Identification of intimin alleles in pathogenic Escherichia coli by PCR-restriction fragment length polymorphism analysis
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A rapid two-step identification method based on PCR-RFLP analysis of the intimin gene was developed to differentiate specific alleles in pathogenic Escherichia coli. This technique, tested on isolates eae-positive, accurately detects eae and resolves alleles encoding the α1, α2, β, γ1, γ2/θ, κ, ɛ, ζ, and ι intimin variants.
KeywordsEscherichia coli Intimin PCR-RFLP
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