase (CK), aspartate transaminase (AST), lactate dehydrogenase (LDH), lactic acid, triacylglycerol (TAG), glucose, total protein, albumin, globulin, urea, creatinine, Triiodothyronine (T3), calcium, sodium, potassium, phosphorus, chloride, vitamin E, interleukin-6 (IL-6) and tumor necrosis-α (TNF-α). In addition, whole blood was used for determination of selenium, reduced glutathione (G-SH) and prostaglandin F2-α (PGF2α). The erythrocyte hemolysates were used for the determination of the activities of super oxide dismutase (SOD), catalase (CAT), total antioxidant capacity (TAC), nitric oxide (NO) and malondialdehyde (MDA). The present findings revealed a significant (p ≤ 0.05) increase in the values of CK, AST, LDH, glucose, lactate, TAG, urea, creatinine, phosphorus, MDA, TNF- α, IL6 and PGF2- α in diseased horses when compared with the control. Furthermore, the values of calcium, SOD, CAT, TAC, NO and GSH in diseased horses were significantly (p ≤ 0.05) lower than the control. The other examined parameters were not statistically significant. In conclusion, the examined pro-inflammatory cytokines were useful biomarkers for the diagnosis of Equine rhabdomyolysis (ER) in Arabian horses beside the old examined biomarkers. In the future, efforts should be made to confirm this in other breed. If this could be achieved, it would open up new perspectives in research fields dealing with ER.

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References

  • Aebi H (1984) Catalase in vitro. Meth Enzymol 105:121–126

    Article  CAS  PubMed  Google Scholar 

  • Art T, Kirschvink N, Smith N, Lekeux P (1999) Indices of oxidative stress in blood and pulmonary epithelium lining fluid in horses suffering from recurrent airway obstruction. Equine Vet J 31:397–401

    Article  CAS  PubMed  Google Scholar 

  • Assmann H, Bielenstein H, Hobs H (1933) Beobachtungen und untersuchungen bei der haffkrankheit. Dtsch Med Wochenschr 59:122–6

    Article  Google Scholar 

  • Balogh N, Gaal T, Ribiczeyne S, Petri A (2001) Biochemical and antioxidant changes in plasma and erythrocytes of pentathlon horses before and after exercise. Vet Clin Pathol 30:214–218

    Article  PubMed  Google Scholar 

  • Balon T, Nadler L (1994) Nitric oxide release is present from incubated skeletal muscle preparations. J Appl Physiol 77:2519–2521

    CAS  PubMed  Google Scholar 

  • Brandt K, Hinrichs U, Glitz F, Landes E, Schulze C, Deegen E, Pohlenz J, Coenen M (1997) Atypische myoglobinurie der weidepferde. Pferdeheilkunde 13:27–34

    Google Scholar 

  • Carlström B (1931) Über die Ätiologie und Pathogenese der Kreuzlähmung des Pferdes (Hämoglobinämia paralytica) Skan. Arch Physiol 61:161–168

    Google Scholar 

  • Carlström B (1932) Über die Ätiologie und Pathogenese der Kreuzlähmung des Pferdes (Hämoglobinämia paralytica) Skan. Arch Physiol 63:164–169

    Google Scholar 

  • Clarkson PM (2002) Exertional rhabdomyolysis: myths and madness. Am J Sports Med 4:155–156

    Google Scholar 

  • Clarkson M, Thompson S (2000) Antioxidants: what role do they play in physical activity and health? Am J Clin Nutr 72:637S–646

    CAS  PubMed  Google Scholar 

  • Coles H (1974) Veterinary clinical pathology. Saunders, Philadelphia

    Google Scholar 

  • Deaton M, Marlin J, Roberts A, Smith N, Harris A, Kelly J, Schroter C (2002) Antioxidant supplementation and pulmonary function at rest and exercise. Equine Veterinary Journal (Suppl. 34), 58–65

    Google Scholar 

  • Donawick J, Ramberg F, Paul R, Hiza A (1975) The diagnostic and prognostic value of lactate determinations in horses with acute abdominal crisis. J S Afr Vet Assoc 466:127

