Veterinary Research Communications

, Volume 34, Issue 5, pp 413–421 | Cite as

Characterization of a new protective antigen of Streptococcus canis

Original Article

Abstract

Streptococcus canis (S. canis), a lancefield group G streptococcus, is an opportunistic pathogen mainly found in dogs and cats. The study on pathogenesis and protective immune mechanism of S. canis is not clear. A new streptococcal protective antigen (SPA) was first identified from a genomic library of S. canis. SPA of S. canis (SPASc) contained a 1224-bp open reading frame which encoded a 407aa protein and a 34-aa signal sequence with a deduced molecular mass of 46.368 kDa. Protein analysis and BLAST result showed that SPASc was homologous to the SPA of Streptococcus. equi subsp. zooepidemicus, M protein Streptococcus. equi., and SPA of Streptococcus pyogenes. The protective response of SPASc antiserum was demonstrated by passive mouse protection. These studies suggested that SPASc might be an important component of vaccines to prevent S. canis infections.

Keywords

Streptococcal protective antigen S. canis Characterization Passive protection 

Supplementary material

11259_2010_9414_MOESM1_ESM.doc (69 kb)
Fig. 1DNA sequnence and deduced amino acid of SPASc. The sequence contains complete open reading frame with 1,224 nucleotides and deduced 407 amino acids. Underlined sequence means signal sequence. The sequence has been submitted to GeneBank and has the accession no. ACM47242 (DOC 69 kb)

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  1. 1.Department of Animal ScienceTianjin Agricultural UniversityTianjinChina

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