Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Genotyping of Chlamydophila psittaci strains derived from avian and mammalian species

  • 137 Accesses

  • 2 Citations


Eleven Chlamydophila (formerly Chlamydia) psittaci strains derived from avian and mammalian species (two from dairy cows, one from duck, three from sheep, three from wild birds, and two from pigs) were identified as genotype C by outer membrane protein A gene sequencing. Genotype C had been preferentially associated with waterfowl. This paper suggests that mammals may represent an underestimated source for genotype C strains and for human psittacosis cases.

This is a preview of subscription content, log in to check access.

Fig. 1
Fig. 2


  1. Denamur, E., Sayada, C., Souriau, A., Orfila, J., Rodolakis, A., Elion, J., 1991. Restriction pattern of the major outer-membrane protein gene provides evidence for a homogeneous invasive group among ruminant isolates of Chlamydia psittaci. Journal of General Microbiology, 137, 2525–2530

  2. Geens, T., Desplanques, A., Van Loock, M., Bonner, B.M., Kaleta, E.F., Magnino, S., Andersen, A.A., Everett, K.D., Vanrompay, D., 2005. Sequencing of the Chlamydophila psittaci ompA gene reveals a new genotype, E/B, and the need for a rapid discriminatory genotyping method. Journal of Clinical Microbiology, 43, 2456–2461. doi:10.1128/JCM.43.5.2456-2461.2005

  3. Heddema, E.R., van Hannen, E.J., Duim, B., Vandenbroucke-Grauls, C.M., Pannekoek, Y., 2006. Genotyping of Chlamydophila psittaci in human samples. Emerging Infectious Diseases, 12, 1989–1990

  4. Kumar, S., Tamura, K., Nei, M., 2004. MEGA3: Integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment. Briefings in Bioinformatics, 5, 150–163. doi:10.1093/bib/5.2.150

  5. Thompson, J.D., Gibson, T.J., Plewniak, F., Jeanmougin, F., Higgins, D.G., 1997. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research, 25, 4876–4882. doi:10.1093/nar/25.24.4876

  6. Vanrompay, D., Butaye, P., Sayada, C., Ducatelle, R., Haesebrouck, F., 1997. Characterization of avian Chlamydia psittaci strains using omp1 restriction mapping and serovar-specific monoclonal antibodies. Research in Microbiology, 148, 327–333. doi: 10.1016/S0923-2508(97)81588-4

  7. Zhang, Y.X., Morrison, S.G., Caldwell, H.D., Baehr, W., 1989. Cloning and sequence analysis of the major outer membrane protein genes of two Chlamydia psittaci strains. Infection and Immunity, 57, 1621–1625

Download references


This work was supported by the National Natural Science Foundation of China (No. 30500369).

Author information

Correspondence to Q. Duan.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Song, L., Li, Y., Liu, G. et al. Genotyping of Chlamydophila psittaci strains derived from avian and mammalian species. Vet Res Commun 33, 577–580 (2009).

Download citation


  • Chlamydia
  • Chlamydophila psittaci
  • Genotyping
  • ompA