    Google Scholar 

  • Douglas C, Christie A, Patrick T, Natalie N, David J (2007) Exercise-induced alterations in pro-inflammatory cytokines and prostaglandin F2a in horses. Vet Immunol Immunopathol 118:263–269

    Article  Google Scholar 

  • Doumas T, Bayson D, Carter J, Peters T, Schaffer R (1981) Estimation of total serum protein. Clin Chem 27:1642–1643

    CAS  PubMed  Google Scholar 

  • European College of Equine Internal Medicine (ECEIM) (2010) Resident meeting, 6 February, Vienna

  • Faulker R, Meites S (1982) Sel Methods Clin Chem 9:185

    Google Scholar 

  • Flannery R, Little C, Hughes C, Curtis C, Caterson B, Jones S (2000) IL-6 and its soluble receptor augment aggrecanase-mediated proteoglycan catabolism in articular cartilage. J Biol 19:549–553

    CAS  Google Scholar 

  • Fridovich I (1995) Superoxide radical and superoxide dismutase. Annu Rev Biochem 64:97–112

    Article  CAS  PubMed  Google Scholar 

  • Friedman B, Young D (1997) Effects of disease on clinical laboratory tests, 3rd edn. AACC, Washington, D.C

    Google Scholar 

  • Goldring B (2000) Osteoarthritis and cartilage: the role of cytokines. Curr Rheumatol Rep 2:459–465

    Article  CAS  PubMed  Google Scholar 

  • Hamer R (1997) When exercise goes awry: exertional rhabdomyolysis. South Med J 90:548–551

    CAS  PubMed  Google Scholar 

  • Hargreaves J, Kronfeld S, Waldron N, Lopes A, Gay S, Saker E, Cooper L, Sklan J, Harris A (2002) Antioxidant status and muscle cell leakage during endurance exercise. Equine Vet J 34:116–121

    Article  Google Scholar 

  • Harris PA (1991) The equine rhabdomyolysis syndrome in the united kingdom: epidemiological and clinical descriptive information. Br Vet J 147:373–384

    CAS  PubMed  Google Scholar 

  • Harris A, Snow H (1991) Role of electrolyte imbalances in the pathophysiology of the equine rhabdomyolysis syndrome. In: Persson SGB, Lindholm A, Jeffcott LB (eds) Equine exercise physiology, 3rd edn. ICEEP, Davis, pp 435–442

    Google Scholar 

  • Henry B (1984) Clinical Diagnosis and management, 17th edition, Saunder Publisher. Elecsys triiodothyronine (11731360122) and thyroxine (12017709122), Cobas, 2005, Roche, Diagnostics, GmbH, D, 68298, Mannheim

  • Hosie D, Gould W, Hunter R, Low C, Munro R, Wilson C (1986) Acute myopathy in horses at grass in east and south east Scotland. Vet Rec 119:444–449

    Article  CAS  PubMed  Google Scholar 

  • Hutyra F, Marek J (1926) Diseases of the blood and blood-producing organs: paralytic hemoglobinemia. In: Mohler JR, Eichhorn A (eds) Special pathology and therapeutics of the diseases of domestic animals, first ed., Diseases of the urinary organs, blood and blood producing organs, spleen, metabolism, nervous system, organs of locomotion, and skin, vol III. Alexander Eger, Chicago, pp 146–162

    Google Scholar 

  • Jacobson H, Striker G, Klahr S (1991) Biochemical alterations in advanced uremic failure. In: Jacobson H, Striker G, Klahr S (eds) The principles and practice of nephrology. Decker, Philadelphia, pp 682–689

    Google Scholar 

  • Jenkins R (2000) Exercise and oxidative stress methodology: a critique. Am J Clin Nutr 72:670S–674

    CAS  PubMed  Google Scholar 

  • Jikko A, Wakisaka T, Iwamoto M et al (1998) Effects of interleukin-6 on proliferation and proteoglycan metabolism in articular chondrocyte cultures. Cell Biol Int 22:615–621

