Characterization of Dermanyssus gallinae (Acarina: Dermanissydae) by sequence analysis of the ribosomal internal transcribed spacer regions

  • L. Potenza
  • M. A. Cafiero
  • A. Camarda
  • G. La Salandra
  • L. Cucchiarini
  • M. Dachà
Original article

Abstract

In the present work mites previously identified as Dermanyssus gallinae De Geer (Acari, Mesostigmata) using morphological keys were investigated by molecular tools. The complete internal transcribed spacer 1 (ITS1), 5.8S ribosomal DNA, and ITS2 region of the ribosomal DNA from mites were amplified and sequenced to examine the level of sequence variations and to explore the feasibility of using this region in the identification of this mite. Conserved primers located at the 3’end of 18S and at the 5’start of 28S rRNA genes were used first, and amplified fragments were sequenced. Sequence analyses showed no variation in 5.8S and ITS2 region while slight intraspecific variations involving substitutions as well as deletions concentrated in the ITS1 region. Based on the sequence analyses a nested PCR of the ITS2 region followed by RFLP analyses has been set up in the attempt to provide a rapid molecular diagnostic tool of D. gallinae.

Keywords

Dermanyssus gallinae Internal transcribed spacer Mites Polymerase chain reaction 

Notes

Acknowledgements

This work was supported by a research grant (Ricerca Corrente 2006) from Ministry of Health, Italy

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • L. Potenza
    • 1
  • M. A. Cafiero
    • 2
  • A. Camarda
    • 3
  • G. La Salandra
    • 2
  • L. Cucchiarini
    • 1
    • 4
  • M. Dachà
    • 4
  1. 1.Università degli Studi di Urbino “Carlo Bo” Dipartimento di Scienze Biomolecolari2 UrbinoItaly
  2. 2.Istituto Zooprofilattico Sperimentale della Puglia e della BasilicataFoggiaItaly
  3. 3.Dipartimento di Sanità Pubblica e ZootecniaUniversità degli Studi di BariBariItaly
  4. 4.Centro Integrato di RicercheUniversità Campus Bio-MedicoRomaItaly

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