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Veterinary Research Communications

, Volume 32, Issue 1, pp 1–5 | Cite as

Identification of intimin alleles in pathogenic Escherichia coli by PCR-restriction fragment length polymorphism analysis

  • C. Tramuta
  • P. RobinoEmail author
  • E. Oswald
  • P. Nebbia
Short Communication

Abstract

A rapid two-step identification method based on PCR-RFLP analysis of the intimin gene was developed to differentiate specific alleles in pathogenic Escherichia coli. This technique, tested on isolates eae-positive, accurately detects eae and resolves alleles encoding the α1, α2, β, γ1, γ2/θ, κ, ɛ, ζ, and ι intimin variants.

Keywords

Escherichia coli Intimin PCR-RFLP 

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Copyright information

© Springer Science + Business Media B.V. 2007

Authors and Affiliations

  1. 1.Dipartimento di Produzioni Animali, Epidemiologia ed EcologiaUniversità degli studi di Torino, Facoltà di Medicina VeterinariaGrugliascoItaly
  2. 2.UMR960 INRA de Microbiologie Moleculaire, Ecole Nationale VétérinaireToulouseFrance

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