    Article  CAS  PubMed  Google Scholar 

  • Jones W (2003) Nutritional support for rhabdomyolysis. J Equine Vet Sci 23:325–326

    Google Scholar 

  • Jones S (2005) Directing transition from innate to acquired immunity: defining a role for IL-6. J Immunol 175:3463–3468

    CAS  PubMed  Google Scholar 

  • Kirschvink N, Art T, de Moffarts B, Smith N, Marlin D, Roberts C, Lekeux P (2002) Relationship between markers of blood oxidant status and physiological variables in trained and heaves-affected horses after exercise. Equine Vet J 34:159–164

    Article  Google Scholar 

  • Koracevic D, Koracevic G, Djordjevic V, Andrejevic S, Cosic V (2001) Method for the measurement of antioxidant activity in human fluids. J Clin Pathol 54:356–361

    Article  CAS  PubMed  Google Scholar 

  • Knochel P (1990) Catastrophic medical event with exhaustive exercise: white collar rhabdomyolysis. Kidney Int 38:709–719

    Article  CAS  PubMed  Google Scholar 

  • Lee J, Clarkson M (2003) Plasma creatine kinase activity and glutathione after eccentric exercise. Med Sci Sports Exerc 35:930–936

    Article  CAS  PubMed  Google Scholar 

  • Leeuwenburgh C, Heinecke W (2001) Oxidative stress and antioxidants in exercise. Curr Med Chem 8:829–838

    CAS  PubMed  Google Scholar 

  • Ley C, Ekman S, Ronéus B, Eloranta L (2009) Interleukin-6 and high mobility group box protein-1 in synovial membranes and osteochondral fragments in equine osteoarthritis. Res Vet Sci 86:490–497

    Article  CAS  PubMed  Google Scholar 

  • Lindholm A, Johansson HE, Kjaersgaard P (1974) Acute rhabdomyolysis (‘tying-up’) in Standardbred horses. Acta Vet Scand 15:325–337

    CAS  PubMed  Google Scholar 

  • Line L, Rust S (1995) Acute exertional rhabdomyolysis. Am Fam Physician 52:502–506

    CAS  PubMed  Google Scholar 

  • Marlin J, Fenn K, Smith N, Deaton D, Roberts A, Harris A, Dunster C, Kelly J (2002) Changes in circulatory antioxidant status in horses during prolonged exercise. J Nutr 132:1622S–1627S

    CAS  PubMed  Google Scholar 

  • Mates M (2000) Effects of antioxidant enzymes in the molecular control of reactive oxygen species toxicology. Toxicology 153:83–104

    Article  CAS  PubMed  Google Scholar 

  • Maughan J (1999) Role of micronutrients in sport and physical activity. Br Med Bull 55:683–690

    Article  CAS  PubMed  Google Scholar 

  • McKenzie C, Valberg J, Godden S, Pagan D, MacLeay M, Geor J, Carlson P (2003) Effect of dietary starch, fat and bicarbonate content on exercise responses and serum creatine kinase activity in equine recurrent exertional rhabdomyolysis. J Vet Intern Med 17:693–701

    Article  PubMed  Google Scholar 

  • Meginnis P (1957) Myositis (tying up) in race horses. J Am Vet Med Assoc 130:237–239

    CAS  PubMed  Google Scholar 

  • Mills C, Smith C, Harris C, Harris P (1997) Effect of allopurinol on the formation of reactive oxygen species during intense exercise in the horse. Res Vet Sci 62:11–16

    Article  CAS  PubMed  Google Scholar 

  • Montgomery C, Dymock J (1961) The determination of nitrite in water. Analyst 86:414–416

    CAS  Google Scholar 

  • Moorehead WR, Briggs HC (1974) Clinical chemistry, 20:1458 (As cited in Erba Manheim Kit for Ca estimation)

  • Nathalie K, Brieuc M, Pierre L (2008) The oxidant/antioxidant equilibrium in horses. Vet J 177:178–191

