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Veterinary Research Communications

, Volume 33, Supplement 1, pp 141–144 | Cite as

Extended-spectrum β-lactamase production in E. coli strains isolated from clinical bovine mastitis

  • C. LocatelliEmail author
  • I. Caronte
  • L. Scaccabarozzi
  • R. Migliavacca
  • L. Pagani
  • P. Moroni
Abstract

Introduction

The existence and spread of cephalosporin-resistant Gram-negative bacteria in hospital intensive care units has been widely documented worldwide, including in Italy (Pagani et al. 2002). The most common and well-known resistance mechanism is the production of Extended Spectrum β-Lactamases (ESβL), which has been known since the 80’s. These enzymes are able to hydrolyze and inactivate the β-lactamic ring (Livermore 1995). In recent years attention on these enzymes has increased in veterinary medicine (Li et al. 2007). At the same time there has also been increased reporting of these enzymes both in companion animals in contact with humans and in livestock animals (Blanc et al. 2006; Liebana et al. 2006; Sidjabat et al. 2007) and in the environment in which they live (Blanc et al. 2006; Liebana et al. 2006). Therefore, epidemiological surveillance on the diffusion of ESβLs appears to be necessary both to ascertain the efficacy of available antimicrobial drugs and to assess...

Keywords

Conjugation E. coli ESβL Mastitis 

Abbreviations

ATCC

American Type Culture Collection

CFU

Colony Forming Unit

ESβL

Extended spectrum β-lactamases

I.p.

Isoelectric point

IEF

Isoelectric focusing

MH

Müeller-Hinton

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • C. Locatelli
    • 1
    Email author
  • I. Caronte
    • 1
  • L. Scaccabarozzi
    • 1
  • R. Migliavacca
    • 2
  • L. Pagani
    • 2
  • P. Moroni
    • 1
  1. 1.Dipartimento di Patologia Animale, Igiene e Sanità Pubblica VeterinariaUniversità degli Studi di MilanoMilanoItaly
  2. 2.Dipartimento di Scienze Morfologiche, Eidologiche e ClinicheUniversità degli Studi di PaviaPaviaItaly

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