Acute phase proteins in cattle after exposure to complex stress
- 804 Downloads
Stressors such as weaning, mixing and transportation have been shown to lead to increased blood concentrations of acute phase proteins (APP), including serum amyloid A (SAA) and haptoglobin, in calves. This study was therefore undertaken to assess whether SAA and haptoglobin levels in blood mirror stress in adult cattle. Six clinically healthy Holstein cows and two Holstein heifers were transported for four to six hours to a research facility, where each animal was housed in solitary tie stalls. Blood samples for evaluation of leukocyte counts and serum SAA and haptoglobin concentrations were obtained before (0-sample) and at 8, 24 and 48 hours after the start of transportation. Upon arrival the animals gave the impression of being anxious, and they appeared to have difficulty coping with isolation and with being tied on the slippery floors of the research stable. Serum concentrations of SAA and haptoglobin increased significantly in response to the stressors (P < 0.01 and 0.05 at 48 hours, respectively). Additionally, the animals had transient neutrophilia at 8 and 24 hours (P < 0.05). In conclusion, the results of the study suggest that SAA and haptoglobin may serve as markers of stress in adult cattle.
KeywordsAcute phase proteins Cattle Haptoglobin Housing Transport Serum amyloid A Stress
The authors wish to thank the Division of Laboratory Animal Science and Welfare at Faculty of Life Sciences, University of Copenhagen for housing the animals and helping with the experiments, and the Central Laboratory at Faculty of Life Sciences, University of Copenhagen for performing laboratory analyses. The Danish Cattle Federation provided funding for the study.
- Fischer A.D., Crowe M.A., Ó’Nualláin E.M., Monaghan M.L., Larkin J.A., O’Kiely P. and Enright W.J., 1997. Effects of cortisol on in vitro interferon-g production, acute-phase proteins, growth, and feed intake in a calf castration model. Journal of Animal Science, 75, 1041–1047Google Scholar
- Heegaard P.M.H., Godson D.L., Toussaint M.J.M., Tjørnehøj K., Larsen L.E., Viuff B. and Rønsholt L., 2000. The acute phase reponse of haptoglobin and serum amyloid A (SAA) in cattle undergoing experimental infection with bovine respiratory syncytial virus. Veterinary Immunology and Immunopathology, 77, 151–159PubMedCrossRefGoogle Scholar
- Morrow-Tesch J. and Whitehead K., 1998. The effect of blood sampling site on measures of stress in dairy cattle. Proceedings New Zealand Society of Animal Production, 58, 218–219Google Scholar
- Weber P.S., Toelboell T., Chang L.C., Tirrell J.D., Saama P.M., Smith G.W. and Burton J.L., 2004. Mechanisms of glucocorticoid-induced down-regulation of neutrophil L-selectin in cattle: evidence for effects at the gene-expression level and primarily on blood neutrophils. Journal of Leukocyte Biology, 75, 815–827PubMedCrossRefGoogle Scholar