    Article  Google Scholar 

  • Nieman C (1997) Immune response to heavy exertion. J Appl Physiol 82:1385–1394

    CAS  PubMed  Google Scholar 

  • Nishikimi M, Appaji N, Yogi K (1972) The occurrence of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen. Biochem Biophys Res Commun 46:849–854

    Article  CAS  PubMed  Google Scholar 

  • Ohkawa H, Ohishi W, Yagi K (1979) Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 95:351–358

    Article  CAS  PubMed  Google Scholar 

  • Palencia P, Rivero L (2007) Atypical myopathy in two grazing horses in northern Spain. Vet Rec 161:346–348

    Article  CAS  PubMed  Google Scholar 

  • Paolisso G, D’Amore A, Giugliano D, Ceriello A, Varrichio M, Dı’Onofrio F (1993) Pharmacologic doses of vitamin E improve insulin action in healthy subjects and non-insulin-dependent diabetic patients. Am J Clin Nutr 57:650–6

    CAS  PubMed  Google Scholar 

  • Radostits M, Blood C, Gay C (2007) Veterinary medicine, a textbook of disease of cattle, sheep, pigs, goat and horses, 10th edn. SAUNDERS and ELSEVIER, Edinburgh

    Google Scholar 

  • Reitman M, Frankel S (1957) A colorimetric method for determination of serum glutamic oxaloacetic and glutamic pyruvic transaminase. Am J Clin Pathol 28:56

    CAS  PubMed  Google Scholar 

  • SAS (2002) Statistical analysis system. User’s guide. SAS, Cary

    Google Scholar 

  • Sen CK, Packer L (2000) Thiol homeostasis and supplements in physical exercise. Am J Clin Nutr 72(2 Suppl):653S–669S

    CAS  PubMed  Google Scholar 

  • Shingu M, Nagai Y, Isayama T, Naono T, Nobunaga M, Nagai Y (1993) The effects of cytokines on metalloproteinase inhibitors (TIMP) and collagenase production by human chondrocytes and TIMP production by synovial cells and endothelial cells. J Clin Exp Immunol 94:145–149

    Article  CAS  Google Scholar 

  • Shingu M, Miyauchi S, Nagai Y et al (1995) The role of IL-4 and IL-6 in IL-1-dependent cartilage matrix degradation. Br J Rheumatol 34:101–106

    Article  CAS  PubMed  Google Scholar 

  • Sies H (1991) Oxidative stress: introduction. In: Sies H (ed) Oxidative stress: oxidants and antioxidants. Academic, London, pp XV–XXII

    Google Scholar 

  • Sjaastad V, Hove K, Sand O (2004) Physiology of domestic animals. Scandinavian Veterinary Press, Oslo, pp 266–267

    Google Scholar 

  • Stewart J, Pettigrew A, Cochran A, Belknap J (2009) Indices of inflammation in the lung and liver in the early stages of the black walnut extract model of equine laminitis. Vet Immunol Immunopathol 129:254–260

    Article  CAS  PubMed  Google Scholar 

  • Tabacco A, Meiathini F, Moda E, Tarli P (1979) Simplified enzymic / colorimetric serum urea nitrogen determination. Clin Chem 25:336–337

    CAS  PubMed  Google Scholar 

  • Tietz N (1995) Clinical guide to laboratory tests, 3rd edn. W.B. Saunders, Philadelphia, PA

    Google Scholar 

  • Trinder P (1969) Determination of glucose in blood using glucose oxidase with alternative oxygen acceptor. Analytical Clinical. Biochemistry 6:24

    CAS  Google Scholar 

  • Valberg J, MacLeay M, Mickelsen R (1997) Exertional rhabdomyolysis and polysaccharide storage myopathy in horses. Compendium on Continuous Education for the Practical Veterinarian 19:1077–1085

    Google Scholar 

  • Valentine A, McDonough P, Chang F, Vonderchek A (2000) Polysaccharide storage myopathy in Morgan, Arabian, and Standardbred related horses and Welsh-cross ponies. Vet Pathol 37:193–196

    Article  CAS  PubMed  Google Scholar 

  • Valentine A, Van Saun J, Thompson N, Hintz F (2001) Role of dietary carbohydrate and fat in horses with equine polysaccharide storage myopathy. Am Vet Med Assoc 219:1537–1544

    Article  CAS  Google Scholar 

  • Van den Hoven R (1987) Some histochemical and biochemecial aspects of equine muscles with special respect to exertional myolysis. Thesis University of Utrecht, Netherlands

    Google Scholar 

  • Votion M, Linden A, Saegerman C, Engels P, Erpicum M, Thiry E, Delguste C, Rouxhet S, Demoulin V, Navet R, Sluse F, Serteyn D, Van Galen G, Amory H (2007) History and clinical features of atypical myopathy in horses in Belgium (2000–2005). J Vet Intern Med 21:1380–1391

    PubMed  Google Scholar 

  • Waller AP, Lindinger MI (2010) Nutritional aspects of post excercise skeletal muscle glycogen synthesis in horses: a comparative review. Equine Vet J 42:274–281

    Article  CAS  PubMed  Google Scholar 

  • Walsworth M, Kessler T (2001) Diagnosing exertional rhabdomyolysis: a brief review and report of two cases. Mil Med 166:275–277

    CAS  PubMed  Google Scholar 

  • Westermann M, DeSain-vanderVelden M, Van der Kolk H, Berger R, Wijnberg D, Koeman P, Wanders A, Lenstra A, Testerink N, Vaandrager B, Vianey-Saban C, Acquaviva-Bourdain C, Dorland L (2007) Equine biochemical multiple acyl CoA dehydrogenase deficiency (MADD) as a cause of rhabdomyolysis. Mol Genet Metab 91:362–369

    Article  CAS  PubMed  Google Scholar 

  • White A, Estrada M, Walker K, Wisnia P, Filgueira G, Valdes F, Araneda O, Behn C, Martinez R (2001) Role of exercise and ascorbate on plasma antioxidant capacity in thoroughbred race horses. Comp Biochem Physiol A Mol Integr Physiol 128:99–104

    Article  CAS  PubMed  Google Scholar 

  • Whitwell E, Harris P, Farrington G (1988) Atypical myoglobinuria: an acute myopathy in grazing horses. Equine Vet J 20:357–363

    Article  CAS  PubMed  Google Scholar 

  • Wootton P (1982) Microanalysis in medical biochemistry, 6th edn. Churchill, London, p 107

    Google Scholar 

  • Wroblewski F, Duean S (1955) Bestimmung der Aktivital der lactate dehydrogenase. Proc Soc Exp Biol 90:210–214

    CAS  PubMed  Google Scholar 

  • Young S, Thomas W, Friedman B, Pestaner C (1972) Effects of drugs on clinical laboratory tests. Clin Chem 18:1041–1303

    CAS  PubMed  Google Scholar 

  • Zentek J (1991) Myopathies in a riding horse stable. Tierärztl Prax 19:167–169

    CAS  PubMed  Google Scholar 

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Acknowledgements

This research was supported by the Deanship of Scientific Research, King Faisal University, Saudi Arabia.

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  1. Department of clinical studies, College of Veterinary Medicine and animal Resources, King Faisal University, P.O. Box: 1757, Al-Ahsa, 31982, Saudi Arabia

    Wael Mohamed EL-Deeb

  2. Department of Physiology, Biochemistry and Pharmacology, College of Veterinary Medicine and animal Resources, King Faisal University, P.O. Box: 1757, Al-Ahsa, 31982, Saudi Arabia

    Sabry M. El-Bahr

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Correspondence to Wael Mohamed EL-Deeb.

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EL-Deeb, W.M., El-Bahr, S.M. Investigation of selected biochemical indicators of Equine Rhabdomyolysis in Arabian horses: pro-inflammatory cytokines and oxidative stress markers. Vet Res Commun 34, 677–689 (2010). https://doi.org/10.1007/s11259-010-9439-5

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